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Salt stress effects on growth, pigments, proteins and lipid peroxidation in Salicornia persica and S. europaea

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

The effects of NaCl stress on growth, water status, contents of protein, proline, malondialdehyde (MDA), various sugars and photosynthetic pigments were investigated in seedlings of Salicornia persica and S. europaea grown in vitro. Seeds were germinated under NaCl (0, 100, 200, 300, 400, 500 and 600 mM) on Murashige and Skoog medium for 45 d. The shoot growth of both species increased under low NaCl concentration (100 mM) and then decreased with increasing NaCl concentrations. In contrast to S. persica, root length in S. europaea reduced steadily with an increase in salinity. Proline content in S. persica was higher than in S. europaea at most NaCl concentrations. Proline, reducing saccharide, oligosaccharide and soluble saccharide contents increased under salinity in both species. In contrast, contents of proteins and polysaccharides reduced in both species under salt stress. MDA content remained close to control at moderate NaCl concentrations (100 and 200 mM) and increased at higher salinities. MDA content in S. europaea was significantly higher than S. persica at higher salinities. Salt treatments decreased K+ and P contents in seedlings of both species. Significant reduction in contents of chlorophylls and carotenoids due to NaCl stress was also observed in seedlings of both species. Some differences appeared between S. persica and S. europaea concerning proteins profile. On the basis of the data obtained, S. persica is more salt-tolerant than S. europaea.

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Abbreviations

Car:

carotenoid

Chl:

chlorophyll

MDA:

malondialdehyde

MS medium:

Murashige and Skoog medium

RWC:

relative water content

WC:

water content

References

  • Agarwal, S., Pandey, V.: Antioxidant enzyme responses to NaCl stress in Cassia angustifolia.-Biol. Plant. 48: 555–560, 2004.

    Article  CAS  Google Scholar 

  • Akhani, H.: Salicornia persica Akhani (Chenopodiaceae), a remarkable new species from central Iran.-Linzer Biol. Bietr. 35: 607–612, 2003.

    Google Scholar 

  • Ajmal Khan, M., Ungar, I.A., Showalter, A. M.: The effect of salinity on growth, water status, and ion content of leaf succulent perennial halophyte, Suaeda fruticosa (L.) Forssk.-J. arid Environ. 45: 73–84, 2000.

    Article  Google Scholar 

  • Amor, N.B., Hamed, K.B., Debez, A.: Physiological and antioxidant response of the perennial halophyte Crithmum maritimum to salinity.-Plant Sci. 168: 889–899, 2005.

    Article  Google Scholar 

  • Ayala, F., O’Leary, J.W.: Growth and physiology of Salicornia bigelovii Torr. at suboptimal salinity.-Int. J. Plant Sci. 156: 197–202, 1995.

    Article  Google Scholar 

  • Bates, L.S., Walderd, R.P., Teare, I.D.: Rapid determination of free proline for water stress studies.-Plant Soil 39: 205–208, 1973.

    Article  CAS  Google Scholar 

  • Binzel, M.L., Hess, F.D., Bressan, R.A., Hasegawa, P. M.: Mechanism of adaptation to salinity in cultured glycophyte cells.-In: Cherry, J.H. (ed.): Biochemical and Physiological Mechanisms Associated with Environmental Stress Tolerance. Pp. 139–157. Springer-Verlag, Berlin 1989.

    Google Scholar 

  • Bohnert, H.J., Nelson, D.E., Jensen, R.J.: Adaptation to environmental stress.-Plant Cell 7: 1099–1011, 1995.

    Article  PubMed  CAS  Google Scholar 

  • Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principles of protein-dye binding.-Anal. Biochem. 72: 248–254, 1976.

    Article  PubMed  CAS  Google Scholar 

  • Bradley, P.H., Morris, J.T.: Relative importance of ion exclusion, secretion and accumulation in Spartina alterniflora Loisel.-J. exp. Bot. 42: 1525–1532, 1991.

