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Green Synthesis-Mediated Silver Nanoparticles Based Biocomposite Films for Wound Healing Application

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Abstract

Wound care is a clinical challenge due to the susceptibility of the wound to multidrug-resistant bacterial infections. We report a multifunctional biocomposite of chitosan and gelatin (ratio = 1:1) based on silver nanoparticles (Ag NPs). The Ag NPs were selected due to their antimicrobial nature and were synthesised from an eco-friendly route using Ocimum sanctum (tulsi). The size of particles was 28.95 ± 7.74 nm in Transmission electron microscopy micrographs. Different concentrations of Ag NPs (0, 1, 2%, w/v) were used to prepare nano-biocomposite films (CG, CG1 and CG2) through the physical blending process followed by the solvent evaporation method. The physical properties of films, such as thickness and weight, were 0.10–0.12 mm and 0.93–1.55 g, respectively. Scanning electron microscopy and thermal gravimetric analysis studied the films' morphology and thermal stability. The Fourier transform infrared spectra affirmed the bond between silver and chitosan–gelatin. Nano-biocomposites showed high water uptake (300%) due to Ag NPs. Further, in vitro studies revealed antibacterial activity (Escherichia coli) with good in vitro biocompatibility and wound healing potential in the L929 mouse fibroblast cell line. Thus, the silver nanoparticle-biocomposite could be an effective topical dressing for biomedical applications.

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References

  1. M.E. Okur, I.D. Karantas, Z. Şenyiğit, N. Üstündağ Okur, P.I. Siafaka, Asian J. Pharm. Sci. 15, 661 (2020)

    Article  PubMed  PubMed Central  Google Scholar 

  2. D.M. Suflet, I. Popescu, I.M. Pelin, D.L. Ichim, O.M. Daraba, M. Constantin, G. Fundueanu, Pharmaceutics 13, 1461 (2021)

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  3. R. Augustine, A. Hasan, J. Drug Deliv. Sci. Technol. 56, 101516 (2020)

    Article  CAS  Google Scholar 

  4. H. Bardania, R. Mahmoudi, H. Bagheri, Z. Salehpour, M.H. Fouani, B. Darabian, S.S. Khoramrooz, A. Mousavizadeh, M. Kowsari, S.E. Moosavifard, G. Christiansen, D. Javeshghani, M. Alipour, M. Akrami, Sci. Rep. 10, 1 (2020)

    Article  CAS  Google Scholar 

  5. X. Wang, J. Chang, C. Wu, Appl. Mater. Today 11, 308 (2018)

    Article  Google Scholar 

  6. W. Paul and C. P. Sharma, Adv. Wound Heal. Mater. (2015).

  7. S. Stratton, N.B. Shelke, K. Hoshino, S. Rudraiah, S.G. Kumbar, Bioact. Mater. 1, 93 (2016)

    Article  PubMed  PubMed Central  Google Scholar 

  8. H.T. Peng, Mil. Med. Res. 7, 13 (2020)

    PubMed  PubMed Central  Google Scholar 

  9. N. R. Kunio and M. A. Schreiber, in Consult. Hemost. Thromb., Third Edit (Elsevier, 2013), pp. 538–545.

  10. N. Nady, S.H. Kandil, Membranes (Basel). 8, 2 (2018)

    Article  PubMed Central  CAS  Google Scholar 

  11. S. Murali, S. Kumar, J. Koh, S. Seena, P. Singh, A. Ramalho, A.J.F.N. Sobral, Cellulose 26, 5347 (2019)

    Article  CAS  Google Scholar 

  12. M.A. Khan, M. Mujahid, Int. J. Biol. Macromol. 124, 138 (2019)

    Article  PubMed  CAS  Google Scholar 

  13. M. Bin Ahmad, J.J. Lim, K. Shameli, N.A. Ibrahim, M.Y. Tay, Molecules 16, 7237 (2011)

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  14. H. Li, F. Cheng, S. Gao, Z. Wu, L. Dong, S. Lin, Z. Luo, X. Li, J. Appl. Polym. Sci. 134, 45441 (2017)

    Article  CAS  Google Scholar 

  15. I.C. Carvalho, H.S. Mansur, Mater. Sci. Eng. C 78, 690 (2017)

    Article  CAS  Google Scholar 

  16. V.C. Nguyen, V.B. Nguyen, M.-F. Hsieh, Int. J. Polym. Sci. 2013, 1 (2013)

    Article  CAS  Google Scholar 

  17. X. Huang, Y. Zhang, X. Zhang, L. Xu, X. Chen, S. Wei, Mater. Sci. Eng. C 33, 4816 (2013)

    Article  CAS  Google Scholar 

  18. S. Pirtarighat, M. Ghannadnia, S. Baghshahi, J. Nanostructure Chem. 9, 1 (2019)

    Article  CAS  Google Scholar 

  19. M. Pandima Devi, Int. J. Pharma Bio Sci. 7, P38 (2016)

    Google Scholar 

  20. G. Sathishkumar, C. Gobinath, K. Karpagam, V. Hemamalini, K. Premkumar, S. Sivaramakrishnan, Colloids Surfaces B Biointerfaces 95, 235 (2012)

