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Cadmium sulfide as bifunctional mimics of NADH oxidase and cytochrome c reductase takes effect at physiological pH

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Abstract

Recently, a study of mimic enzyme has received more attentions. However, the investigation on the oxidoreductase activity of electron mediators in the biological respiratory chain is still rare. Herein, we found that cadmium sulfide (CdS) nanorods can catalyze the formation of superoxide anions. Due to the role of the photo-generated holes and the nicotinamide adenine dinucleotide (NADH) oxidation promoted by superoxide anion \(({\rm{O}}_2^{\,\,\, \bullet - })\), the CdS exhibits NADH oxidase-like activity and can be coupled with dehydrogenase to realize the recycling of NADH. It is worth mentioning that the bio-electron acceptor, cytochrome c (Cyt c), as a chromogenic substrate, can accept electrons transferred from \({\rm{O}}_2^{\,\,\, \bullet - }\), which demonstrates the Cyt c reductase-like activity of CdS under physiological pH conditions. For different substrates, \({\rm{O}}_2^{\,\,\, \bullet - }\) induced from CdS show oxidizing capacity for NADH and reducing capacity for Cyt c, which provides a new perspective for the in-depth study of new nanozyme.

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Acknowledgements

The work was supported by the National Key R&D Program of China (No. 2019YFA0709202), MOST China (No. 2016YFA0203200), the International Cooperation Project of Jilin Scientific and Technological Development Program (No. 20190701059GH), and the National Natural Science Foundation of China (Nos. 21721003 and 31301177).

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Authors and Affiliations

  1. College of Chemistry, Jilin University, Changchun, 130012, China

    Hao Wang, Qing Dong, Xiuna Jia & Erkang Wang

  2. State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun, 130022, China

    Hao Wang, Jinxing Chen, Qing Dong, Xiuna Jia, Dan Li & Erkang Wang

  3. Department of Chemistry and Physics, State University of New York at Stony Brook, Stony Brook, NY, 11794-3400, USA

    Jin Wang

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  1. Hao Wang
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Correspondence to Dan Li or Jin Wang.

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Wang, H., Chen, J., Dong, Q. et al. Cadmium sulfide as bifunctional mimics of NADH oxidase and cytochrome c reductase takes effect at physiological pH. Nano Res. 15, 5256–5261 (2022). https://doi.org/10.1007/s12274-022-4150-8

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