    Article  CAS  Google Scholar 

  • Dubey, R.S., Singh, A.K.: Salinity induces accumulation of soluble sugars and alters the activity of sugar metabolizing enzymes in rice plants.-Biol. Plant. 42: 233–239, 1999.

    Article  CAS  Google Scholar 

  • Dubois, M., Gille, K.A., Hamilton, J.K., Rebers, P.A., Smith, F.: Colorimetric method for determination of sugars and related substance.-Anal. Chem. 28: 350–356, 1956.

    Article  CAS  Google Scholar 

  • Elshintinawy, F., Elshourbagy, M.N.: Alleviation of changes in protein metabolism in NaCl-stressed wheat seedlings by thiamine.-Biol. Plant. 44: 541–545, 2001.

    Article  CAS  Google Scholar 

  • Hassanein, A.M.: Alterations in protein and esterase patterns of peanut in response to salinity stress.-Biol. Plant. 42: 241–248, 1999.

    Article  CAS  Google Scholar 

  • Heidari-Sharifabad, H., Mirzaie-Nadoushan, H.: Salinity-induced growth and some metabolic changes in three Salsola species.-J. arid Environ. 67: 715–720, 2006.

    Article  Google Scholar 

  • Heath, R.L., Packer, L.: Photoperoxidation in isolated chloroplasts.-Arch. Biochem. Biophys. 125: 189–198, 1968.

    Article  PubMed  CAS  Google Scholar 

  • Hernandez, J.A., Almansa, M.S.: Short-term effects of salt stress on antioxidant systems and leaf water relations of pea leaves.-Physiol. Plant 115: 251–257, 2002.

    Article  PubMed  CAS  Google Scholar 

  • Kato, M., Shimizu, S.: Chlorophyll metabolism in higher plants. VI. Involvement of peroxidase in chlorophyll degeneration.-Plant Cell Physiol. 26: 1291–1301, 1985.

    CAS  Google Scholar 

  • Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head bacteriophage T4.-Nature 227: 680–685, 1970.

    Article  PubMed  CAS  Google Scholar 

  • Lichtenthaler, H., Wellburm, A.R.: Determination of total carotenoids and chlorophyll a and b of leaf extracts in different solvents.-Biochem. Soc. Trans. 603: 591–593, 1983.

    Google Scholar 

  • McCoy, T.J.: Tissue culture evaluation of NaCl tolerance in Medicago sativa species: cellular versus whole plant response.-Plant Cell Rep. 6: 31–34, 1987.

    Article  CAS  Google Scholar 

  • Meloni, D.A., Oliva, M.A., Martinez, C.A., Cambraia, J.: Photosynthesis and activity of superoxide dismutase, peroxidase, and gluthatione reductase in cotton under salt stress.-Environ. exp. Bot. 49: 69–76, 2003.

    Article  CAS  Google Scholar 

  • Moghaieb, R.E.A., Saneoka, H., Fujita, K.: Effect of salinity on osmotic adjustment, glycinebetaine accumulation and the betaine aldehyde dehydrogenase gene expression in two halophyte plants, Salicornia europaea and Suaeda maritima.-Plant Sci. 166: 1345–1349, 2004.

    Article  CAS  Google Scholar 

  • Munoz, G.E., Marin, K., Gonzalez, C.: Polypeptide profile in Prosopsis seedlings growing in saline conditions.-Phyton 61: 17–24, 1997.

    CAS  Google Scholar 

  • Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures.-Plant Physiol. 15: 473–497, 1962.

    Article  CAS  Google Scholar 

  • Nelson, N.: A Photometric adaptation of the Somogyi method for the determination of glucose.-J. biol. Chem. 153: 374–380, 1944.

    Google Scholar 

  • Niknam, V., Bagherzadeh, M., Ebrahimzadeh, H., Sokhansanj, A.: Effect of NaCl on biomass and content of sugars, proline and proteins in seedlings and leaf explants of Nicotiana tabacum grown in vitro.-Biol. Plant. 48: 613–615, 2004.

