Abstract
The influence of genotype on the conversion of pollen to bee bread and on the protein titers of bees feeding on it was examined using European and Africanized honey bees (EHB and AHB). Bee bread was more acidic than the pollen, and that made by EHB was slightly more acidic than AHB. Protein concentration in bee bread was similar for both subspecies and lower than in the pollen. In general, amino acid concentrations were higher in bee bread compared with pollen. The only exception was tryptophan. Concentrations of most amino acids in bee bread made by either EHB or AHB were similar. Both subspecies consumed more bee bread made by AHB than EHB. EHB and AHB consumed similar amounts of each type of bee bread, but protein concentrations in AHB were higher than in EHB. Differences in protein acquisition between AHB and EHB might reflect environmental adaptations related to the geographic region where each evolved and could contribute to the successful establishment of AHB populations in the New World.
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1 Introduction
Nutrition plays a fundamental role in the health and vigor of honey bee colonies and in their establishment as populations. Nutrients from food provide energy and the biochemical components needed for brood rearing, thermoregulation, foraging and immune response. For honey bee colonies, the nutrients needed to grow colony populations and maintain their health come from nectar and pollen. Nectar provides carbohydrates and pollen supplies the remaining dietary requirements such as protein, lipids, vitamins, and minerals (see Brodschneider and Crailsheim 2010).
Subspecies of honeybees can differ in nutritionally based colony-level parameters such as worker longevity, brood rearing, and mechanisms of social immunity (Spivak 1992; Schneider and McNally 1993; Becerra-Guzman et al. 2005; Saltykova et al. 2005; Decanini et al. 2007). These differences might be linked to how food, particularly pollen, is processed by the colony and digested in individuals. Pollen is stored in comb cells and through microbially mediated lactic acid fermentation (Gilliam 1979a, b; Gilliam et al. 1989) is chemically changed (Loper et al. 1980). The fermented pollen is called bee bread. There might be genotypic differences among subspecies in their ability to acquire nutrients from the pollen they collect and store and this could affect colony growth and survival. Genotypic effects on food processing and nutrient acquisition have been documented in other organisms (e.g., Khachatryan et al. 2008), causing some individuals to obtain more nutrients and calories than others while consuming the same foods (Turnbaugh et al. 2006; Ley et al. 2005, 2006). Accumulating evidence suggests that the gut microbiota plays an important role in nutrient acquisition from food (Krajmalnik-Brown et al. 2012), and that host genotype can have a tremendous effect on the diversity and population structure of the microbiota (Spor et al 2011). In honey bees, how genotype affects nutrient acquisition from pollen either through the fermentation process or digestion in individuals is unknown.
Previous studies on the composition and nutritive value of bee bread were done with European honey bees (EHB) (e.g., Standifer et al. 1980; Loper et al. 1980; Human and Nicolson 2006). However, there may be differences in the composition and consumption of bee bread made by bees of different subspecies even when they feed on the same pollen. We addressed this question by presenting the same pollen to Africanized honey bees (AHB) and EHB and then comparing the consumption and the protein and amino acid composition of the bee bread produced by each subspecies. We chose these subspecies because EHB and AHB have distinct behavioral and physiological differences that might be related to food processing and nutrient acquisition. Some of the most notable are that AHB collect and consume more pollen than EHB and seem to convert it more readily into brood (Winston 1992). AHB colonies have higher swarming rates than EHB and abscond when food resources become limited (Woyke 1976, 1977; Winston et al. 1979; Schneider and McNally 1992a; Hepburn et al. 1999). Absconding is rare in EHB. AHB also have higher metabolic rates than EHB (Harrison et al. 2006).
The nutritional basis for the colony-level differences between EHB and AHB might be related to the rate of pollen collection and also to its nutrient content (e.g., amino acids and protein) after it is converted to bee bread. Bee bread consumption and the resulting protein acquisition also might play a role in the colony-level differences between EHB and AHB. In this study, we fed bee bread made by EHB and AHB to both subspecies of bees. Thus, we could determine if bees acquire protein from bee bread at rates that are distinctive to their subspecies or to the source of the bee bread.
