Abstract
S-Adenosyl-methionine decarboxylase (SAMDC) and dehydration responsive element-binding proteins (DREBs) can improve plant resistance to abiotic stresses. These proteins have been extensively studied, but the mechanism for transcriptional regulation of SAMDC remains unclear. In this paper, the LcSAMDC2 gene and its promoter were isolated from Leymus chinensis. Two DRE cis-elements were identified from the promoter of LcSAMDC2 and shown to bind with LcDREB2. Subcellular localization and yeast one-hybrid assay revealed that LcDREB2 is a transcription factor. An electrophoretic mobility shift assay (EMSA) showed that LcDREB2 can bind to the LcSAMDC2 promoter probe containing a DRE element. Over-expression of LcDREB2 in L. chinensis callus increased expression of LcSAMDC2. Co-expression of LcDREB2 and the promoter of LcSAMDC2 fused with GUS in tobacco activated GUS activity. These results indicate that LcSAMDC2 is the downstream gene of LcDREB2. In addition, transgenic expression of LcDREB2 and LcSAMDC2 in Arabidopsis can improve the salt stress tolerance of transgenic lines. These results indicate that LcDREB2 cooperating with LcSAMDC2 contributes to resistance to abiotic stress.
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Abbreviations
- ABA:
-
Abscisic acid
- MeJA:
-
Jasmonic acid methyl ester
- DRE:
-
Dehydration responsive element
- Spd:
-
Spermidine
- Spm:
-
Spermine
- SAMDC:
-
S-adenosylmethionine decarboxylase
- EMSA:
-
Electrophoretic mobility shift assay
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This study was funded by the National Natural Science Foundation of China (30970291) and the National Basic Research Program of China (“973”, 2007CB108905).
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11240_2012_264_MOESM1_ESM.tif
The induction, transformation and identification of L. chinensis callus. A Callus induction of L. chinensis. B Co-cultivation of EHA105 (containing LcDREB2) and callus of L. chinensis. C Selection of positive transformed callus by Hygromycin B. D Untranaformed allus after GUS staining. E The positive transformed callus comfirmed by GUS staining. F Enlarged of E. 1 (TIFF 11590 kb)
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Peng, X., Zhang, L., Zhang, L. et al. The transcriptional factor LcDREB2 cooperates with LcSAMDC2 to contribute to salt tolerance in Leymus chinensis . Plant Cell Tiss Organ Cult 113, 245–256 (2013). https://doi.org/10.1007/s11240-012-0264-0
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DOI: https://doi.org/10.1007/s11240-012-0264-0