    Article  CAS  Google Scholar 

  • Niknam, V., Razavi, N., Ebrahimzadeh, H., Sharifizadeh, B.: Effect of NaCl on biomass, protein and proline contents, and antioxidant enzymes in seedlings and calli of two Trigonella species.-Biol. Plant. 50: 591–596, 2006.

    Article  CAS  Google Scholar 

  • O’Leary, J.W., Glenn, E.P., Watson, M.C.: Agriculture production of halophytes irrigated with seawater.-Plant Soil 89: 311–321, 1985.

    Article  Google Scholar 

  • Parida, A.K., Das, A.B.: Effects of NaCl stress on nitrogen and phosphorous metabolism in a true mangrove Bruguiera parviflora grown under hydroponic culture.-J. Plant Physiol. 161: 921–928, 2004.

    Article  PubMed  CAS  Google Scholar 

  • Parida, A.K., Das, A.B., Das, P.: NaCl stress causes changes in photosynthetic pigments, proteins and other metabolic components in the leaves of a true mangrove, Bruguiera parviflora, in hydroponic cultures.-J. Plant Biol. 45: 28–36, 2002.

    Article  CAS  Google Scholar 

  • Sairam, R.K., Srivastava, G.C., Agarwal, S., Meena, R.C.: Differences in antioxidant activity in response to salinity stress in tolerant and susceptible wheat genotypes.-Biol. Plant. 49: 85–91, 2005.

    Article  CAS  Google Scholar 

  • Sanchez, F.J., Manzanares, M., Andres, E. F., Tenorio, J.L., Ayerbe, L., Andres, E.F.: Turgor maintenance, osmotic adjustment and soluble sugar and proline accumulation in 49 pea cultivars in response to water stress.-Field Crops Res. 59: 225–235, 1998.

    Article  Google Scholar 

  • Scandalios, J.G.: Oxygen stress and superoxide dismutase.-Plant Physiol. 101: 7–12, 1993.

    PubMed  CAS  Google Scholar 

  • Shalata, A., Mitova, V., Volokita, M., Guy, M., Tal, M.: Response of the cultivated tomato and its wild salt-tolerant relative Lycopersicon pennelli to salt-dependent oxidative stress.-Physiol. Plant. 112: 487–494, 2001.

    Article  PubMed  CAS  Google Scholar 

  • Wheatherley, P.E.: Studies in the water relations of cotton plants. I. The field measurement of water deficit in leaves.-New Phytol. 49: 81–87, 1950.

    Article  Google Scholar 

  • Winicov, I., Bastola, D.R.: Salt tolerance in crop plants: new approaches through tissue culture and gene regulation.-Acta Physiol. Plant. 19: 435–449, 1997.

    Article  CAS  Google Scholar 

  • Zhao, K.F., Feng, L.T.: Halophyte Resources in China.-Science Press, Beijing 2001.

    Google Scholar 

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Authors and Affiliations

  1. School of Biology, University College of Science, University of Tehran, Tehran, 14155-6455, I.R. Iran

    M. Aghaleh, V. Niknam & H. Ebrahimzadeh

  2. Department of Plant Biotechnology, National Institute for Genetic Engineering and Biotechnology, Tehran, 14155-6343, I.R. Iran

    K. Razavi

Authors
  1. M. Aghaleh
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  2. V. Niknam
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  3. H. Ebrahimzadeh
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  4. K. Razavi
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Correspondence to V. Niknam.

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Aghaleh, M., Niknam, V., Ebrahimzadeh, H. et al. Salt stress effects on growth, pigments, proteins and lipid peroxidation in Salicornia persica and S. europaea . Biol Plant 53, 243–248 (2009). https://doi.org/10.1007/s10535-009-0046-7

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  • DOI: https://doi.org/10.1007/s10535-009-0046-7

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