2 Methods and materials
All EHB used in the study were Italian bee (Apis mellifera ligustica) colonies headed by commercially produced and mated European queens (Koehnen and Sons Inc., Glenn, California). The AHB were captured from swarms in Tucson, AZ, USA. Southern Arizona has an established feral population of Africanized bees that originated from the northward expansion of Africanized populations following the introduction of A. mellifera scutellata into Brazil (Fewell and Bertram 2002; Schneider et al. 2004a; Rabe et al. 2005). Africanization of the bees used in this study was confirmed using mitochondrial DNA (Nielsen et al. 2000) and morphometric analyses (Rinderer et al. 1993). All source colonies for this study were maintained in apiaries at the Carl Hayden Bee Research Center in Tucson, AZ, USA. Tucson is located in the Sonoran Desert and has an arid climate with annual precipitation usually less than 40 cm.
2.1 Obtaining frames of bee bread from Africanized and European colonies
Five colonies each of EHB and AHB were used to make bee bread. Each EHB or AHB colony was comprised of five frames of adult bees and unsealed brood (about 3,500–4,000 adult bees per colony). The colonies were placed in an enclosed flight area (EFA) at the Carl Hayden Bee Research Center in Tucson, AZ, USA. The EFA is separated into 10 sections. Bees cannot forage outside the enclosure or cross between sections. One colony (either EHB or AHB) was placed in each of the 10 sections so that five sections contained EHB and five contained AHB. The colonies had no pollen stores when we established them in the enclosure but did contain open brood and some nectar and honey. Empty frames were placed in the colonies to provide space for the bees to store pollen. These colonies were used only to make bee bread with the pollen we provided.
The pollen we provided was a polyfloral mixture collected by colonies in apiaries in the Sonoran Desert in Arizona, USA. The pollen was collected less than 6 months before the study and kept frozen until fed to the bees. The pollen was fed in powdered form. Prior to feeding the pollen to the bees, it was analyzed for pesticides and fungicides by the USDA-AMS National Science Laboratory in Gastonia, NC, USA. The pollen was free of contaminants (data not shown). We took six samples of the pollen for protein and amino acid analysis. All pollen samples were kept at −20 °C until analyzed.
Pollen was provided to the colonies on trays in each section of the EFA. The bees collected the pollen on their hind legs as corbicular loads and stored it in cells. The bee bread was produced during a 3-week period and was used to feed a separate group of worker bees in cages. Bee bread from the European colonies is hereafter referred to as European bee bread (EBB) and from Africanized colonies as Africanized bee bread (ABB).
2.2 Feeding bees in cages
Cages containing 100 newly emerged EHB or AHB worker bees were established for each of the following treatment combinations: AHB fed ABB, EHB fed ABB, AHB fed EBB, and EHB fed EBB. There were six cages for each treatment combination. The bees for the cages came from frames of sealed worker brood that were removed from Africanized and European colonies and placed in separate emergence boxes in a climate-controlled environmental room at 32–34 °C and 70 % humidity. When the workers emerged and were about 24 h old, they were transferred to the Plexiglas bioassay cages (dimensions = 11.5 × 7.5 × 16.5 cm). One hundred workers were placed in each cage. The cages contained a section of comb with either EBB or ABB selected at random from the frames of the colonies in the EFA. Each section of comb was comprised of 24–30 cells filled to approximately the same level with bee bread. The exact number of cells in each cage was recorded prior to adding the bees. The bees in the cages also were supplied with vials of water and a 50 % honey and water solution. The honey and water vials were refilled daily. The cages with the bees remained in the environmental room at 32–34 °C and 70 % humidity for 11 days.
2.3 Sampling worker bees and bee bread and estimating consumption
Ten EHB and AHB workers were sampled from the newly emerged bees prior to placing them in the cages. These bees are referred to as day 0 bees. An additional 10 bees were removed from each cage 4, 7, and 11 days after feeding on the bee bread. The live bees were placed in individual Eppendorf tubes and set on ice packs. A subsample of four bees from the 10 collected was used for analysis of hemolymph protein concentration.