    Article  PubMed  CAS  Google Scholar 

  21. A.D. Russell, W.B. Hugo, Prog. Med. Chem. 31, 351–370 (1994)

    Article  PubMed  CAS  Google Scholar 

  22. N. Krithiga, A. Rajalakshmi, A. Jayachitra, J. Nanosci. 2015, 1 (2015)

    Article  Google Scholar 

  23. N.K. Rajendran, S.S.D. Kumar, N.N. Houreld, H. Abrahamse, J. Drug Deliv. Sci. Technol. 44, 421 (2018)

    Article  CAS  Google Scholar 

  24. S. Zafar, A. Zafar, Open Biotechnol. J. 13, 37 (2019)

    Article  CAS  Google Scholar 

  25. S.B. Aziz, G. Hussein, M.A. Brza, S.J. Mohammed, R.T. Abdulwahid, S. Raza Saeed, A. Hassanzadeh, Nanomaterials 9, 1557 (2019)

    Article  CAS  Google Scholar 

  26. A. Usmani, P.P. Dash, A. Mishra, Mater. Sci Adv. Compos. Mater. 2, 1 (2018)

    Article  Google Scholar 

  27. S. Ediyilyam, B. George, S.S. Shankar, T.T. Dennis, S. Wacławek, M. Černík, V.V.T. Padil, Polymers (Basel). 13, 1680 (2021)

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  28. S. Kumar, A. Shukla, P.P. Baul, A. Mitra, D. Halder, Food Packag. Shelf Life 16, 178 (2018)

    Article  Google Scholar 

  29. S.A. Bhoir, S.P. Chawla, Int. J. Nanosci. 16, 1650022 (2017)

    Article  CAS  Google Scholar 

  30. S. Paul, A. Jayan, C.S. Sasikumar, Asian Pacific J. Trop. Dis. 5, 975 (2015)

    Article  CAS  Google Scholar 

  31. J. Venkatesan, J.-Y. Lee, D.S. Kang, S. Anil, S.-K. Kim, M.S. Shim, D.G. Kim, Int. J. Biol. Macromol. 98, 515 (2017)

    Article  PubMed  CAS  Google Scholar 

  32. K. Paulkumar, G. Gnanajobitha, M. Vanaja, M. Pavunraj, G. Annadurai, Adv. Nat. Sci. Nanosci. Nanotechnol. 8, 035019 (2017)

    Article  CAS  Google Scholar 

  33. E. Parthiban, N. Manivannan, R. Ramanibai, N. Mathivanan, Biotechnol. Rep. 21, e00297 (2019)

    Article  Google Scholar 

  34. J. S. Moodley, S. B. N. Krishna, K. Pillay, Sershen, and P. Govender, Adv. Nat. Sci. Nanosci. Nanotechnol. 9, 015011 (2018).

  35. D. Bharathi, D.M. Josebin, S. Vasantharaj, V. Bhuvaneshwari, J. Nanostruct. Chem. 8, 83 (2018)

    Article  CAS  Google Scholar 

  36. K. Anandalakshmi, J. Venugobal, V. Ramasamy, Appl. Nanosci. 6, 399 (2016)

    Article  CAS  Google Scholar 

  37. K. Shameli, M. Ahmad, P. Shabanzadeh, A. Zamanian, P. Sangpour, Y. Abdollahi, Z. Mohsen, Int. J. Nanomedicine 7, 5603 (2012)

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  38. D. Parida, C. Bakkali-Hassani, E. Lebraud, C. Schatz, S. Grelier, D. Taton, J. Vignolle, Nanoscale 14, 4635 (2022)

    Article  PubMed  CAS  Google Scholar 

  39. D. Nguyen, J. Lee, K. Park, Y. Ching, X. Nguyen, V.H. Phan, T. Hoang Thi, Nanomaterials 10, 542 (2020)

    Article  PubMed Central  CAS  Google Scholar 

  40. O. Erdogan, M. Abbak, G.M. Demirbolat, F. Birtekocak, M. Aksel, S. Pasa, O. Cevik, PLoS ONE 14, e0216496 (2019)

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  41. G. Singhal, R. Bhavesh, K. Kasariya, A.R. Sharma, R.P. Singh, J. Nanoparticle Res. 13, 2981 (2011)

