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Utilization of oil palm decanter cake for valuable laccase and manganese peroxidase enzyme production from a novel white-rot fungus, Pseudolagarobasidium sp. PP17-33

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Abstract

Oil palm decanter cake (OPDC) in the current study was converted to valuable products as laccase and manganese peroxidase (MnP) by an undescribed strain of the white-rot fungus, Pseudolagarobasidium sp. PP17-33. The optimization to enhance the production of enzymes through solid-state fermentation was performed using Plackett–Burman design and response surface methodology. The highest observed laccase was 5.841 U/gds and observed MnP was 5.156 U/gds, which enhanced yield by 2.59-fold and 1.94-fold from the non-optimization. The optimized medium (mg/g of OPDC) consisted of 0.852 mg CuSO4·5H2O, 13.512 mg glucose, 2 mg yeast extract, 0.2 mg KH2PO4, 1.5 mg MgSO4·7H2O, 0.01 mg FeSO4·7H2O, 0.15 mg MnSO4·H2O, 0.01 mg ZnSO4·7H2O and 0.3 mg Tween 80 (pH 5.0) when incubated at 30 °C for 7 days. The most significant variables of laccase and MnP productions were CuSO4·5H2O and glucose concentrations. This study is the first to report on the production of ligninolytic enzymes from OPDC waste using white-rot fungi. In addition, five different white-rot fungi, Coriolopsis aspera, C. retropicta, Dentipellis parmastoi, Nigroporus vinosus and Tyromyces xuchilensis, are newly observed producers of ligninolytic enzymes in Thailand. The results obtained from this study are significant not only for agro-industrial waste management but also for value-added enzyme production.

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References

  • Adak A, Tiwari R, Singh S, Sharma S, Nain L (2016) Laccase production by a novel white-rot fungus Pseudolagarobasidium acaciicola LA1 through fermentation of Parthenium biomass and its application in dyes decolorization. Waste Biomass Valor 7(6):1427–1435. https://doi.org/10.1007/s12649-016-9550-0

    Article  CAS  Google Scholar 

  • Adejoye OD, Fasidi IO (2010) Effect of cultural conditions on biomass and laccase production in submerged medium by Schizophyllum commune (FR.), a Nigerian edible mushroom. Electron J Environ Agric Food Chem 9:600–609

    CAS  Google Scholar 

  • Ajayi OO, Femi-Ola TO (2019) Evaluation of lignocellulosic enzymes profile of Pleurotus sajor-caju grown on selected agro-industrial wastes. Am J Microbiol Res 7(1):1–11

    Google Scholar 

  • Akpinar M, Urek RO (2017) Induction of fungal laccase production under solid state bioprocessing of new agroindustrial waste and its application on dye decolorization. 3 Biotech 7:98. https://doi.org/10.1007/s13205-017-0742-5

    Article  PubMed  PubMed Central  Google Scholar 

  • Asgher M, Iqbal HMN (2011) Characterization of a novel manganese peroxidase purified from solid state culture of Trametes versicolor IBL-04. BioResources 6(4):1–14

    Google Scholar 

  • Bodke PM, Senthilarasu G, Raghukumar S (2012) Screening diverse fungi for laccases of varying properties. Indian J Microbiol 52(2):247–250

    Article  CAS  Google Scholar 

  • Cupul WC, Abarca GH, Carrera DM, Vazquez RR (2014) Enhancement of ligninolytic enzyme activities in a Trametes maxima–Paecilomyces carneus co-culture: key factors revealed after screening using a Plackett-Burman experimental design. Electron J Biotechnol 17(3):114–121

    Article  CAS  Google Scholar 

  • Fonseca MI, Shimizu E, Zapata PD, Villalba LL (2010) Copper inducing effect on laccase production of white rot fungi native from Misiones (Argentina). Enzyme Microb Technol 46(6):534–539

    Article  CAS  Google Scholar 

  • Ghebreslasie Z, Premjet D, Permjet S (2016) Screening of fungi producing ligninolytic enzymes. KKU Res J 21(2):200–209

    Google Scholar 

  • Hawksworth DL (2001) The magnitude of fungal diversity: the 1.5 million species estimate revisited. Mycol Res 105(12):1422–1432

    Article  Google Scholar 

  • Kalmis E, Azbar N, Kalyoncu F (2007) Agar plate screening for textile dye decolorisation by white rot fungi Pleurotus species (Pleurotus cornucopiae var. citrinopileatus, P. djamor, P. eryngii, P. ostreatus and P. sajor-caju). Fresen Environ Bull 16:1309–1314

