Skip to main content
SpringerLink
Log in

Molecular, cytogenetic and morphological characterization of somatic hybrids of dihaploid Solanum tuberosum and diploid S. brevidens

  • Published:
Theoretical and Applied Genetics Aims and scope Submit manuscript

Summary

Fifty-eight somatic hybrid plants, produced both by chemical (11) and electrical fusion (47) of protoplasts of dihaploid Solanum tuberosum and S. brevidens, have been analysed by molecular, cytological and morphological methods. The potentially useful euploid plants constituted 34% of the total, of which 20% were tetraploid and 14% hexaploid; the remainder were aneuploid at the tetraploid, hexaploid and octoploid levels. Analysis of chloroplast DNA showed that 55% of hybrids contained chloroplasts from S. brevidens and 45% from S. tuberosum. Hexaploids, the products of three protoplasts fusing together, were analyzed with specific DNA probes, and this revealed that nuclear genome dosages could be either 2∶1 S. tuberosum∶S. brevidens, or vice-versa. Chloroplast types of hexaploids were not influenced by nuclear genome dosage, and all six possible combinations of genome dosage and chloroplast types were found amongst tetraploids and hexaploids. To examine the morphology of the hybrid population and its possible relation to the chromosome number and chloroplast DNA type, 18 morphological characteristics were measured on greenhouse-grown plants and analyzed by principal component and canonical variate analyses. Both analyses showed that nuclear ploidy has the most prominent influence on the overall morphology of the hybrids. Differential parental genome expression in the morphology of the hybrids is discussed. These results provide useful data on the range of genetic combinations that can be expected to occur amongst somatic hybrid plants.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Austin S, Baer MA, Helgeson JP (1985) Transfer of resistance to potato leaf roll virus from Solanum brevidens into Solanum tuberosum by somatic fusion. Plant Sci 39:75–82

    Google Scholar 

  • Austin S, Ehlenfeldt MK, Baer MA, Helgeson JP (1986) Somatic hybrids produced by protoplast fusion between S. tuberosum and S. brevidens: phenotypic variation under field conditions. Theor Appl Genet 71:628–690

    Google Scholar 

  • Barsby TL, Shepard JF, Kemble RJ, Wong R (1984) Somatic hybridization in the genus Solanum: S. tuberosum and S. brevidens. Plant Cell Rep 3:165–167

    Google Scholar 

  • Belliard G, Vedel F, Pelletier G (1979) Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Nature 281:401–403

    Google Scholar 

  • Borisjuk NV, Momot VP, Gleba Y (1988) Novel class of rDNA repeat units in somatic hybrids between Nicotiana and Atropa. Theor Apl Genet 76:108–112

    Google Scholar 

  • Chupeau Y, Missonier C, Hommel M-C, Goujand J (1978) Somatic hybrids of plants by fusion of protoplasts. Observations on the model system “Nicotiana glauca — Nicotiana langsdorfii”. Mol Gen Genet 165:239–245

    Google Scholar 

  • Church GM, Gilbert W (1984) Genomic sequencing. Proc Natl Acad Sci USA 81:1991–1995

    CAS  PubMed  Google Scholar 

  • Dellaporta SL, Wood J, Hicks JB (1983) A plant DNA minipreparation: version II. Plant Mol Biol Rep 1:19–21

    CAS  Google Scholar 

  • Feinberg AP, Vogelstein H (1983) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132:6–13

    CAS  PubMed  Google Scholar 

  • Fish N, Karp A, Jones MGK (1987) Improved isolation of dihaploid S. tuberosum protoplasts and the production of somatic hybrids between dihaploid S. tuberosum and S. brevidens. In Vitro 23:575–580

    Google Scholar 

  • Fish N, Karp A, Jones MGK (1988 a) Production of somatic hybrids by electrofusion in Solanum. Theor Appl Genet 76:260–266

    Google Scholar 

  • Fish N, Steele SH, Jones MGK (1988 b) Field assessment of dihaploid Solanum tuberosum and Solanum brevidens somatic hybrids. Theor Appl Genet 76:880–886

    Google Scholar 

  • Genstat 5 Committee (1987) Genstat 5, Reference manual. Clarendon Press, Oxford

    Google Scholar 

  • Gibson RN, Jones MGK, Fish N (1988) Resistance to potato leaf roll virus and potato virus Y in somatic hybrids between dihaploid S. tuberosum and S. brevidens. Theor Appl Genet 76:113–117

    Google Scholar 

  • Gleba YY, Sytnik KM (1984) Protoplast fusion. Genetic engineering in higher plants. (Monographs on Theoretical Applied Genetics) Springer, Berlin Heidelberg New York

    Google Scholar 

  • Gleddie S, Keller WA, Setterfield G (1986) Production and characterization of somatic hybrids between Solanum melongena L. and S. sisymbriifolium Lam. Theor Appl Genet 71:613–621

    Google Scholar 

  • Gressel J, Cohen N, Binding H (1984) Somatic hybridization of an atrazine-resistant biotype of Solanum nigrum with Solanum tuberosum. 2. Segregation of plastomes. Theor Appl Genet 67:131–134

    Google Scholar 

  • Handley LW, Nickels RL, Cameron MW, Moore PP, Sink KC (1986) Somatic hybrid plants between Lycopersicon esculentum and Solanum lycopersicoides. Theor Appl Genet 71:691–697

    Google Scholar 

  • Hosaka K (1986) Who is the mother of the potato? — restriction endonuclease analysis of chloroplast DNA of cultivated potatoes. Theor Appl Genet 72:606–618