    Article  CAS  Google Scholar 

  42. Z. A. Ali, R. Yahya, S. D. Sekaran, and R. Puteh, Adv. Mater. Sci. Eng. 2016, (2016).

  43. S. Hasatsri, A. Pitiratanaworanat, S. Swangwit, C. Boochakul, C. Tragoonsupachai, Dermatol. Res. Pract. 2018, 1 (2018)

    Article  Google Scholar 

  44. R. Tandon, S. Gandhi, S. Khushu, R.P. Tripathi, J. Chitin Chitosan Sci. 2, 35 (2014)

    Article  Google Scholar 

  45. M. Uzun, S.C. Anand, T. Shah, J. Biomed. Eng. Technol. 1, 1 (2013)

    Google Scholar 

  46. D. Tripathi, A. Sharma, P. Tyagi, C.S. Beniwal, G. Mittal, A. Jamini, H. Singh, A. Tyagi, AAPS PharmSciTech 22, 1 (2021)

    Article  CAS  Google Scholar 

  47. R. Nazir, D. Parida, J. Borgstädt, S. Lehner, M. Jovic, D. Rentsch, E. Bülbül, A. Huch, S. Altenried, Q. Ren, P. Rupper, S. Annaheim, S. Gaan, Chem. Eng. J. 417, 128028 (2021)

    Article  CAS  Google Scholar 

  48. A.V. Fuchs, S. Ritz, S. Pütz, V. Mailänder, K. Landfester, U. Ziener, Biomater. Sci. 1, 470 (2013)

    Article  PubMed  CAS  Google Scholar 

  49. K. Nešporová, V. Pavlík, B. Šafránková, H. Vágnerová, P. Odráška, O. Žídek, N. Císařová, S. Skoroplyas, L. Kubala, V. Velebný, Sci. Rep. 10, 1 (2020)

    Article  CAS  Google Scholar 

  50. B. Boonkaew, P. Suwanpreuksa, L. Cuttle, P. M. Barber, and P. Supaphol, J. Appl. Polym. Sci. 131 (2014).

  51. M.O. Wang, J.M. Etheridge, J.A. Thompson, C.E. Vorwald, D. Dean, J.P. Fisher, Biomacromol 14, 1321 (2013)

    Article  CAS  Google Scholar 

  52. D. Zhang, Z. Hu, L. Zhang, S. Lu, F. Liang, S. Li, Materials (Basel). 13, 5038 (2020)

    Article  PubMed Central  CAS  Google Scholar 

  53. C.C. Liang, A.Y. Park, J.L. Guan, Nat. Protoc. 2, 329 (2007)

    Article  PubMed  CAS  Google Scholar 

  54. G. Yaşayan, G. Karaca, Z.P. Akgüner, A. Bal Öztürk, Int. J. Polym. Mater. Polym. Biomater. 70, 623 (2021)

    Article  CAS  Google Scholar 

  55. K. Ravishankar, M. Venkatesan, R.P. Desingh, A. Mahalingam, B. Sadhasivam, R. Subramaniyam, R. Dhamodharan, Mater. Sci. Eng. C 102, 447 (2019)

    Article  CAS  Google Scholar 

  56. A. Suarez-Arnedo, F. Torres-Figueroa, C. Clavijo, P. Arbeláez, J.C. Cruz, C. Muñoz-Camargo, PLoS ONE 15, e0232565 (2020)

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  57. M. Behravan, A. Hossein Panahi, A. Naghizadeh, M. Ziaee, R. Mahdavi, A. Mirzapour, Int. J. Biol. Macromol. 124, 148 (2019)

