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DNA fingerprinting and genetic diversity analysis in Asparagus officinalis L. cultivars using microsatellite molecular markers

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Abstract

Garden asparagus (Asparagus officinalis L.) is known as a valuable genetic resource for both vegetables and medicinal purposes. However, little is known about the organization and diversity of repetitive DNA sequences, and the usefulness of such resources in analyzing genetic diversity and DNA fingerprinting in A. officinalis cultivars. In this study, a large-scale genome-wide identification of microsatellite molecular markers in A. officinalis genome was performed. Genetic diversity and DNA fingerprinting of 24 cultivars were carried out using the information obtained from polymorphic simple sequence repeats (SSRs) markers. The polymorphism information content were found in the range of 0.21–0.43 with an average of 0.35. The genetic distance of 24 cultivars were ranged from 0.26 to 1.07, with an average value of 0.75, indicating relatively high genetic diversity among these accessions. The dendrogram constructed by unweighted pair group method with arithmetic mean clustering method classified these cultivars into two discrete groups revealing the genetic relatedness and diversity among these cultivars. Furthermore, the 24 cultivars were fingerprinted using three SSR primers. Our results showed that the primer pair Asp-SSR-2-C6 enabled the identification of 12 cultivars, whereas Asp-SSR-3-C10 and Asp-SSR-6-C4 primers could differentiate 13 and 10 cultivars, respectively. The present study deciphered the reliability of SSR markers for DNA fingerprinting and genetic diversity analysis in asparagus cultivars, which will facilitate the conservation, breeding and future genetic studies.

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Acknowledgements

The authors would like to thank the Shandong Ju Xin Yuan Agriculture Technology Co. Ltd and Shandong Yuncheng Jiuyuan Agriculture Technology Co. Ltd, Heze, China for providing plant materials for this study.

Funding

This work was supported by Shandong Agricultural Science and Technology Program (industrial upgrading of agricultural park) (2019YQ004), National Natural Science Foundation of China (31861143009), Taishan Scholar Project of Shandong Province (ts20190964), High-level Foreign Experts Introduction Program (GL20200123001), Agricultural scientific and technological innovation project of Shandong Academy of Agricultural Sciences (CXGC2018E13).

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Authors and Affiliations

  1. Shandong Provincial Key Laboratory of Crop Genetic Improvement, Ecology and Physiology, Institute of Crop Germplasm Resources (Institute of Biotechnology), Shandong Academy of Agricultural Sciences, Jinan, 250100, People’s Republic of China

    Naveed Ahmad, Ruizheng Tian, Jindong Lu, Guanghui Li, Jie Sun, Ruxia Lin, Chuanzhi Zhao, Shuzhen Zhao & Xingjun Wang

  2. College of Life Sciences, Shandong Normal University, Jinan, 250014, People’s Republic of China

    Jie Sun, Ruxia Lin, Chuanzhi Zhao, Shuzhen Zhao & Xingjun Wang

  3. Shandong Ju Xin Yuan Agriculture Technology Co. Ltd, Heze, 274400, People’s Republic of China

    Changsheng Zhou

  4. Shandong Yuncheng Jiuyuan Agriculture Technology Co. Ltd, Heze, 274700, People’s Republic of China

    Huaxing Chang

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  1. Naveed Ahmad
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  2. Ruizheng Tian
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  3. Jindong Lu
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  4. Guanghui Li
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  7. Chuanzhi Zhao
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  8. Changsheng Zhou
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  9. Huaxing Chang
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  10. Shuzhen Zhao
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  11. Xingjun Wang
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Contributions

All authors contributed to the study conception and design. Material preparation, data collection and analysis were performed by N Ahmad, R Tian and J Lu. The study was conceptualized by S Zhao and X Wang. Formal analysis was conducted by G Li, J Sun, R Lin, and C Zhao. Plant materials were provided by C Zhou and H Chang. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Shuzhen Zhao or Xingjun Wang.

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Ahmad, N., Tian, R., Lu, J. et al. DNA fingerprinting and genetic diversity analysis in Asparagus officinalis L. cultivars using microsatellite molecular markers. Genet Resour Crop Evol 70, 1163–1177 (2023). https://doi.org/10.1007/s10722-022-01493-5

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