After sampling bees on day 11, the number of comb cells that still contained bee bread was counted for each cage and used as a relative measure of bee bread consumption. We chose this method because the bees stored some of the honey we fed them in the empty cells on the comb thus making changes in weight of the comb before and after feeding inaccurate. The remaining bee bread from the cells in each cage was removed and stored in separate Eppendorf tubes according to cage at −70 °C until analyzed for pH, soluble protein concentration, and amino acid content.
2.4 Estimating pH of pollen and bee bread
The pH of the pollen used to make bee bread was estimated using six random samples of the pollen used for feeding the colonies in the EFA. A sample of bee bread from each cage that remained after the 11-day feeding period was used to estimate the pH of bee bread. We estimated the pH using a 0.3-g sample of pollen or bee bread placed in a 1.5-ml centrifuge tube (Eppendorf North America, Westbury, NY) and dissolved in 300 μl of distilled water. The pH of the mixture was measured with a waterproof double junction pH spear (Thermo Fisher Scientific) with an accuracy of +0.01.
2.5 Protein analysis
We analyzed soluble protein concentrations in: the pollen used to make bee bread, EBB and ABB, and in the hemolymph of bees that were 0, 4, 7, and 11 days old. Six samples of the pollen and a sample of EBB and ABB from each cage were collected and kept at −20 °C until analyzed. The bees were placed in separate 1.5 ml tubes and kept refrigerated until their hemolymph was collected 1–2 h later.
Pollen and bee bread were prepared for analysis of soluble protein concentration by mixing a 20-mg sample with 1,000 μl of phosphate buffer solution (PBS). The mixture was vortexed for 10 s and centrifuged at 8,500 rpm for 1 min. Ten-microliter samples of the supernatant were placed in wells of a 96-well flat bottom EIA/RIA polystyrene plate. Each sample was replicated in three wells.
Hemolymph from bees was drawn by inserting a 20-μL capillary tube (that had been heated and pulled to a needle-sharp point) into the right lateral portion of the thorax near the point of attachment of the wings. Additional hemolymph, if needed, was collected by inserting the same tube into the membrane between the abdominal tergites. Approximately 1–5 μL of hemolymph was collected in the capillary tube. Hemolymph samples were stored at −20 °C until analysis. The samples were prepared for analysis of soluble protein concentration by adding 1 μL of sample to 9 μL of PBS.
Total soluble protein concentrations in pollen, bee bread, and hemolymph samples were estimated using a Quick Start Bradford Protein Assay Kit 2 (#500-0202, Bio-Rad Laboratories, Hercules, CA) (Sagili et al. 2005; Sagili and Pankiw 2007). Standard curves to estimate soluble protein concentration in the samples were prepared using bovine serum albumin. Protein absorbance was measured at 595 nm using a Biotek Synergy HT spectrophotometer.
2.6 Amino acid analysis
Amino acid analysis was conducted by MDx BioAnalytical Laboratory, Inc, (College Station, TX). A single 0.3-g sample of pollen fed to bees and two 0.3-g samples each of EBB and ABB were analyzed for amino acid concentrations. The bee bread samples were obtained by pooling individual samples taken from comb cells of each colony. All samples were processed and analyzed in duplicate by gas chromatography–mass spectrometry (GC/MS) following trimethylsilyl (TMS) derivatization (Gehrke and Leimer 1971). Concentrations of amino acids are expressed as microgram of each amino acid per gram of pollen or bee bread.
