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Insights into the relevance between bacterial endophytic communities and resistance of rice cultivars infected by Xanthomonas oryzae pv. oryzicola

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Abstract

Bacterial leaf streak (BLS) caused by Xanthomonas oryzae pv. oryzicola (Xoc), impacts the production of rice. However, several rice cultivars displayed resistance to Xoc in the field, but scarce information is available about the role of endophytic microbiota in disease resistance. In the present study, the endophytic bacterial communities of resistant and susceptible rice cultivars “CG2” and “IR24”, respectively, were analyzed using high throughput 16S rRNA gene amplified sequencing and culture dependent method was further used for bacterial isolation. A total of 452,716 high-quality sequences representing 132 distinct OTUs (Proteobacteria, Actinobacteria, Bacteroidetes, and Firmicutes) and 46 isolates of 16 genera were explored from rice leaves infected with Xoc. Community diversity of endophytic bacteria were higher in the leaves of the resistant cultivars compared to susceptible cultivars upon Xoc infection. Strikingly, this diversity might contribute to natural defense of the resistant cultivar against pathogen. Pantoea, which is pathogen antagonist, was frequently detected in two cultivars and higher abundance were recorded in resistant cultivars. Different abundance genus includes endophytic isolates with marked antagonistic activity to Xoc. The increased proportions of antagonistic bacteria, may contribute to resistance of rice cultivar against Xoc and the Pantoea genus was recruited by Xoc infection play a key role in suppressing the development of BLS disease in rice. Taken together, this work reveals the association between endophytic bacteria and BLS resistance in rice and identification of antagonism-Xoc bacterial communities in rice.

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Acknowledgements

We thank the Agricultural Institute of Dehong State for providing rice seeds and the Dr. Fenghuan Yang of Chinese Academy of Agricultural Sciences for technical support.

Funding

This research was funded by the National Natural Science Foundation of China (32060601) and the National Key R&D Program of China (2019YFD1002000) and the Yunnan Ten Thousand Talents Plan leading talents of industrial technology project (YNWR-CYJS-2019-046). PhD research startup foundation of Chuxiong Normal University, China (BSQD2105).

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  1. Jun Yang and Zhenlin Dai contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, Yunnan, China

    Jun Yang, Zhenlin Dai, Xiaoyan Wan, Shahzad Munir, Xing Wang, Lanfang Wei & Guanghai Ji

  2. Academy of Science and Technology, Chuxiong Normal University, Chuxiong, 675000, Yunnan, China

    Jun Yang

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  1. Jun Yang
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Contributions

GHJ and LFW conceived and designed the experiments. JY, XYW, and XW performed the experiments. JY, and ZLD collect and analyzed the data. JY, and SM wrote the manuscript.

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Correspondence to Lanfang Wei or Guanghai Ji.

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Yang, J., Dai, Z., Wan, X. et al. Insights into the relevance between bacterial endophytic communities and resistance of rice cultivars infected by Xanthomonas oryzae pv. oryzicola. 3 Biotech 11, 434 (2021). https://doi.org/10.1007/s13205-021-02979-2

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