    Article  PubMed  CAS  Google Scholar 

  58. B. Adebayo Tayo, A. Salaam, A. Ajibade, Heliyon 5, e02502 (2019)

    Article  PubMed  PubMed Central  Google Scholar 

  59. M.M.H. Khalil, E.H. Ismail, K.Z. El-Baghdady, D. Mohamed, Arab. J. Chem. 7, 1131 (2014)

    Article  CAS  Google Scholar 

  60. Annu, A. Ali, and S. Ahmed, Handb. Ecomater. 1 (2018).

  61. S. Ahmed, Saifullah, M. Ahmad, B. L. Swami, and S. Ikram, J. Radiat. Res. Appl. Sci. 9, 1 (2016).

  62. J. Singh, T. Dutta, K.H. Kim, M. Rawat, P. Samddar, P. Kumar, J. Nanobiotechnology 16, 1 (2018)

    Article  CAS  Google Scholar 

  63. M. Sundrarajan, S. Ambika, K. Bharathi, Adv. Powder Technol. 26, 1294 (2015)

    Article  CAS  Google Scholar 

  64. S. Patel, S. Srivastava, M.R. Singh, D. Singh, Int. J. Biol. Macromol. 107, 1888 (2018)

    Article  PubMed  CAS  Google Scholar 

  65. W. Cao, J. Yan, C. Liu, J. Zhang, H. Wang, X. Gao, H. Yan, B. Niu, W. Li, Carbohydr. Polym. 247, 116643 (2020)

    Article  PubMed  CAS  Google Scholar 

  66. S. Ahmed, S. Ikram, J. Photochem. Photobiol. B Biol. 163, 115 (2016)

    Article  CAS  Google Scholar 

  67. R.A. Mauricio-Sánchez, R. Salazar, J.G. Luna-Bárcenas, A. Mendoza-Galván, Vib. Spectrosc. 94, 1 (2018)

    Article  CAS  Google Scholar 

  68. L. Ding, X. Shan, X. Zhao, H. Zha, X. Chen, J. Wang, C. Cai, X. Wang, G. Li, J. Hao, G. Yu, Carbohydr. Polym. 157, 1538 (2017)

    Article  PubMed  CAS  Google Scholar 

  69. V.Q. Nguyen, M. Ishihara, J. Kinoda, H. Hattori, S. Nakamura, T. Ono, Y. Miyahira, T. Matsui, J. Nanobiotechnology 12, 1 (2014)

    Article  CAS  Google Scholar 

  70. A. Regiel-Futyra, M. Kus-Liśkiewicz, V. Sebastian, S. Irusta, M. Arruebo, A. Kyzioł, G. Stochel, RSC Adv. 7, 52398 (2017)

    Article  PubMed  CAS  Google Scholar 

  71. S.R. ur Rehman, R. Augustine, A.A. Zahid, R. Ahmed, M. Tariq, A. Hasan, Int. J. Nanomedicine 14, 9603 (2019)

    Article  Google Scholar 

  72. V. Holubnycha, O. Kalinkevich, O. Ivashchenko, M. Pogorielov, Nanoscale Res. Lett. 13, 1 (2018)

    Article  CAS  Google Scholar 

  73. C. Ramteke, T. Chakrabarti, B.K. Sarangi, R. Pandey, J. Chem. 2013, 1 (2013)

    Article  CAS  Google Scholar 

  74. S. Jain, M.S. Mehata, Sci. Rep. 7, 1 (2017)

    Article  CAS  Google Scholar 

  75. Y. Rout, J. Microbiol. Antimicrob. 4, 103 (2012)

    Article  CAS  Google Scholar 

  76. A.M. Díez-Pascual, A.L. Díez-Vicente, Biomacromol 16, 2631 (2015)

    Article  CAS  Google Scholar 

  77. S. Shetty, S. Udupa, L. Udupa, Evidence-Based Complement. Altern. Med. 5, 95 (2008)

    Article  Google Scholar 

  78. R. Barreto, R. Albuquerque-Júnior, A. Araújo, J. Almeida, M. Santos, A. Barreto, J. DeSantana, P. Siqueira-Lima, J. Quintans, L. Quintans-Júnior, Molecules 19, 846 (2014)

    Article  PubMed  PubMed Central  CAS  Google Scholar 

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Acknowledgements

The authors are grateful to the Director, Institute of Nuclear Medicine and Allied Sciences, Defence Research and Development Organisation, Delhi, India and Indian Institute of Technology, Delhi, and India for financial support. Isha Gupta would like to acknowledge the Union Grant Commission, New Delhi, India, for providing fellowship (Fellowship No. 114562). Abhishek Kumar is a fellowship recipient from the Council of Scientific and Industrial Research, New Delhi, India.

Funding

Funding was provided by University Grants Commission (Grant No.: 114562) and Council of Scientific and Industrial Research, India

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Authors and Affiliations

  1. Institute of Nuclear Medicine and Allied Sciences (INMAS), Defence Research and Development Organization (DRDO), Delhi, India

    Isha Gupta, Abhishek Kumar, Anant Narayan Bhatt & Sonia Gandhi

  2. Department of Chemistry, Indian Institute of Technology Delhi (IITD), New Delhi, India

    Isha Gupta & Sameer Sapra

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  1. Isha Gupta
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Contributions

IG: Conceptualisation, data curation, formal analysis, writing, methodology, original draft preparation, review and editing. AK: Data curation, analysis. ANB: Resources, review and editing. SS: Resources, supervision, review and editing. SG: Conceptualisation, funding acquisition, review and editing, resources, supervision, project administration.

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Correspondence to Sonia Gandhi.

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Gupta, I., Kumar, A., Bhatt, A.N. et al. Green Synthesis-Mediated Silver Nanoparticles Based Biocomposite Films for Wound Healing Application. J Inorg Organomet Polym 32, 2994–3011 (2022). https://doi.org/10.1007/s10904-022-02333-w

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