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Molecular cytogenetic identification of a wheat–Aegilops geniculata Roth 7Mg disomic addition line with powdery mildew resistance

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Abstract

Aegilops geniculata Roth is an important germplasm resource for the transfer of beneficial genes into common wheat (Triticum aestivum L.). A new disomic addition line NA0973-5-4-1-2-9-1 was developed from the BC1F6 progeny of the cross wheat cv. Chinese Spring (CS)/Ae. geniculata SY159//CS. We characterized this new line by morphological and cytogenetic identification, analysis of functional molecular markers, genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), and disease resistance evaluation. Cytological observations suggested that NA0973-5-4-1-2-9-1 contained 44 chromosomes and formed 22 bivalents at meiotic metaphase I. The GISH investigations showed that the line contained 42 wheat chromosomes and a pair of Ae. geniculata chromosomes. EST-STS multiple loci markers and PLUG (PCR-based landmark unique gene) markers confirmed that the introduced Ae. geniculata chromosomes belonged to homoeologous group 7. FISH identification suggested that NA0973-5-4-1-2-9-1 possessed an additional pair of 7Mg chromosomes, and at the same time, there were structural differences in a pair of 6D chromosomes between NA0973-5-4-1-2-9-1 and TA7661 (CS-AEGEN DA 7Mg). After inoculation with powdery mildew (Blumeria graminis f. sp. tritici, Bgt) isolates E09, NA0973-5-4-1-2-9-1 exhibited a powdery mildew resistance infection type different from that of TA7661, and we conclude that the powdery mildew resistance of NA0973-5-4-1-2-9-1 originated from its parent Ae. geniculata SY159. Therefore, NA0973-5-4-1-2-9-1 can be used as a donor source for introducing novel disease resistance genes into wheat during breeding programs with the assistance of molecular and cytogenetic markers.

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Acknowledgments

The authors would like to thank Dr. Shulan Fu and Prof. Zongxiang Tang (Sichuan Agricultural University, China) for technical guidance in GISH and FISH analysis. This work was supported by the National Natural Science Foundation of China (No. 31471481), the Shaanxi Natural Science Foundation (No. 2015JQ3078), and the Basic Scientific Funding Project of the Northwest A&F University (2014YB080, 2452015268, 2013BSJJ056).

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    1. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China

      Yajuan Wang, Wei Quan, Nana Peng, Changyou Wang, Xiaofei Yang, Xinlun Liu, Hong Zhang, Chunhuan Chen & Wanquan Ji

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    1. Yajuan Wang
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    Correspondence to Wanquan Ji.

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    Yajuan Wang and Wei Quan have contributed equally to this article.

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    Wang, Y., Quan, W., Peng, N. et al. Molecular cytogenetic identification of a wheat–Aegilops geniculata Roth 7Mg disomic addition line with powdery mildew resistance. Mol Breeding 36, 40 (2016). https://doi.org/10.1007/s11032-016-0463-1

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