    CAS  Google Scholar 

  • Kaosol T, Sohgrathok N (2012) Enhancement of biogas production potential for anaerobic co-digestion of wastewater using decanter cake. Am J Agric Biol Sci 7(4):494–502

    Article  CAS  Google Scholar 

  • Kinnunen AJ, Maijala PM, Järvinen PP, Hatakka AI (2017) Improved efficiency in screening for lignin-modifying peroxidases and laccases of basidiomycetes. Curr Biotechnol 6(2):105–115

    Article  CAS  Google Scholar 

  • Kumar R, Kaur J, Jain S, Kumar A (2016) Optimization of laccase production from Aspergillus flavus by design of experiment technique: partial purification and characterization. J Genet Eng Biotechnol 14:125–131

    Article  Google Scholar 

  • Liu N, Shi S, Gao Y, Qin M (2009) Fiber modification of kraft pulp with laccase in presence of methyl syringate. Enzyme Microb Technol 44:89–95

    Article  CAS  Google Scholar 

  • Machado KMG, Matheus DR (2006) Biodegradation of remazol brilliant blue R by ligninolytic enzymatic complex produced by Pleurotus ostreatus. Braz J Microbiol 37:468–473

    Article  CAS  Google Scholar 

  • Nakasone KK, Lindner DL (2012) Taxonomy of Pseudolagarobasidium (Polyporales, Basidiomycota). Fungal Divers 55:155–169

    Article  Google Scholar 

  • Othman AM, Elsayed MA, Elshafei AM, Hassan MM (2018) Application of central composite design as a strategy to maximize the productivity of Agaricus bisporus CU13 laccase and its application in dye decolorization. Biocatal Agric Biotechnol 14:72–79

    Article  Google Scholar 

  • Pelaez F, Martinez MJ, Martinez AT (1995) Screening of 68 species of basidiomycetes for enzymes involved in lignin degradation. Mycol Res 99:37–42

    Article  Google Scholar 

  • Plackett RL, Burman JP (1946) The design of optimum multifactorial experiments. Biometrika 33:305–325

    Article  Google Scholar 

  • Rasak MNA, Ibrahim MF, Yee PL, Hassan MA, Abd-Aziz S (2012) Utilization of oil palm decanter cake for cellulose and polyoses production. Biotechnol Bioprocess Eng 17:547–555

    Article  Google Scholar 

  • Ries LN, Beattie SR, Espeso EA, Cramer RA, Goldman GH (2016) Diverse regulation of the CreA carbon catabolite repressor in Aspergillus nidulans. Genetics 203:335–352

    Article  CAS  Google Scholar 

  • Rongwang C, Polprasert S, Kanchanasuta S (2017) Effect of partial ozonation and thermal pretreatment on biogas production from palm oil decanter cake. Chem Eng Trans 57:1987–1992

    Google Scholar 

  • Schneider WDH, Fontana RC, Mendonca S, de Siqueira FG, Dillon AJP, Camassola M (2018) High level production of laccases and peroxidases from the newly isolated white-rot basidiomycete Marasmiellus palmivorus VE111 in a stirred-tank bioreactor in response to different carbon and nitrogen sources. Process Biochem 69:1–11

    Article  CAS  Google Scholar 

  • Senthivelan T, Kanagaraj J, Panda RC, Narayani T (2019) Screening and production of a potential extracellular fungal laccase from Penicillium chrysogenum: media optimization by response surface methodology (RSM) and central composite rotatable design (CCRD). Biotechnol Rep 23:e00344

    Article  CAS  Google Scholar 

  • Silva MLC, Souza VB, Santos VS, Kamida HM, Vasconcellos-Neto JRT, Goes-Neto A, Koblitz MGB (2014) Production of manganese peroxidase by Trametes villosa on unexpensive substrate and its application in the removal of lignin from agricultural wastes. Adv Biosci Biotechnol 5:1067–1077

    Article  Google Scholar 

  • Stajic M, Persky L, Friesem D, Hadar Y, Wasser SP, Nevo E (2006) Effect of different carbon and nitrogen sources on laccase and peroxidases production by selected Pleurotus species. Enzyme Microb Technol 38:65–73

