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Identification and characterization of microsatellite markers for the population genetic structure in endemic red-tailed barb, Gonoproktopterus curmuca

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Abstract

Gonoproktopterus curmuca is an endangered red tailed barb found in Southern part of Western Ghat, India. As a part of stock-specific, propagation assisted rehabilitation and management program, polymorphic microsatellites markers were used to study the genetic diversity and population structure of this species from the three River systems of Southern Western Ghats, such as Periyar River, the Chalakkudy River, and the Chaliyar River. From selected eight polymorphic microsatellite markers, the number of alleles per locus ranged from 2 to 8, and the average number of alleles among 3 populations ranged from 5.0 to 5.75. The mean observed (Hob) and expected (Hex) heterozygosity ranged from 0.5148 to 0.5360 and from 0.5996 to 0.6067, respectively. Significant deviations from Hardy–Weinberg Equilibrium expectation were found at majority of the loci (except Gcur MFW72 and Gcur MFW19) and in all three populations in which heterozygote deficits were apparent. The analysis of molecular variance indicates that the percent of variance among populations and within populations were 6.73 and 93.27, respectively. The pairwise FST values between populations indicate that there were significant deviations in genetic differentiations for the red-tailed barb populations from these three Rivers of the Western Ghats, India. The microsatellites methods reported a low degree of gene diversity and lack of genetic heterogeneity in the population of G. curmuca, which strongly emphasize the need of fishery management, conservation and rehabilitation of G. curmuca.

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Abbreviations

AMOVA:

Analysis of molecular variance

CAMP:

Conservation assessment management plan

CHD:

Chalakkudy River

CLR:

Chaliyar River

DNA:

Deoxyribo nucleic acid

FIS :

Coefficient of inbreeding or heterozygosity deficient

FST :

Coefficient of genetic differentiation

Hex :

Expected heterozygosity

Hob :

Observed heterozygosity

HWE:

Hardy–Weinberg equilibrium

IUCN:

International Union for Conservation of Nature and Natural Resources

Na:

Number of observed alleles

NBFGR:

National Bureau of Fish Genetic Resources

NCBI:

National Centre for Biotechnology Information

Ne:

Number of expected alleles

OD:

Optical density

PCR:

Polymerase chain reaction

PER:

Periyar River

UPGMA:

Unweighted pair-group method with arithmetic mean

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Acknowledgments

This study was financially supported by NATP-ICAR (MM-III-18) to AG. The authors are thankful to Dr. WS Lakra, Dr. MJ Modayil and Dr. SP Singh for encouragement, support and guidance.

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Author notes

  1. A. G. Ponniah

    Present address: Central Institute of Brackishwater Aquaculture, RA Puram, Chennai, 600028, Tamilnadu, India

Authors and Affiliations

  1. National Bureau of Fish Genetic Resources (NBFGR) Cochin Unit, CMFRI Campus, Cochin, 682 018, Kerala, India

    K. K. Musammilu, P. M. Abdul-Muneer, A. Gopalakrishnan & V. S. Basheer

  2. NBFGR, Canal Ring Road, P.O. Dilkusha, Lucknow, 226002, UP, India

    Harishankar Gupta, Vindhya Mohindra, Kuldeep K. Lal & A. G. Ponniah

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  1. K. K. Musammilu
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  2. P. M. Abdul-Muneer
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Correspondence to P. M. Abdul-Muneer.

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Musammilu, K.K., Abdul-Muneer, P.M., Gopalakrishnan, A. et al. Identification and characterization of microsatellite markers for the population genetic structure in endemic red-tailed barb, Gonoproktopterus curmuca . Mol Biol Rep 41, 3051–3062 (2014). https://doi.org/10.1007/s11033-014-3164-z

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