Abstract
Results of experiments are presented which have revealed differences in expression of genes of antibacterial peptides (ABP) after administration of pathogen (bacterial preparation, bitoxybacillin) between honeybees of the middle-Russian and Caucasian races as well as their hybrids. Interracial variations of the level of ABP gene expression were studied in the bees at the early stage of protective reactions. It was shown that the reaction was realized more rapidly in bees after their immunization and that purebred bees had some advantages over hybrids in development of the protective response with participation of ABP.
Similar content being viewed by others
REFERENCES
Glupov, V.V., Some Aspects of Insect Immunity, Usp. Sovr. Biol., 1992, vol. 112, pp. 62–73.
Casteels, P., Ampe, C., Jacobs, F., Vaeck, M., and Temst, P., Apidacins: Antibacterial Peptides from Honeybees, EMBO J., 1989, vol. 8, pp. 2387–2391.
Casteels, P., Ampe, C., Riviere, L., et al., Isolation and Characterization of Abaecin, a Major Antibacterial Response Peptide in the Honeybee (Apis mellifera), Eur. J. Biochem., 1990, vol. 187, pp. 381–386.
Casteels, P., Ampe, C., Jacobs, F., and Tempst, P., Functional and Chemical Characterization of Hymenoptaecin, an Antibacterial Polypeptide That Is Infection-Inducible in the Honeybee (Apis mellifera), J. Biol. Chem., 1993, vol. 268, pp. 7044–7054.
Saltykova, E.S., Poskryakov, A.V., Nikolenko, A.G., and Khairullin, R.M., Increase in Adaptivity in Honeybee Using Chitooligosaccharides, Ekologicheskii imperativ sel'skogo khozaistva Respubliki Bashkorstan (Ecological Imperative of Agriculture in the Republic of Bashkortostan), Ufa, 1998, pp. 67–68.
Saltykova, E.S., Adaptive Action of Chitooligosaccharides on Apis mellifera, Cand. Sci. Dissertation, St. Petersburg, 2000.
Herrington, C. and Macgi, J., Molekulyarnaya klinicheskaya diagnostika. Metody (Molecular Clinical Diagnostics. Methods), Moscow, 1999.
Chomezynski, P. and Sacchi, N., Single-Step Method of RNA Isolation by Acid Guanidium Thio-Cyanate-Phenol-Chloroform Extraction, Anal. Biochem., 1987, vol. 162, pp. 156–159.
Sambrook, J., Fritsch, E.F., and Maniatis, T., Molecular Cloning, a Laboratory Manual, New York, Cold Spring Harbor Laboratory, 1989.
Lvov, A.V. and Nikolenko, A.G., Expression and Polymorphism of Antibacterial Peptides Genes of Honeybee Apis mellifera mellifera, Biodiversity and Dynamics of Ecosystems in North Eurasia, Novosibirsk, 2000, vol. 3, Part 2, pp. 253–255.
Boman, H.G. and Hulmark, D., Cell-Free Immunity in Insects, Ann. Rev. Microbiol., 1987, vol. 41, pp. 103–126.
Zyuman, B.V., Antibacterial Peptides in Unspecific Resistance of Honeybee, Dokl. RASKhN, 1993, no. 4, pp. 70–76.
Zyuman, B.V., Factors and Mechanisms of Unspecific Resistance of the Honeybee (Apis mellifera L.), Doctorate Sci. Dissertation, Moscow, 1991.
Dimarcq, J.L., Zachary, D., Hoffman, J.A., et al., Expression of the Two Major Inducible Antibacterial Peptides, Defensin and Diptericin, in Phormia terranovae, EMBO J., 1990, vol. 9, pp. 2507–2515.
Lemaitre, B., Reichart, J.M., and Hoffman, J.A., Drosophila Host Defense: Deferential Induction of Antimicrobial Peptide Genes after Infection by Various Classes of Microorganisms, Proc. Natl Acad. Sci. USA, 1997, vol. 94, pp. 14614–14619.
Author information
Authors and Affiliations
Additional information
__________
Translated from Zhurnal Evolyutsionnoi Biokhimii i Fiziologii, Vol. 41, No. 5, 2005, pp. 404–407.
Original Russian Text Copyright © 2005 by Saltykova, Lvov, Ben'kovskaya, Poskryakov, Nikolenko.
Rights and permissions
About this article
Cite this article
Saltykova, E.S., Lvov, A.V., Ben'kovskaya, G.V. et al. Interracial Differences in Expression of Genes of Antibacterial Peptides, Abaecin, Hymenoptaecin, and Defensin, in Bees Apis mellifera mellifera and Apis mellifera caucasica . J Evol Biochem Phys 41, 506–510 (2005). https://doi.org/10.1007/s10893-005-0089-0
Received:
Issue Date:
DOI: https://doi.org/10.1007/s10893-005-0089-0