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Laccase Production by a Novel White-Rot Fungus Pseudolagarobasidium acaciicola LA 1 Through Solid-State Fermentation of Parthenium Biomass and Its Application in Dyes Decolorization

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Abstract

Parthenium sp., a belligerent noxious herbaceous wasteland weed, became a threat to environment and biodiversity, so its management is necessary to prevent future problems. A new laccase producing strain of white-rot fungus Pseudolagarobasidium acaciicola LA 1 was isolated and evaluated for laccase production through solid-state fermentation of different lignocellulosics biomass. Parthenium biomass was selected as a suitable substrate for laccase production and optimization studies were undertaken. The maximum laccase (34,444 U/g of dry weight) was achieved with Parthenium biomass as solid substrate within 7 days at 30 °C under optimized condition. SDS-PAGE and zymography confirmed the presence of single laccase of 62.8 kDa. Laccase functioned optimally at pH 4.5 and temperature of 60 °C with ABTS (2,2′-Azino-bis-3-ethylbenzothiazoline-6-sulfonic acid). The enzyme retained 94 % of its original activity at 60 °C for 30 min and was found to be highly stable within the pH ranges 4.0–7.0 for 24 h. The presence of Cu+2 (10 mM) and ethanol (10 % v/v) enhanced the laccase activity. Crude laccase was applied for decolorization of Remazol Brilliant Blue R (RBBR) and Reactive Black 5 (RB5). The biodegradation was monitored by UV–vis spectroscopy and 90 % decolorization of RBBR (100 mg/L) was observed within 4 h of incubation. This study shows the potential of P. acaciicola for production of thermostable laccase using Parthenium biomass and its application for dye decolorization.

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Acknowledgments

Authors are thankful to National Fund for Basic, Strategic and Frontier Application Research in Agriculture, India (ICAR-NFBSFARA) for funding the research activities under biofuel project (Grant No. NFBSFARA/AE2006/2010-11).

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Authors and Affiliations

  1. Division of Microbiology, Indian Agricultural Research Institute, New Delhi, 110012, India

    Anurup Adak, Rameshwar Tiwari, Surender Singh & Lata Nain

  2. Centre for Rural Development and Technology, Indian Institute of Technology Delhi, New Delhi, 110016, India

    Anurup Adak & Satyawati Sharma

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  1. Anurup Adak
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  2. Rameshwar Tiwari
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Correspondence to Lata Nain.

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Adak, A., Tiwari, R., Singh, S. et al. Laccase Production by a Novel White-Rot Fungus Pseudolagarobasidium acaciicola LA 1 Through Solid-State Fermentation of Parthenium Biomass and Its Application in Dyes Decolorization. Waste Biomass Valor 7, 1427–1435 (2016). https://doi.org/10.1007/s12649-016-9550-0

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