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Association between variants in the 5′-untranslated region of the bovine MC4R gene and two growth traits in Nanyang cattle

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Abstract

Melanocortin-4 receptor (MC4R) is one of five G-protein-coupled receptors binding melanocortins that is implicated in the control of feeding behavior and energy homeostasis. Six cattle populations (n = 594), including four Chinese indigenous breeds, Chinese Holstein, and a meat type breed (Angus), were used to detect single nucleotide polymorphisms in 5′-untranslated region of MC4R gene by means of PCR–SSCP and DNA sequencing. Four linked SNPs (g.[−293C>G; −193A>T; −192T>G; −129A>G]) were identified. The g.−293C>G and g.−129A>G could be genotyped with a PCR–RFLP using TaiI in three combined genotypes (AA, AB and BB). The two linked SNPs were associated with body weight and daily gain in Nanyang aged 6 months (P < 0.05), but they had no significant effect on body weight and daily gain in Nanyang aged 24 months (P > 0.05).

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Acknowledgments

This study was supported by the National 863 Program of China (No. 2006AA10Z197), National Natural Science Foundation of China (No. 30771544), National Key Technology R&D Program (No. 2006BAD01A10-5), Innovative Foundation of Outstanding Talent from Henan Province (No. 0521001900), Sustaining Program for Topnotch Persons of Northwest A&F University (No. 01140101), and Natural Science Foundation of Xuzhou Normal University (No. 2003XY234; No. 07XLA08; No. KY2007019).

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Authors and Affiliations

  1. College of Animal Science and Technology, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Northwest A & F University, Yangling, Shaanxi, People’s Republic of China

    Chun-Lei Zhang, Hong Chen, Xian-Yong Lan & Chu-Zhao Lei

  2. Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, Jiangsu, People’s Republic of China

    Chun-Lei Zhang, Yan-Hong Wang, Hong Chen & Xing-Tang Fang

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  1. Chun-Lei Zhang
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  2. Yan-Hong Wang
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  3. Hong Chen
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Correspondence to Hong Chen.

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Zhang, CL., Wang, YH., Chen, H. et al. Association between variants in the 5′-untranslated region of the bovine MC4R gene and two growth traits in Nanyang cattle. Mol Biol Rep 36, 1839–1843 (2009). https://doi.org/10.1007/s11033-008-9388-z

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  • DOI: https://doi.org/10.1007/s11033-008-9388-z

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