Abstract
This study was conducted to synthesize γ-AlOOH (bohemite)-based nanocomposites (NCs) of Au/γ-AlOOH-NC and its functionalized derivative using chitosan (Au/γ-AlOOH/Ctn-NC) and with the help of one-step Mentha piperita. The physicochemical characteristics of the NCs were investigated. In addition, biomedical properties, such as antibacterial activity under in vitro and in vivo conditions, and cell viability were assessed. Wound healing activity on infected wounds and histological parameters were assessed. The gene expressions of TNF-α, Capase 3, Bcl-2, Cyclin-D1 and FGF-2 were investigated. The TEM and FESEM images showed the sheet-like structure for bohemite in Au/γ-AlOOH-NC with Au nanoparticles in a range of 14–15 nm. The elemental analysis revealed the presence of carbon, oxygen, aluminum, and Au elements in the as-synthesized Au/γ-AlOOH. The results for toxicity showed that the produced nanocomposites did not show any cytotoxicity. Biomedical studies confirmed that Au/γ-AlOOH-NC and Au/γ-AlOOH/Ctn-NC have anti-bacterial properties and could expedite the wound healing process in infected wounds by an increase in collagen biosynthesis. The administration of ointment containing Au/γ-AlOOH-NC and Au/γ-AlOOH/Ctn-NC decreased the expressions of TNF-α, and increased the expressions of Capase 3, Bcl-2, Cyclin-D1 and FGF-2. The novelty of this study was that bohemite and Au nanoparticles can be used as a dressing to accelerate the wound healing process. In green synthesis of Au/γ-AlOOH-NC, phytochemical compounds of the plant extract are appropriate reagents for stabilization and the production of Au/γ-AlOOH-NC. Therefore, the new bohemite-based NCs can be considered as candidate for treatment of infected wounds after future clinical studies.
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The data used to support the findings of this study are available from the corresponding author upon request.
References
Abd El-Hack ME, El-Saadony MT, Shafi ME, Zabermawi NM, Arif M, Batiha GE, Khafaga AF, Abd El-Hakim YM (2020) Antimicrobial and antioxidant properties of chitosan and its derivatives and their applications: a review. Int J Biol Macromol 1:2726–2744. https://doi.org/10.1016/j.ijbiomac.2020.08.153
Aleksandr L, Alexander P, Olga B, Sergey K, Irena G (2018) Synthesis of antimicrobial AlOOH–Ag composite nanostructures by water oxidation of bimetallic Al–Ag nanoparticles. RSC Adv 8:36239–36244. https://doi.org/10.1039/C8RA04173C
Al-Musawi S, Albukhaty S, Al-Karagoly H, Sulaiman GM, Alwahibi MS, Dewir YH, Soliman DA, Rizwana H (2020) Antibacterial activity of honey/chitosan nanofibers loaded with capsaicin and gold nanoparticles for wound dressing. Molecules 17:4770. https://doi.org/10.3390/molecules25204770
Andretto V, Rosso A, Briançon S, Lollo G (2021) Nanocomposite systems for precise oral delivery of drugs and biologics. Drug Deliv Transl Res 11(2):445–470. https://doi.org/10.1007/s13346-021-00905-w
Bak DH, Lee E, Lee BC, Choi MJ, Kwon TR, Kim JH, Park BC, Lee K, Kim S, Na J, Kim BJ (2020) Boehmite enhances hair follicle growth via stimulation of dermal papilla cells by upregulating β-catenin signalling. Exp Dermatol 29:341–348. https://doi.org/10.1111/exd.14051
Behzad F, Jafarirad S, Samadi A, Barzegar A (2020) A systematic investigation on spectroscopic, conformational, and interactional properties of polypeptide/nanomaterial complex: effects of bio-based synthesized maghemite nanocomposites on human serum albumin. Soft Mater 18:471–486. https://doi.org/10.1080/1539445X.2020.1716800
Buldain D, Gortari Castillo L, Marchetti ML, Julca Lozano K, Bandoni A, Mestorino N (2021) Modeling the growth and death of Staphylococcus aureus against Melaleuca armillaris essential oil at different pH conditions. Antibiotics 10:222. https://doi.org/10.3390/antibiotics10020222
