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Effects of salt stress on physio-biochemical characters and gene expressions in halophyte grass Leptochloa fusca (L.) Kunth

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Abstract

Kallar grass (Leptochloa fusca) is a perennial C4 halophytic species with high salt tolerant. The present research was made to investigate the physio-biochemical characters and transcriptional changes of L. fusca under varying salinity levels (0–600 mM NaCl). The Na+ level in shoots and roots increased significantly, whereas the K+ content was maintained high in 300 mM NaCl and then declined with increasing salinity in both tissues. The content of proline in seedlings exposed to extreme salinity level was 15.5-fold higher than control. Photosynthetic pigments, total soluble proteins, PAL activity, and total phenolic compounds in salt-stressed plants increased gradually up to 450 mM and declined at 600 mM NaCl. High salt concentration led to oxidative stress that was manifested by increased MDA level. To tackle with oxidative damages, L. fusca enhanced the activity of antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX). Moreover, under NaCl stress, the mRNA levels of SOS1, PM H+-ATPase, and NHX1 were up-regulated in both tissues, but higher in roots than in shoots. Our results demonstrate that L. fusca could use an osmotic adjustment, antioxidant defense system, and regulating the ion homeostasis as the most effective salt tolerance mechanisms for better plant growth under saline conditions.

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Abbreviations

MDA:

Malondialdehyde

PM:

Plasma membrane

FW:

Fresh weight

TBA:

Tribromoarsenazo

TCA:

Trichloroacetic acid

PVP:

Polyvinylpyrrolidone

BSA:

Bovine serum albumin

NBT:

Nitro blue tetrazolium

REST:

Relative expression software tool

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Acknowledgement

The authors are grateful to Shahid Bahonar University of Kerman, Kerman, Iran for financially supporting this research.

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Authors and Affiliations

  1. Department of Biotechnology, College of Agriculture, Shahid Bahonar University of Kerman, Kerman, Iran

    Fereshteh Mohammadi, Hamid Reza Kavousi & Mehdi Mansouri

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  1. Fereshteh Mohammadi
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  2. Hamid Reza Kavousi
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  3. Mehdi Mansouri
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Correspondence to Hamid Reza Kavousi.

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Mohammadi, F., Kavousi, H.R. & Mansouri, M. Effects of salt stress on physio-biochemical characters and gene expressions in halophyte grass Leptochloa fusca (L.) Kunth. Acta Physiol Plant 41, 143 (2019). https://doi.org/10.1007/s11738-019-2935-5

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