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Identification and functional characterization of ten AP2/ERF genes in potato

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Abstract

Ethylene-responsive element-binding factors (ERF) constitute one of the largest transcription factor families in plants. In this study, we describe the cloning and the characterization of ten cDNAs encoding ERF factors from potato. The alignment of their AP2/ERF (Apetala2/ethylene-responsive factor) domain led to the identification of six StERFs (Solanum tuberosum ERFs) and four StDREBs (dehydration responsive element binding). The phylogeny and the sequence characterization allowed the classification of these StERFs into five ERF families. Expression analysis by semi-quantitative RT-PCR of these genes revealed that most of them are induced by hormone treatment such as abscisic acid, ethephon, jasmonic acid and salicylic acid. However, salt stress induced the expression of all StDREB but only three StERF genes. These results suggest that these transcription factors are involved in salt stress response. The StDREB1 and StDREB2 genes showed strong increase in expression in response to drought stress. In an attempt to improve drought tolerance in potato, we overexpressed StDREB1 and StDREB2 in transgenic potato plants (S. tuberosum L. Group Tuberosum) cv. Belle de Fontenay (BF15) and Spunta, respectively. The level of drought tolerance of these transgenic lines was significantly greater than that of wild-type control plants as measured by relative water content H2O2 content, free proline and total soluble sugars. The results suggest that the StDREB1 and StDREB2 as AP2/ERF transcription factors may play dual roles in response to drought stress in potato.

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Acknowledgments

This work was financed by the Tunisian Ministry of High Education and Scientific Research. The authors are grateful to Dr. Anne-Lise Haenni from the Institute Jacques Monod (France) for reading and improving the manuscript and to Mofida Bouaziz-Kanoun from the “Institut Supérieur d’Administration des Affaires de Sfax” (Tunisia) for her kind help with the English language.

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    Authors and Affiliations

    1. Laboratoire des Biotechnologies Végétales Appliquées à l’Amélioration des Cultures, Ecole Nationale d’Ingénieurs de Sfax, Route Soukra Km 4, BP 1173, 3038, Sfax, Tunisia

      Donia Bouaziz & Rania Jbir

    2. Unité d’Enzymes et Bioconversion, Ecole Nationale d’Ingénieurs de Sfax, Route Soukra Km 4, BP 1173, 3038, Sfax, Tunisia

      Mariam Charfeddine, Safa Charfeddine & Radhia Gargouri-Bouzid

    3. Laboratoire de Protection et Amélioration des Plantes, Centre de Biotechnologie de Sfax (CBS), BP 1177, 3018, Sfax, Tunisia

      Mohamed Najib Saidi

    4. INP-ENSA Toulouse, Génomique et Biotechnologie des Fruits, Université de Toulouse, Avenue de l’Agrobiopole, BP 32607, 31326, Castanet-Tolosan, France

      Julien Pirrello & Mondher Bouzayen

    5. UMR990 Génomique et Biotechnologie des Fruits, INRA, Chemin de Borde Rouge, 31326, Castanet-Tolosan, France

      Julien Pirrello & Mondher Bouzayen

    Authors
    1. Donia Bouaziz
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    2. Mariam Charfeddine
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    Correspondence to Donia Bouaziz.

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    Donia Bouaziz and Mariam Charfeddine contributed equally in this work.

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    Bouaziz, D., Charfeddine, M., Jbir, R. et al. Identification and functional characterization of ten AP2/ERF genes in potato. Plant Cell Tiss Organ Cult 123, 155–172 (2015). https://doi.org/10.1007/s11240-015-0823-2

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    • DOI: https://doi.org/10.1007/s11240-015-0823-2

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