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Study on polymerization effect of polyembryony genes by SSCP marker and family trees in Chinese goats

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Abstract

The aim of experiment was to analyze the polymerization effect of genotypes and genotype combinations of PRLR and LHβ gene loci in Xinong Saanen goat by SSCP marker and family trees. The relationships of genotype combinations and litter size were compared in Xinong Saanen goat. The results indicate that there are genetic polymorphisms at the PRLR and LHβ gene loci, and there are 4 positive genotypes (GG, CC, PP and LL) and 4 negative genotypes (HH, DD, QQ and MM) effects on litter size, respectively in Xinong Saanen goat. Compared with the other genotype combinations, the polymerization effect of GGCCPPLL markedly improved (P < 0.05). The polymerization effect value of CC genotype was higher than that of CD genotype by 14.12%, and MM genotype was higher than that of LM genotype by 3.80%, and PP genotype was higher than that of QQ genotype by 15.67%, and LL genotype was higher than that of LM genotype by 11.48%, and PQ genotype was higher than that of QQ genotype by 11.02%, and CD genotype was higher than that of DD genotype by 10.69%, and PQ genotype was higher than that of PP genotype by 6.09%. There was significantly polymerization effect in the course of reproduction from parental generation (F0) to F1 generation. However, there was significantly gene isolation effect in the course of reproduction from F1 generation to F2 generation. This result can be used to guide the goat breeding in polyembryony trait.

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Acknowledgments

This study was supported by the National 863 Program of China (No. 2006BAD04A11) and the National “Eleventh Five-Year” a major scientific and technological support projects (2006BAD04A11).

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Authors and Affiliations

  1. College of Animal Science and Technology, Northwest A&F University, No. 22 Xinong Road, 712100, Yangling, Shaanxi, People’s Republic of China

    Guang Li, Xiaopeng An, Jinxing Hou, Ling Li, Dan Han, Mingming Yang, Yana Wang, Guangqin Zhu, Jiangang Wang, Yuxuan Song & Bin Yun Cao

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  1. Guang Li
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  2. Xiaopeng An
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  3. Jinxing Hou
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  4. Ling Li
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Correspondence to Bin Yun Cao.

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Guang Li, Xiaopeng An, Jinxing Hou authors equally contributed to this paper.

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Li, G., An, X., Hou, J. et al. Study on polymerization effect of polyembryony genes by SSCP marker and family trees in Chinese goats. Mol Biol Rep 38, 739–744 (2011). https://doi.org/10.1007/s11033-010-0161-8

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  • DOI: https://doi.org/10.1007/s11033-010-0161-8

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