Amino acid concentrations were estimated using a 50-mg pollen or bee bread sample weighed into autosampler vials; 1 mL of H2O was added to the vial, along with 100 μL of 50 ng/μL of a four internal standard solution consisting of d4-alanine, d23-lauric acid, 13C6-glucose, and d39-arachidiac acid. The sample was capped and sonicated for 5 min. An HLB cartridge was conditioned by adding 1 mL of methanol, equilibrated by adding 1 mL of water followed by the addition of 1 mL of sample. The cartridge was then washed with 1 mL of 5.0 % MeOH/water and eluted with 1 mL of 80 % MeOH/H2O. The extract was placed into an autosampler vial and evaporated to dryness under a stream of nitrogen. Subsequently, the sample was reconstituted with 50 μL of pyridine and 100 μL of N,O-Bis(trimethylsilyl)trifluoroacetamide + trimethylchlorosilane (BSTFA + TMCS). The solution was capped and incubated at 70 °C for 30 min. The sample was allowed to cool and then transferred to a glass insert, placed into a clean autosampler vial, and capped and placed into an Agilent 5973N Mass Selective Detector interfaced to an Agilent 6930 gas chromatograph to analyze the samples both for volatile compounds and organic acids. A J&W Scientific DB-1701 column (30 m × 0.25 mm i.d.) with a 1.0-μm film thickness was used to separate the sugar and organic acids following TMS derivatization with BSTFA + TMCS. The column oven was held at 50 °C for 2 min, then programmed linearly to 290 °C at 5 °C/min and held for 7 min. The GC injector and GC/MS interface were set to 250 and 290 °C, respectively. Helium was used as a carrier for both analyses at a flow rate of 1.0 mL/min. The MS source temperature was set to 230 °C. The mass spectrometer was tuned and calibrated daily with perfluorotributylamine (PFTBA). A 1-μL injection of PFTBA was made and data were acquired in the full scan (35–700 amu) positive ion mode.
2.7 Data analysis
The pH of the pollen was compared with EBB and ABB fed to either EHB or AHB using a one-way analysis of variance. We also compared the pH of EBB and ABB when fed to EHB or AHB using a two-way analysis of variance with the type of bee bread (EBB or ABB) and subspecies feeding on it (EHB or AHB) as factors. Bee bread consumption was estimated as the ratio of cells without bee bread after 11 days/cells with bee bread at the start of the study. Comparisons of the ratios of the bee bread consumed were made among the treatment groups using two-way analysis of variance with the same factors as those used for comparisons of pH. The soluble protein concentration in pollen and bee bread was compared using a one-way analysis of variance followed by a Fisher's least significant difference test (LSD) multiple comparison test (Field 2005). The concentration of soluble protein in the hemolymph during the 11-day feeding period was compared among cages of the four treatment groups using a repeated measures analysis of variance followed by a Fisher's LSD test with subspecies making the bee bread and subspecies consuming it as between subject factors. A second repeated measures analysis was conducted comparing hemolymph protein levels on days 4, 7, and 11 using subspecies that made the bee bread and subspecies consuming it as between subject factors. A full factorial model was used that contained all factor main effects, all covariate main effects, and all factor-by-factor interactions. In both analyses, the concentrations of soluble protein measured in bees were summed for each cage and used as a single measurement.
3 Results
3.1 pH of pollen and bee bread
EHB and AHB foragers collected the pollen we provided in the EFA, packed it in their corbiculae, and stored it in cells in their colonies. The average pH of the pollen we provided to the bees was 4.7 + 0.009, and this was significantly higher than the ABB or EBB fed to either subspecies (F = 1,827.8, df = 4, 22, P < 0.0001). The pH of EBB was 3.92 ± 0.013 and ABB was 3.97 ± 0.014. A two-way analysis of variance comparing EBB and ABB fed to EHB or AHB indicated that the pH of bee bread was significantly affected by the subspecies of bee that made it (F = 15.43, df = 1, 23, P = 0.001) and the subspecies of bee to which it was fed (F = 5.04, df = 1, 23, P = 0.036). The interaction term (subspecies × ABB or EBB) was not significant (F = 1.97, df = 1, 23, P = 0.176).
3.2 Consumption of bee bread
The bees in the cages clustered on the comb containing the bee bread within minutes of putting them into the cage. In most cases, there were fewer comb cells with bee bread after the 11-day feeding interval than when the experiment began. However, one cage of each treatment did not have any cells where bee bread was completely consumed although there was evidence that bees had been feeding on the pollen and that some was removed from cells. We did not estimate bee bread consumption in these cages.
