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N, P, or S-doped carbon nanotubes as dual mimics of NADH oxidase and cytochrome c reductase

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Abstract

Most nanozyme research is limited to oxidase and peroxidase. Here, we reported the N, P, or S doped carbon nanotubes (CNTs) for enzyme mimics of nicotinamide adenine dinucleotide (NADH) oxidase and cytochrome c (Cyt c) reductase. Through the doping of N element, the NADH oxidase-like activity of CNTs is highly improved, and the maximum initial velocity for N doped CNT (N-CNT) is increased by 4.28 times compared to that before the modification. Through the analysis of NADH oxidation products, we found that biologically active NAD+ was produced, and the oxygen was selectively reduced to water or hydrogen peroxide, which is consistent with natural NADH oxidase. Furthermore, we found for the first time that carbon nanotubes can promote the transfer of electrons from NADH to Cyt c, thereby can mimic the properties of Cyt c reductase.

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Acknowledgements

H. W., J. X. C., Q. D., X. S., Q. L., D. L., and E. K. W. thank the support from the National Key Research and Development Program of China (No. 2019YFA0709202), Natural Science Foundation of Jilin Province (No. 20220101055JC), the International Cooperation Project of Jilin Scientific and Technological Development Program (No. 20190701059GH), and the National Natural Science Foundation of China (No. 31301177).

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Authors and Affiliations

  1. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China

    Hao Wang, Jinxing Chen, Qing Dong, Xu Sun, Qiong Liu, Dan Li & Erkang Wang

  2. College of Chemistry, Jilin University, Changchun, 130012, China

    Hao Wang, Xu Sun & Erkang Wang

  3. Department of Chemistry and Physics, State University of New York at Stony Brook, Stony Brook, NY, 11794-3400, USA

    Jin Wang

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  1. Hao Wang
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  3. Qing Dong
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  4. Xu Sun
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  8. Jin Wang
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Correspondence to Dan Li or Jin Wang.

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Wang, H., Chen, J., Dong, Q. et al. N, P, or S-doped carbon nanotubes as dual mimics of NADH oxidase and cytochrome c reductase. Nano Res. 16, 6615–6621 (2023). https://doi.org/10.1007/s12274-023-5393-8

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  • DOI: https://doi.org/10.1007/s12274-023-5393-8

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