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Marker-assisted pyramiding of two cereal cyst nematode resistance genes from Aegilops variabilis in wheat

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Abstract

The cereal cyst nematode (CCN) Heterodera avenae, is a significant pathogen of wheat. The wild grass Aegilops variabilis Accession No.1 has been found to be resistant to pathotypes of CCN; at least two genes transferred to wheat, designated as CreX and CreY, are involved in the resistance response. The CreY gene may be the same as Rkn-mn1, which confers resistance to root knot nematode (RKN) Meloidogyne naasi. The objective of this work was to pyramid the two CCN resistance genes in a wheat background through marker-assisted selection. As a first step, molecular markers flanking CreX were identified. The completely linked RAPD marker of Rkn-mn1 (CreY), OpY16-1065, previously obtained, was converted into a SCAR. All these dominant markers were used to incorporate in the same genotype the two Ae. variabilis chromosome segments carrying the two genes for resistance. CCN bioassays with the Ha12 pathotype showed that the level of resistance of the pyramided line was significantly higher than that of CreX and CreY single introgression lines, but lower than that of Ae. variabilis. This study thus illustrates the utilization of molecular markers in breeding for host resistance.

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Acknowledgements

The authors would like to thank E. Jenczewski for his critical reading of the manuscript, and M. Trottet for advice on statistical analyses. We are grateful to Victoria Hawken for correcting English.

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  1. UMR INRA-Agrocampus Rennes, Amélioration des Plantes et Biotechnologies Végétales, Domaine de la Motte, BP, 35 327, 35 653, Le Rheu Cedex, France

    Dominique Barloy, Jocelyne Lemoine, Paulette Abelard, A. M. Tanguy & Joseph Jahier

  2. UMR INRA-Agrocampus Rennes, Biologie des Organismes et des Populations appliqué à la protection des Plantes (BiO3P) Domaine de la Motte, BP, 35 327, 35 653, Le Rheu Cedex, France

    Roger Rivoal

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  1. Dominique Barloy
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  2. Jocelyne Lemoine
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  3. Paulette Abelard
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  4. A. M. Tanguy
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  5. Roger Rivoal
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  6. Joseph Jahier
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Correspondence to Dominique Barloy.

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Barloy, D., Lemoine, J., Abelard, P. et al. Marker-assisted pyramiding of two cereal cyst nematode resistance genes from Aegilops variabilis in wheat. Mol Breeding 20, 31–40 (2007). https://doi.org/10.1007/s11032-006-9070-x

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  • DOI: https://doi.org/10.1007/s11032-006-9070-x

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