Daghian SG, Farahpour MR, Jafarirad S (2021) Biological fabrication and electrostatic attractions of new layered silver/talc nanocomposite using Lawsonia inermis L. and its chitosan-capped inorganic/organic hybrid: investigation on acceleration of Staphylococcus aureus and Pseudomonas aeruginosa infected wound healing. Mater Sci Eng 128:112294. https://doi.org/10.1016/j.msec.2021.112294
Das P, Horton R (2016) Antibiotics: achieving the balance between access and excess. Lancet 387:102–104. https://doi.org/10.1016/S0140-6736(15)00729-1
de Araújo R, Lôbo M, Trindade K, Silva DF, Pereira N (2019) Fibroblast growth factors: a controlling mechanism of skin aging. Skin Pharmacol Physiol 4:275–282. https://doi.org/10.1159/000501145
Desjardins-Park HE, Foster DS, Longaker MT (2018) Fibroblasts and wound healing: an update. Regen Med 13:491–495. https://doi.org/10.2217/rme-2018-0073
Farahpour MR, Hesaraki S, Faraji D, Zeinalpour R, Aghaei M (2017) Hydroethanolic Allium sativum extract accelerates excision wound healing: evidence for roles of mast-cell infiltration and intracytoplasmic carbohydrate ratio. Braz J Pharm Sci 20:53–62. https://doi.org/10.1590/s2175-97902017000115079
Forest V, Pailleux M, Pourchez J, Boudard D, Tomatis M, Fubini B, Sennour M, Hochepied JF, Grosseau P, Cottier M (2014) Toxicity of boehmite nanoparticles: impact of the ultrafine fraction and of the agglomerates size on cytotoxicity and pro-inflammatory response. Inhal Toxico 26(9):545–553. https://doi.org/10.3109/08958378.2014.925993
Gharehpapagh AC, Farahpour MR, Jafarirad S (2021) The biological synthesis of gold/perlite nanocomposite using Urtica dioica extract and its chitosan-capped derivative for healing wounds infected with methicillin-resistant Staphylococcus aureus. Int J Biol Macromol 183:447–456. https://doi.org/10.1016/j.ijbiomac.2021.04.150
Hernández Martínez SP, Rivera González TI, Franco Molina MA, Bollain y Goytia JJ, Martínez Sanmiguel JJ, Zárate Triviño DG, Rodríguez Padilla C (2019) A novel gold calreticulin nanocomposite based on chitosan for wound healing in a diabetic mice model. Nanomaterials 8:75. https://doi.org/10.3390/nano9010075
Islam MS, Islam JM, Rahman MF, Rahman MM, Khan MA (2021) Gelatin-based instant gel-forming volatile spray for wound-dressing application. Prog Biomater 10(3):235–243. https://doi.org/10.1007/s40204-021-00166-3
Jafarirad S, Taghizadeh PM, Divband B (2020) Biosynthesis, characterization and structural properties of a novel kind of Ag/ZnO nanocomposites in order to increase its biocompatibility across human A549 cell line. Bio Nano Sci 10:42–53. https://doi.org/10.1007/s12668-019-00685-1
Jamshed A, Jabeen Q (2022) Pharmacological evaluation of Mentha piperita against urolithiasis: an in vitro and in vivo study. Dose Response 20(1):1–15
Joshi AS, Singh P, Mijakovic I (2020) Interactions of gold and silver nanoparticles with bacterial biofilms: molecular interactions behind inhibition and resistance. Int J Mol Sci 21:7658. https://doi.org/10.3390/ijms21207658
Kaiser P, Wächter J, Windbergs M (2021) Therapy of infected wounds: overcoming clinical challenges by advanced drug delivery systems. Drug Deliv Transl Res 11:1545–1567. https://doi.org/10.1007/s13346-021-00932-7
Lee WS, Cho HJ, Kim E, Huh YH, Kim HJ, Kim B, Kang T, Lee JS, Jeong J (2019) Bioaccumulation of polystyrene nanoplastics and their effect on the toxicity of Au ions in zebrafish embryos. Nanoscale 11(7):3173–3185
Li Q, Lu F, Zhou G, Yu K, Lu B, Xiao Y, Dai F, Wu D, Lan G (2017) Silver inlaid with gold nanoparticle/chitosan wound dressing enhances antibacterial activity and porosity, and promotes wound healing. Biomacromol 18:3766–3775. https://doi.org/10.1021/acs.biomac.7b01180
