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Composition and diversity of endophytic bacterial community in seeds of super hybrid rice ‘Shenliangyou 5814’ (Oryza sativa L.) and its parental lines

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Abstract

Super hybrid rice is one of the important research achievements in the field of rice super-high yield breeding in China. Compared with general high-yield rice, the yield potential of super hybrid rice is relatively high, and the average yields per hectare can exceed 10.5 t. This study aimed to study the endophytic bacterial community among a super hybrid rice combination and its parental lines and to reveal the potential relationship and association of endophytic bacteria between rice genotypes and their genetic relevance. In this research, through high-throughput sequencing based on Illumina Hiseq 2500 platform, the seeds of super hybrid rice variety ‘Shenliangyou 5814’ (sample C) and its parental lines ‘Y58S’ (sample M) and ‘C4114’ (sample F) independently cultivated by China were used as plant materials to study their endophytic bacterial structure and diversity. The number of OTUs for sample M, F and C was 327, 288 and 283 respectively, and among them 218 endophytic OTUs coexisted in the 3 samples. Pantoea (18.6–31.1%) was the first dominant groups shared in all three samples, and other dominant shared groups belonged to Methylobacterium (4.48%~17.65%), Sphingomonas (4.0%~11.4%), Rhizobium (5.69–8.78%), Microbacterium (3.85%~9.41%) and Pseudomonas (4.13%~5.68%). Although Principal Coordinates Analysis (PCoA) and Non-metric Multidimensional Scaling (NMDS) analysis showed that there were obvious differences in endophytic bacterial community composition and structure among the 3 samples, the dominant endophytic bacterial genus sample C and its parental lines remained consistency. This study would provide scientific clues for the future research on the vertical transmission of endophytes between rice generations.

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Acknowledgements

This work was supported by the Fundamental Research Funds for the Central Universities (No. FRF-TP-18-012A1) and the Open Research Fund of State Key Laboratory of Hybrid Rice (Hunan Hybrid Rice Research Center) (No. 2017KF04). We also thank Dr. Zhengqiu Cai at Seres Therapeutics (USA) for assistance with English language and grammatical editing of the manuscript.

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Authors and Affiliations

  1. College of Chemistry and Biological Engineering, University of Science and Technology Beijing, Beijing, 100083, China

    Yang Liu, Fuzhen Yang, Miao Li & Hai Yan

  2. National Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha, 410125, China

    Ni Li & Weiping Wang

  3. Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Xiaoxia Zhang

  4. Nanjing Puwikon, Co., Ltd, Nanjing, 210012, China

    Pengpeng Xu

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  1. Yang Liu
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Liu, Y., Xu, P., Yang, F. et al. Composition and diversity of endophytic bacterial community in seeds of super hybrid rice ‘Shenliangyou 5814’ (Oryza sativa L.) and its parental lines. Plant Growth Regul 87, 257–266 (2019). https://doi.org/10.1007/s10725-018-0467-4

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