A two-way analysis of variance indicated that the amount of bee bread consumed was significantly affected by the subspecies that made it (F = 20.92, df = 1, 16, P < 0.0001) but not the subspecies consuming it (F = 0.54, df = 1, 16, P = 0.47). The interaction term was not significant (F = 0.45, df = 1, 16, P = 0.513). EHB and AHB consumed similar amounts when fed the same type of bee bread but consumed significantly more ABB than EBB (Figure 1).
3.3 Soluble protein concentration
The average concentration of soluble protein in the pollen was 837.5 + 51.7 μg/mL. The concentration in EBB fed to either EHB or AHB did not differ (t = 0.77, df = 7, P = 0.44) nor did the ABB fed to either subspecies (t = 0.32, df = 9, P = 0.753). Therefore, we combined the protein measurements for each type of bee bread. EBB had a soluble protein concentration of 567 + 26 μg/mL and the ABB was 577 + 30 μg/mL; these averages were not significantly different (t = 0.85, df = 21, P = 0.403). Pollen had a significantly higher concentration of soluble protein than EBB or ABB (F = 16.49; df = 2, 27, P < 0.0001).
The soluble protein concentration in the hemolymph of EHB and AHB on day 0 did not differ significantly (EHB = 1,125 + 200 μg/mL, AHB = 787 + 155 μg/mL; t = 1.41, df = 9, P = 0.192). A repeated measures analysis of variance of the average amount of soluble protein in the hemolymph during the 11-day interval indicated significant differences among the four treatment groups (F = 19.7, df = 3, 20, P < 0.001). There were significant effects of the subspecies consuming the bee bread (F = 12.1, df = 1, 20, P = 0.002), but not the source of bee bread (ABB or EBB) (F = 0.027, df = 1, 20, P = 0.87) or the interaction between them (F = 0.509, df = 1, 20, P = 0.48). Levels of soluble protein in AHB fed ABB were significantly higher than EHB fed either EBB (P = 0.018) or ABB (P = 0.008) (Figure 2). When bees were fed bee bread made by the other subspecies, protein levels in AHB fed EBB were significantly higher than EHB fed ABB (P = 0.029). There were no differences between subspecies when they were both fed EBB (P = 0.24). The age of the bees at the time of sampling also significantly affected soluble protein concentrations in the hemolymph. Levels were significantly lower in day 4 bees compared with day 7 (P < 0.0001) or 11 (P = 0.001). Day 7 and day 11 bees did not differ (P = 0.149).
3.4 Amino acid analysis of pollen and bee bread
Of the 10 amino acids that are essential for honey bees, all but histidine were detected in the pollen. Lysine and asparagine could not be accurately quantified in the bee bread due to interference from excess sugars. This also was the case for tyrosine in ABB. This amino acid was present in the pollen at a concentration of 198.6 μg/g and was quantified in one EBB sample (160.5 μg / g).
In most cases, amino acid concentrations measured in bee bread were higher than in the pollen (Figure 3). For example, concentrations of leucine and threonine were about 60 % higher in bee bread compared with pollen, and valine concentrations were about 25 % higher. Alanine, aspartic acid, and glutamine levels also were higher in bee bread than in pollen. Amino acid concentrations did not differ greatly between ABB and EBB with the exception of phenylalanine and cysteine. Phenylalanine levels were about twice as high in ABB compared with either EBB or pollen. Cysteine concentrations were lower in EBB compared with ABB or pollen. Tryptophan was the only amino acid present in higher concentrations in pollen than in EBB or ABB. Concentrations of proline in pollen and ABB were higher than in EBB.
4 Discussion
The most striking difference between the two types of bee bread was the amounts consumed and how this translated into soluble protein levels in the hemolymph. Bees of both subspecies consumed more ABB than EBB. Though both subspecies consumed similar amounts of ABB, AHB had higher hemolymph protein titers than EHB. Protein concentrations did not differ with respect to the type and amount of bee bread they consumed within either subspecies. There appears to be a plateau for hemolymph protein concentration in each subspecies that cannot be elevated by increasing food consumption and is set higher in AHB than EHB.
