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Single nucleotide polymorphisms in bovine PRL gene and their associations with milk production traits in Chinese Holsteins

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Abstract

Prolactin (PRL) plays a crucial role in the initiation and maintenance of lactation in mammals. In this study, seven PCR fragments representing most important functional domains of PRL gene were screened for single nucleotide polymorphisms (SNPs) in Chinese Holsteins by single-strand conformation polymorphisms and amplicons sequencing, and their genetic effects on milk production traits were evaluated. A total of four SNPs, including two in the promoter (−1043A>G and −402A>G), one in intron 1 (+2723C>T) and one in exon 4 (+8398G>A) were identified in PRL gene. Statistical results showed significant associations between the promoter genotypes and the milk performance traits in Chinese Holsteins. Cows with genotype P1-GG showed higher milk yields (P < 0.01), while cows with genotype P1-AA showed higher fat contents (P < 0.01). Haplotype analysis of two SNPs in promoter region revealed that the Hap(AG) was significantly associated with increased milk yields and Hap(AA) was associated with increased fat contents (P < 0.01). This is the second study reporting SNPs in the 5′-regulatory region of PRL gene, which interfere with milk production traits.

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Acknowledgments

This study was supported by the National 863 Program of China (No. 2006AA10Z197), National Natural Science Foundation of China (No. 30800847), National Key Technology R&D Program (No. 2006BAD01A10-5), Natural Science Foundation for Colleges and Universities in Jiangsu Province (No. 08KJD240002), The Key Discipline Fund of Jiangsu Province (No. SYC0703), Sustaining Program for Topnotch Persons of Northwest A&F University (No. 01140101), and Natural Science Foundation of Xuzhou Normal University (No. KY2007042), and Qinglan Project of Jiangsu Province. We would like to acknowledge current and former members of our laboratory for their valuable contributions and discussions.

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Authors and Affiliations

  1. Institute of Cellular and Molecular Biology, School of Life Sciences, Key Laboratory for Biotechnology on Medicinal Plants of Jiangsu Province, Xuzhou Normal University, 221116, Xuzhou, China

    Aijun Lü, Xiucai Hu, Hong Chen, Jihong Jiang, Chunlei Zhang, Haixia Xu & Xueyuan Gao

  2. Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, China

    Hong Chen

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  1. Aijun Lü
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  2. Xiucai Hu
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  3. Hong Chen
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  5. Chunlei Zhang
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  7. Xueyuan Gao
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Correspondence to Hong Chen.

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Aijun Lü, Xiucai Hu have contributed equally to this work.

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Lü, A., Hu, X., Chen, H. et al. Single nucleotide polymorphisms in bovine PRL gene and their associations with milk production traits in Chinese Holsteins. Mol Biol Rep 37, 547–551 (2010). https://doi.org/10.1007/s11033-009-9762-5

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  • DOI: https://doi.org/10.1007/s11033-009-9762-5

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