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Antifungal and Biocontrol Evaluation of Four Lysobacter Strains Against Clubroot Disease

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Abstract

The effect of crude extract (Ce), seed coating agent (SCA) and whole bacterial broth culture (WBC) of Lysobacter strains was evaluated against the causal agent of clubroot formation in Cruciferous vegetables. The ability of four Lysobacter strains (L. antibioticus 6-B-1, L. antibioticus 6-T-4, L. antibioticus 13-B-1 and L. capsici ZST1-2) inhibited Plasmodiophora brassicae of resting spores and disease. Application of WBC of four Lysobacter strains inhibited clubroot disease, indicating that the disease suppression was due to antifungal compounds produced by the biocontrol bacterium in the culture. Development of clubroot on Chinese cabbage was inhibited when the WBC and SCA were applied before P. brassicae inoculation. Crude extract (Ce) of culture filtrate was effective in arresting the germination of resting spores of P. brassicae on slides. However, Lysobacter strains differed in their biocontrol effects, the strain L. capsci ZST1-2 recorded a high level of disease limiting effect.

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Acknowledgements

This study is supported financially by the National Natural Foundation of China (31460458). The Key Project of the New Product Development Plan of Yunnan Province (2016ZF001). Joint Project for Agricultural basic Research of Science and Technology Department of Yunnan Province (2017FG001-005).

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Authors and Affiliations

  1. Key Laboratory of Agriculture Biodiversity for Plant Disease Management Under the Ministry of Education, Yunnan Agricultural University, Kunming, 650201, People’s Republic of China

    Lina Fu, Hanmei Li, Lanfang Wei, Jun Yang, Qi Liu, Yating Wang, Xing Wang & Guanghai Ji

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  1. Lina Fu
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Correspondence to Guanghai Ji.

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Fu, L., Li, H., Wei, L. et al. Antifungal and Biocontrol Evaluation of Four Lysobacter Strains Against Clubroot Disease. Indian J Microbiol 58, 353–359 (2018). https://doi.org/10.1007/s12088-018-0716-2

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