    Article  CAS  Google Scholar 

  • Suwannasai N, Martín MP, Phosri C, Sihanonth P, Whalley AJS, Spouge JL (2013) Fungi in Thailand: a case study of the efficacy of an ITS barcode for automatically identifying species within the Annulohypoxylon and Hypoxylon genera. PLoS One 8(2):e54529. https://doi.org/10.1371/journal.pone.0054529

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Téllez-Téllez M, Fernández FJ, Montiel-González AM, Sánchez C, Díaz-Godínez G (2008) Growth and laccase production by Pleurotus ostreatus in submerged and solid-state fermentation. Appl Microbiol Biotechnol 81:675–679

    Article  Google Scholar 

  • Thakur S, Gupte A (2015) Optimization and hyper production of laccase from novel agaricomycete Pseudolagarobasidium acaciicola AGST3 and its application in in vitro decolorization of dyes. Ann Microbiol 65:185–196

    Article  CAS  Google Scholar 

  • Tien M, Kirk TK (1998) Lignin peroxidase of Phanerochaete chrysosporium. Methods Enzymol 161:238–249

    Article  Google Scholar 

  • Vaithanomsat P, Sangnam A, Boonpratuang T, Choeyklin R, Promkiam-on P, Chuntranuluck S, Kreetachat T (2015) Wood degradation and optimized laccase production by resupinate white-rot fungi in northern Thailand. BioResources 8(4):6342–6360

    Google Scholar 

  • Venkateswarulu TC, Prabhakar KV, Kumar RB, Krupanidhi S (2017) Modeling and optimization of fermentation variables for enhanced production of lactase by isolated Bacillus subtilis strain VUVD001 using artificial neural networking and response surface methodology. 3 Biotech 7:186. https://doi.org/10.1007/s13205-017-0802-x

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, New York, pp 315–322

    Google Scholar 

  • Xu H, Gua M-Y, Gao Y-H, Bai X-H, Zhou X-W (2017) Expression and characteristics of manganese peroxidase from Ganoderma lucidum in Pichia pastoris and its application in the degradation of four dyes and phenol. BMC Biotechnol 17:19. https://doi.org/10.1186/s12896-017-0338-5

    Article  CAS  PubMed  PubMed Central  Google Scholar 

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Acknowledgements

The authors are thankful to the Biodiversity-based Economy Development Office (BEDO-Thailand) for funding the research activities (Grant no. 37/2561) and The King Mongkut’s University of Technology North Bangkok (Grant no. KMUTNB-62-KNOW-07). We would like to thank Assistant Professor Dr. Rungpetch Khaengraeng for the supporting of fungal collections.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, Srinakharinwirot University, 114 Sukhumvit 23, Watthana, Bangkok, 10110, Thailand

    Pisit Thamvithayakorn

  2. Department of Biology, Faculty of Science, Nakhon Phanom University, 124 Moo 12, Ard-Samart Subdistrict, Muang District, Nakhon Phanom, 48000, Thailand

    Cherdchai Phosri

  3. Department of Agro-Industrial, Food and Environmental Technology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok, Bangkok, 10800, Thailand

    Nipon Pisutpaisal

  4. Department of Microbiology, Faculty of Science, Srinakharinwirot University, 114 Sukhumvit 23, Watthana, Bangkok, 10110, Thailand

    Sukhumaporn Krajangsang & Nuttika Suwannasai

  5. School of Pharmacy and Biomolecular Science, Liverpool John Moore University, Liverpool, L3 3AF, UK

    Anthony J. S. Whalley

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  1. Pisit Thamvithayakorn
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  2. Cherdchai Phosri
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  3. Nipon Pisutpaisal
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  4. Sukhumaporn Krajangsang
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  5. Anthony J. S. Whalley
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  6. Nuttika Suwannasai
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Contributions

PT, CP, NP and NS designed the experiments and drafted the manuscript. PT, CP and NS collected the fungal samples. PT, CP, NS and AJSW classified and identified the fungal species. PT, SK and NS analysed the data using statistical methods. AJSW edited and modified the manuscript.

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Correspondence to Nuttika Suwannasai.

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Thamvithayakorn, P., Phosri, C., Pisutpaisal, N. et al. Utilization of oil palm decanter cake for valuable laccase and manganese peroxidase enzyme production from a novel white-rot fungus, Pseudolagarobasidium sp. PP17-33. 3 Biotech 9, 417 (2019). https://doi.org/10.1007/s13205-019-1945-8

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