The bees were able to store the pollen we provided and reduce its pH during the storage and conversion to bee bread. Reductions in pH during the conversion of pollen to bee bread have been attributed to the activity of lactic acid bacteria that are added to the pollen from the honey stomach (Loper et al. 1980; Vasquez and Olofsson 2009). We found statistically significant differences in pH between EBB and ABB though the differences were slight and might not have affected pollen preservation or nutrient availability. However, the differences in pH between EBB and ABB suggest that the composition of microbial communities in ABB and EBB might not be the same especially with regard to those synthesizing acidic compounds from metabolic activities.
The consumption of bee bread in this experiment was an approximation because the only consumption that was counted was when cells were completely emptied of bee bread. We could have estimated total bee bread consumption by removing the bee bread from the cells and weighing it before and after the study period. However, we wanted to keep the bee bread in the cells so that the bees could feed on it as they would in a colony and continue processing it if necessary during the study period. We did not use the difference in weight of the comb sections before and after the study as an estimate of consumption because bees put the diluted honey we fed them in some cells and might also have added some to the bee bread. This would have made differences in comb weights inaccurate measurements of food consumption. For these reasons, we counted cells containing approximately equal amounts of bee bread before and after the feeding period. Though our measurements were qualitative, there was a striking difference between the two types of bee bread in the number of empty ABB cells we counted compared with EBB after 11 days.
One reason that more ABB than EBB was consumed might be that concentrations of phagostimulatory compounds differed between the two types of bee bread. One such compound might be proline. There was about 20 % more proline in ABB than EBB. Insects can detect proline in their food and can use it as an energy substrate to fuel flight and other high energy activities (Brosemer and Veerabhadrappa 1965; Crabtree and Newsholme 1970; Carter et al. 2006). Honey bees have a feeding preference for nectars with higher concentrations of this amino acid (Carter et al. 2006; Bertazzini et al. 2010). The preference for proline might also occur in bee bread and thus increase its consumption. Further studies are needed to confirm this and identify other differences between EBB and ABB that might affect consumption rates.
The nutritional value of pollen often is evaluated by protein concentrations and the presence and amount of essential amino acids (Bonvehi and Jorda 1997; Roulston and Cane 2000). Some pollens are classified as highly nutritious while others have marginal value (see Somerville 2005). However, these nutritional evaluations might not be accurate. In our study, protein concentrations were higher in pollen than in bee bread, but most amino acid concentrations were lower. Similar results were reported by Human and Nicolson (2006) in a study comparing fresh, bee-collected and bee-stored pollen from Aloe greatheadii var. davyana. One way that free amino acid levels can be elevated is from the action of specific proteolytic enzymes causing cleavage at sites of particular amino acids. Alternatively, amino acids can be synthesized de novo by microbes (Metges 2000). We also found that some amino acids concentrations were lower in bee bread than in the pollen. These reductions also might be due to microbial activity since some microorganisms use amino acids as carbon and energy sources. For example, concentrations of tryptophan were lower in EBB and ABB compared with that in pollen. The reduction could be due to the presence of indole bacteria previously reported by Chevtchik (1950) (cited in Gilliam 1979a) that use tryptophan as an energy source. Similarly, proline concentrations were lower in EBB than in the pollen or in ABB, and this could be due to bacteria with specific pathways to degrade amino acids (Collins et al. 1994; Fonknechten et al. 2010). Free amino acids also can be incorporated into proteins and this too would reduce their concentrations. The amino acid content of bee bread probably depends not only on the source of the pollen but perhaps the genotype of the bees processing it into bee bread. Thus, evaluating the nutritional value of pollen alone might not be as informative as considering its conversion to bee bread because nutrient concentrations can change. The role of genotype in this conversion should be further investigated.