    Google Scholar 

  • Jones MGK (1988) Fusing plant protoplasts. Trends Biotechnol 6:153–158

    Google Scholar 

  • Jones RAC (1979) Resistance of potato leaf roll virus in Solanum brevidens. Potato Res 22:149–152

    Google Scholar 

  • Karp A, Nelson RS, Thomas E, Bright SWJ (1982) Chromosome variation in protoplast-derived potato plants. Theor Appl Genet 63:265–272

    Google Scholar 

  • Maniatis T, Fritsch EF, Sambrock J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor/NY

    Google Scholar 

  • Mignery G, Pikaard C, Hannapel D, Park W (1984) Isolation and sequence analysis of cDNA clones for the major potato tuber protein, patatin. Nucleic Acids Res 12:7987–8000

    Google Scholar 

  • Morgan A, Maliga P (1987) Rapid chloroplast segregation and recombination of mitochondrial DNA in Brassica cybrids. Mol Gen Genet 209:240–246

    Google Scholar 

  • Nagy F, Torok I, Maliga P (1981) Extensive rearrangements of the mitochondrial DNA in somatic hybrids of Nicotiana tabacum and Nicotiana knightiana. Mol Gen Genet 183:437–439

    Google Scholar 

  • NIAB (1975) Guide to the identification of potato varieties. National Institute of Agricultural Botany, Cambridge

    Google Scholar 

  • Ozias-Akins P, Pring DR, Vasil IK (1987) Rearrangements in the mitochondrial genome of somatic hybrid cell lines of Pennisetum americanum (L). K Schum + Panicum maximum Jacq. Theor Appl Genet 74:15–20

    Google Scholar 

  • Pehu E, Veilleux RE, Hilu KH (1987) Cluster analysis of antherderived plants of Solanum phureja (Solanaceae) based on morphological characteristics. Am J Bot 74:47–52

    Google Scholar 

  • Pental D, Hamill JD, Pirrie A, Cocking EC (1986) Somatic hybridization of Nicotiana tabacum and Petunia hybrida. Mol Gen Genet 202:342–347

    Google Scholar 

  • Rosen B, Hallden C, Heneen WK (1988) Diploid Brassica napus somatic hybrids: Characterization of nuclear and organellar DNA. Theor Appl Genet 76:197–203

    Google Scholar 

  • Santos RF, Handro W (1983) Morphological patterns in Petunia hybrida plants regenerated from tissue cultures and differing by their ploidy. Theor Appl Genet 66:55–60

    Google Scholar 

  • Saul MW, Potrykus I (1984) Species-specific repetitive DNA used to identify interspecific somatic hybrids. Plant Cell Rep 3:65–67

    Google Scholar 

  • Schuman U, Koblitz H (1985) Anwendung der Protoplastentechnologie in Züchtungsforschung der Kartoffel. Eine Literaturübersicht. Kulturpflanze 33:73–90

    Google Scholar 

  • Sree Ramulu K, Devreux M, Ancora G, Laneri U (1976) Chimerism in Lycopersicum peruvianum plants regenerated from in vitro cultures of anthers and stem internodes. Z Pflanzenzucht 76:299–319

    Google Scholar 

  • Sundberg E, Glimelius K (1986) A method for production of interspecific hybrids within Brassicaceae via somatic hybridization using resynthesis of Brassica napus resynthesised by protoplast fusion. Plant Sci 43:155–162

    Google Scholar 

  • Sundberg E, Landgren M, Glimelius K (1987) Fertility and chromosome stability in Brassica napus resynthesised by protoplast fusion. Theor Appl Genet 75:96–104

    Google Scholar 

  • Tempelaar MJ, Jones MGK (1985 a) Fusion characteristics of plant protoplasts in electric fields. Planta 165:205–216

    Google Scholar 

  • Tempelaar MJ, Jones MGK (1985 b) Directed electrofusion between protoplasts with different responses in a mass fusion system. Plant Cell Rep 4:92–95

    Google Scholar 

  • Tempelaar MJ, Duyst A, Vlas SY de, Krol G, Symonds C, Jones MGK (1987) Modulation and direction of the electrofusion response in plant protoplasts. Plant Sci 48:99–105

    Google Scholar 

  • Twell D, Ooms G (1988) Structural diversity of the patatin gene family in potato cv Desiree. Mol Gen Genet 212:325–336

    Google Scholar 

  • Uchimiya H, Ohgauava T, Kalo H, Akiyama T, Harada H (1983) Detection of two different nuclear genomes in parasexual hybrids by ribosomal RNA gene analysis. Theor Appl Genet 64:117–118

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Biochemistry Department, Rothamsted Experimental Station, AFRC Institute of Arable Crops Research, AL5 2JQ, Harpenden, Herts., UK

    E. Pehu, A. Karp, K. Moore, S. Steele, R. Dunckley & M. G. K. Jones

Authors
  1. E. Pehu
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. A. Karp
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. K. Moore
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. S. Steele
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. R. Dunckley
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. M. G. K. Jones
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Communicated by Yu. Gleba

Rights and permissions

Reprints and permissions

About this article

Cite this article

Pehu, E., Karp, A., Moore, K. et al. Molecular, cytogenetic and morphological characterization of somatic hybrids of dihaploid Solanum tuberosum and diploid S. brevidens . Theoret. Appl. Genetics 78, 696–704 (1989). https://doi.org/10.1007/BF00262566

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00262566

Key words

Search

Navigation