Makabenta JM, Nabawy A, Li CH, Schmidt-Malan S, Patel R, Rotello VM (2021) Nanomaterial-based therapeutics for antibiotic-resistant bacterial infections. Nat Rev Microbiol 19:23–36. https://doi.org/10.1038/s41579-020-0420-1
Mir M, Ali MN, Barakullah A, Gulzar A, Arshad M, Fatima S, Asad M (2018) Synthetic polymeric biomaterials for wound healing: a review. Prog Biomater 7(1):1–21. https://doi.org/10.1007/s40204-018-0083-4
Modarresi M, Farahpour MR, Baradaran B (2019) Topical application of Mentha piperita essential oil accelerates wound healing in infected mice model. Inflammopharmacology 27:531–537. https://doi.org/10.1007/s10787-018-0510-0
Moetamedipoor SA, Saharkhiz MJ, Khosravi AR, Jowkar A (2021) Essential oil chemical diversity of Iranian mints. Ind Crops Prod 172:114039. https://doi.org/10.1016/j.indcrop.2021.114039
Mohan K, Ganesan AR, Muralisankar T, Jayakumar R, Sathishkumar P, Uthayakumar V, Chandirasekar R, Revathi N (2020) Recent insights into the extraction, characterization, and bioactivities of chitin and chitosan from insects. Trends Food Sci Technol 105:17–42. https://doi.org/10.1016/j.tifs.2020.08.016
Mossahebi-Mohammadi M, Quan M, Zhang JS, Li X (2020) FGF signaling pathway: a key regulator of stem cell pluripotency. Front Cell Dev Biol 8:79. https://doi.org/10.3389/fcell.2020.00079
Nagai N, Ihara K, Itoi A, Kodaira T, Takashima H, Hakuta Y, Bando KK, Itoh N, Mizukami F (2012) Fabrication of boehmite and Al2O3 nonwovens from boehmite nanofibres and their potential as the sorbent. J Mater Chem 22:21225–21231. https://doi.org/10.1039/C2JM33914E
Naraginti S, Kumari PL, Das RK, Sivakumar A, Patil SH, Andhalkar VV (2016) Amelioration of excision wounds by topical application of green synthesized, formulated silver and gold nanoparticles in albino Wistar rats. Mater Sci Eng 62:293–300. https://doi.org/10.1016/j.msec.2016.01.069
Negut I, Grumezescu V, Grumezescu AM (2018) Treatment strategies for infected wounds. Molecules 23(9):2392. https://doi.org/10.3390/molecules23092392
Paglia G, Božin ES, Billinge SJ (2006) Fine-scale nanostructure in γ-Al2O3. Chem 18:3242–3248. https://doi.org/10.1021/cm060277j
Pajerski W, Ochonska D, Brzychczy-Wloch M, Indyka P, Jarosz M, Golda-Cepa M, Sojka Z, Kotarba A (2019) Attachment efficiency of gold nanoparticles by Gram-positive and Gram-negative bacterial strains governed by surface charges. J Nanopart Res 21:1–2. https://doi.org/10.1007/s11051-019-4617-z
Parvekar P, Palaskar J, Metgud S, Maria R, Dutta S (2020) The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of silver nanoparticles against Staphylococcus aureus. Biomater Investig Dent 7:105–109. https://doi.org/10.1080/26415275.2020.1796674
Popgeorgiev N, Jabbour L, Gillet G (2018) Subcellular localization and dynamics of the Bcl-2 family of proteins. Front Cell Dev Biol 13:13. https://doi.org/10.3389/fcell.2018.00013
Pourkarim R, Farahpour MR, Rezaei SA (2022) Comparison effects of platelet-rich plasma on healing of infected and non-infected excision wounds by the modulation of the expression of inflammatory mediators: experimental research. Eur J Trauma Emerg Surg 12:1–9. https://doi.org/10.1007/s00068-022-01907-0
Rashed HH (2017) Antibacterial activity of boehmite nanoparticles synthesized by arc discharge in deionized water technique. Al-Nahrain J Sci 20:58–65. https://doi.org/10.22401/JNUS.20.1.08
Raziyeva K, Kim Y, Zharkinbekov Z, Kassymbek K, Jimi S, Saparov A (2021) Immunology of acute and chronic wound healing. Biomolecules 11:700. https://doi.org/10.3390/biom11050700
Rezaei Z, Jafarirad S, Kosari-Nasab M (2019) Modulation of secondary metabolite profiles by biologically synthesized MgO/perlite nanocomposites in Melissa officinalis plant organ cultures. J Hazard Mater 380:120878. https://doi.org/10.1016/j.jhazmat.2019.120878