Our findings that AHB have higher hemolymph protein levels than EHB are similar to those reported by Cappelari et al. (2009) using a different EHB subspecies: A. mellifera carnica. In that study, hemolymph protein concentrations were measured after feeding protein supplements or a mixture of bee bread (unknown source) and powdered sugar. In both studies, the AHB were from feral populations while the EHB were from domesticated lines. Protein levels in the hemolymph of AHB might differ from EHB, but there also might be differences within subspecies between managed and feral populations due to the selection pressures experienced by each. For example, managed colonies are fed with protein supplements when flowering plants are unavailable (Mattila and Otis 2006; DeGrandi-Hoffman et al. 2008), and when resources are abundant, nests are expanded by adding frames of drawn comb. Feral colonies must balance brood rearing and storage space with incoming resources. This might cause workers in feral colonies to store more resources in their own bodies than their counterparts in managed hives. Future studies should examine hemolymph protein in feral and managed EHB to determine if differences exist that might be due to domestication and management practices.
Our study might have uncovered some nutritionally based factors contributing to the immigration and successful establishment of AHB in areas with resident EHB populations. AHB collect more pollen than EHB (Winston 1992; Spivak 1992; Schneider et al. 2004a) and can attain higher hemolymph protein levels from it. This would enable colonies to rear more brood and have workers with extended adult life spans (Schultz et al. 1998; Toth et al. 2005; Toth and Robinson 2005). Together these factors will generate rapid colony growth and high rates of swarming. While some swarms establish new nests, others can invade EHB colonies (Danka et al. 1992; Schneider et al. 2004b). During an invasion, the resident queen is often replaced by the Africanized queen in the swarm causing the EHB colony to become Africanized. Our study indicates that when AHB feed on EBB, they can attain hemolymph protein levels that are similar to those achieved by feeding on ABB. Interestingly, those protein levels are attained while consuming less EBB than ABB. The ability to efficiently use the food stored in the invaded colony increases the chances of its survival and thus could be a factor in the persistence of colony invasion behavior throughout the regions where AHB have immigrated.
Differences in protein uptake between EHB and AHB seem to be a function of the bee's metabolism rather than nutrient content or consumption rate of the bee bread itself. Those differences might be from environmental adaptations unique to where EHB and AHB originated. European bees are adapted to temperate climates and AHB to the tropics. In temperate areas, EHB have predictable patterns of resource availability and store excess food in their hives for periods of dearth (e.g., winter) (Seeley 1985). AHB are tropically adapted and have seasonal cycles of rapid colony expansion followed by swarming when resources are abundant and absconding when resources dwindle (McNally and Schneider 1992). Prior to absconding, workers consume all the brood and food stores in the nest (Schneider and McNally 1992b). Storing high concentrations of protein in their hemolymph would enable workers to carry more nutrients with them and perhaps begin rearing brood in their new nest prior to accumulating pollen stores. Indeed, during the establishment of new nests after absconding, African bees primarily raise brood and store little food (McNally and Schneider 1992). Differences between the environments where EHB and AHB originated might have resulted in different set points (i.e., homeostatic thresholds) for hemolymph protein concentrations much as they might have for vitellogenin levels (Amdam et al. 2005). The genetic structure responsible for establishing the vitellogenin set point has been instrumental in the evolution of the long-lived European “winter bee.” Similar genetic structure might exist for establishing hemolymph protein levels in AHB to enable long range migration and nest reestablishment in the tropics. Future studies will be directed at determining if differences in hemolymph protein concentrations between EHB and AHB are due to genetic factors especially those regulating expression of proteolytic enzyme synthesis by the bees and/or their microbial symbionts (Anderson et al. 2011).
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Acknowledgments
The authors thank Mona Chambers, Geoffrey Hidalgo, and Maurissa Two Two for their technical assistance. We also thank Dr. Earl White for amino acid analysis, Dr. Roger Simonds for analysis of pollen for pesticides, and Drs. Stanley S. Schneider, Vanessa Corby-Harris, and Kirk Anderson for their reviews and discussion of earlier versions of the manuscript and many helpful suggestions.
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DeGrandi-Hoffman, G., Eckholm, B.J. & Huang, M.H. A comparison of bee bread made by Africanized and European honey bees (Apis mellifera) and its effects on hemolymph protein titers. Apidologie 44, 52–63 (2013). https://doi.org/10.1007/s13592-012-0154-9
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DOI: https://doi.org/10.1007/s13592-012-0154-9