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Two novel SNPs in coding region of the caprine Fat-inducing transcript gene and their association with growth traits

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Abstract

Fat-inducing transcript (FIT) are endoplasmic reticulum resident membrane proteins that induce lipid droplet accumulation. It plays a crucial role in the fundamental process of fat storing. In this study, on the basis of PCR–SSCP and DNA sequencing methods, polymorphisms of the FIT gene were detected in 254 individuals from three goat breeds. The results showed that only P5 locus had two SNPs, resulting in a synonymous mutation (NM_001103095: m.422T > A/G resulting in S124S) and a missense mutation (NM_001103095: m.454 A > G resulting in Q135R). The associations between polymorphic loci and the growth traits of Boer and Xuhuai goat were analysed, and significant association were found in body height and chest circumference index (P < 0.05). Genotype BB of the Boer was associated with a higher chest circumference index (P < 0.05) than Genotype AC. In Xuhuai breed, Genotype BB was associated with a lower body height (P < 0.05) than Genotype AA. However, Genotype BB and AC had greater chest circumference index than Genotype AA (P < 0.01).

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Acknowledgments

This work was supported by Natural Science Foundation of Jiangsu Province (No. BK2008120), the “13115” Sci-Tech innovation program of Shaanxi province (2008ZDKG-11), Research Fund for the Doctor Program of Higher Education of China (No. 20080712001). Talents Foundation of Northwest A&F University (No.01140411), the National “863” Program of the People’s Republic of China (No. 2008AA10Z138).

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Authors and Affiliations

  1. Institute of Cellular and Molecular Biology, College of Life Science, Xuzhou Normal University, 221116, Xuzhou, Jiangsu, People’s Republic of China

    Jiajie Sun, Chunlei Zhang, Hong Chen, Xingtang Fang, Qijiang Jin, Danxia Chen, Xiuying Shi & Yu Du

  2. Shaanxi Key Laboratory of Molecular Biology for Agriculture, College of Animal Science and Technology, Northwest A&F University, 712100, Yangling, Shaanxi, China

    Hong Chen

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  1. Jiajie Sun
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  2. Chunlei Zhang
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  3. Hong Chen
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  4. Xingtang Fang
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  5. Qijiang Jin
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  6. Danxia Chen
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  7. Xiuying Shi
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  8. Yu Du
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Correspondence to Hong Chen.

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Sun, J., Zhang, C., Chen, H. et al. Two novel SNPs in coding region of the caprine Fat-inducing transcript gene and their association with growth traits. Mol Biol Rep 37, 485–490 (2010). https://doi.org/10.1007/s11033-009-9672-6

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  • DOI: https://doi.org/10.1007/s11033-009-9672-6

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