Ritsu M, Kawakami K, Kanno E, Tanno H, Ishii K, Imai Y, Maruyama R, Tachi M (2017) Critical role of tumor necrosis factor-α in the early process of wound healing in skin. J Dermatol Dermatol Surg 21:14–19. https://doi.org/10.1016/j.jdds.2016.09.001
Sathiyaraj S, Suriyakala G, Gandhi AD, Babujanarthanam R, Almaary KS, Chen TW, Kaviyarasu K (2021) Biosynthesis, characterization, and antibacterial activity of gold nanoparticles. J Infect Public Health 14:1842–1847. https://doi.org/10.1016/j.jiph.2021.10.007
Shen C, Li J, Zhang Y, Li Y, Shen G, Zhu J, Tao J (2017) Polyethylenimine-based micro/nanoparticles as vaccine adjuvants. Int J Nanomed 12:5443–5460
Shkir M, Yahia IS, Ganesh V, Bitla Y, Ashraf IM, Kaushik A, Alfaify S (2018) A facile synthesis of Au-nanoparticles decorated PbI2 single crystalline nanosheets for optoelectronic device applications. Sci Rep 8:1–10. https://doi.org/10.1038/s41598-018-32038-5
Silva H (2020) A descriptive overview of the medical uses given to Mentha aromatic herbs throughout history. Biology 9:484. https://doi.org/10.3390/biology9120484
Slavin YN, Asnis J, Häfeli UO, Bach H (2017) Metal nanoparticles: understanding the mechanisms behind antibacterial activity. J Nanobiotechnol 15:1–20. https://doi.org/10.1186/s12951-017-0308-z
Solovev YV, Prilepskii AY, Krivoshapkina EF, Fakhardo AF, Bryushkova EA, Kalikina PA, Koshel EI, Vinogradov VV (2019) Sol-gel derived boehmite nanostructures is a versatile nanoplatform for biomedical applications. Sci Rep 9:1–4. https://doi.org/10.1038/s41598-018-37589-1
Thakur K, Sharma G, Singh B, Chhibber S, Katare OP (2019) Nano-engineered lipid-polymer hybrid nanoparticles of fusidic acid: an investigative study on dermatokinetics profile and MRSA-infected burn wound model. Drug Deliv Transl Res 9:748–763. https://doi.org/10.1007/s13346-019-00616-3
Wang K, Qi Z, Pan S, Zheng S, Wang H, Chang Y, Li H, Xue P, Yang X, Fu C (2020) Preparation, characterization and evaluation of a new film based on chitosan, arginine and gold nanoparticle derivatives for wound-healing efficacy. RSC Adv 10:20886–20899. https://doi.org/10.1039/D0RA03704D
Wilkinson HN, Hardman MJ (2020) Wound healing: cellular mechanisms and pathological outcomes. Open Biol 10:1748–1763. https://doi.org/10.1098/rsob.200223
Yazdanian M, Rostamzadeh P, Rahbar M, Alam M, Abbasi K, Tahmasebi E, Tebyaniyan H, Ranjbar R, Seifalian A, Yazdanian A (2022) The potential application of green-synthesized metal nanoparticles in dentistry: a comprehensive review. Bioinorg Chem Appl. https://doi.org/10.1155/2022/2311910
Yosefzon Y, Soteriou D, Feldman A, Kostic L, Koren E, Brown S, Ankawa R, Sedov E, Glaser F, Fuchs Y (2018) Caspase-3 regulates YAP-dependent cell proliferation and organ size. Mol Cell 17:573–587. https://doi.org/10.1016/j.molcel.2018.04.019
Zhou J, Li LU, Fang LI, Xie H, Yao W, Zhou X, Xiong Z, Wang LI, Li Z, Luo F (2016) Quercetin reduces cyclin D1 activity and induces G1 phase arrest in HepG2 cells. Oncol Lett 12:516–522. https://doi.org/10.3892/ol.2016.4639
Acknowledgements
This project was extracted from PhD thesis of Hilda Parastar. Moreover, we acknowledge Sara laboratory and Miss. Forough Janani for laboratory and histopathology analysis and the Board of Researcheditor.ir, for providing the best scientific and editorial services.
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This experiment was based on protocols suggested by Ethical Committee of Islamic Azad University of Zanjan University (No. 184706/December 10, 2020).
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Parastar, H., Farahpour, M.R., Shokri, R. et al. Acceleration in healing of infected full-thickness wound with novel antibacterial γ-AlOOH-based nanocomposites. Prog Biomater 12, 123–136 (2023). https://doi.org/10.1007/s40204-022-00216-4
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DOI: https://doi.org/10.1007/s40204-022-00216-4