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Valorization of agro-residues for production of ligninolytic enzymes from Pleurotus spp. and their deployment in dye decolorisation

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Abstract  

The textile industries discharge around 10–15% dyes into effluents, posing threat to human health and environment. White-rot fungi are promising candidates for elimination of variety of dyes, owing to their potential for degradation and mineralization of a broad spectrum of highly toxic, recalcitrant, and organic pollutants. In this study, different species of Pleurotus were evaluated for ligninolytic enzymes production using paddy straw, sugarcane bagasse and wheat straw for 7, 14, 21 and 28 days of incubation, respectively. Among substrates, paddy straw exhibited maximum activity of laccase (Lac) (45968.9 ± 347.2 U/g) and manganese peroxidase (MnP) (16312.1 ± 99.9 U/g) from P. ostreatus D-66 after 28 days, however the lignin peroxidase (LiP) (10823.2 ± 94.5 U/g) activity from P. pulmonarius D-79 following 21 days. Purified Lac, MnP and LiP from P. ostreatus D-66 and P. pulmonarius D-79 showed 68.8, 44.1 and 22.8- fold increase with yield of 57.3%, 36.7% and 30.4%, respectively. These enzymes were stable over wide range of pH (3.5 to 7.5) and temperatures (30 to 50 °C) with optimum pH 4.5–5.0 and temperature 30 °C. Among synthetic dyes, the maximum decolorisation (96.9%) was achieved in Remazol brilliant blue R revealed with Lac from P. ostreatus D-66, while in P. pulmonarius D-79 (88.8%) with malachite green and decolorised dye with optimal pH 4.0–6.0 and temperature 30 °C. The findings demonstrated that paddy straw could be an attractive substrate for Pleurotus spp. to produce ligninolytic enzymes that, after purification, can be exploited effectively for degradation of synthetic dyes from textile effluents.

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Acknowledgements

Authors are thankful to The Director, ICAR-National Bureau of Agriculturally Important Microorganisms (NBAIM) for providing necessary facilities.

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Authors and Affiliations

  1. ICAR-National Bureau of Agriculturally Important Microorganisms, Mau, Uttar Pradesh, 275103, India

    Asha Singh Rajavat, Prassan Choudhary, Kuppusamy Pandiyan, Hillol Chakdar & V. Mageshwaran

  2. Amity Institute of Microbial Technology, Amity University, Noida, Uttar Pradesh, 201313, India

    Asha Singh Rajavat, Neeraj Shrivastava & Abha Agnihotri

  3. Ginning Training Centre, ICAR-Central Institute for Research on Cotton Technology (CIRCOT), Nagpur, Maharashtra, 440023, India

    Kuppusamy Pandiyan

  4. School of Water Stress Management, ICAR-National Institute for Abiotic Stress Management (NIASM), Baramati, Maharashtra, 413115, India

    Nanjappan Karthikeyan

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  1. Asha Singh Rajavat
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ASR and PC wrote first draft of this manuscript. HC, VM, NK supported for performing experiments in their laboratory. NS, KP and NK design the complete experiment and supervise during this research.

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Rajavat, A.S., Shrivastava, N., Choudhary, P. et al. Valorization of agro-residues for production of ligninolytic enzymes from Pleurotus spp. and their deployment in dye decolorisation. Biomass Conv. Bioref. (2023). https://doi.org/10.1007/s13399-023-05004-z

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