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Genetic Diversity of Asian Cotton (Gossypium arboreum L.) in China Evaluated by Microsatellite Analysis

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Abstract

Asian cotton (Gossypium arboreum L.) was once widely cultivated in China. It has also been a valuable source of genetic variation in modern cotton improvement. In this study, the genetic diversity of selected G. arboreum accessions collected from different regions of China was evaluated by microsatellite (simple sequence repeats, SSRs) analysis. Of the 358 microsatellite markers analyzed, 74 primer pairs detected 165 polymorphic DNA fragments among 39 G. arboreum accessions examined. Twelve accessions could be fingerprinted with one or more SSR markers. With the exception of two accessions, DaZiJie and DaZiMian, genetic similarity coefficients among all accessions ranged from 0.58 to 0.87 suggesting high level of genetic variation in the G. arboreum collections. The UPGMA dendrogram constructed from genetic similarity coefficients revealed positive correlation between cluster groupings and geographic distances. In addition, comparison of the microsatellite amplification profiles of the diploid G. arboreum and tetraploid Gossypium hirsutum L. found that size distribution of amplified products in G. arboreum was dispersive and that of G. hirsutum was relatively concentrated. The information on the genetic diversity and SSR fingerprinting from this study is useful for developing mapping populations for constructing diploid cotton genetic linkage map and tagging economically important traits.

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References

  • A.M. Abdalla, O.U.K. Reddy, K.M. El-Zik and A.E. Pepper, Genetic diversity and relationships of diploid and tetraploid cottons revealed using AFLP. Theor. Appl. Genet. 102 (2001) 222-229

    Article  CAS  Google Scholar 

  • J.O. Beasley, The origin of American tetraploid Gossypium species. Am. Nat. 74 (1940) 285-286

    Article  Google Scholar 

  • J.O. Beasley, Meiotic chromosome behavior in species, species hybrids, haploids and induced polyploids of Gossypium. Genetics 27 (1942) 25-54

    CAS  PubMed  Google Scholar 

  • D.U. Gerstel, Chromosomal translocations in interspecific hybrids of the genus Gossypium. Evolution 7 (1953) 234-244

    Article  Google Scholar 

  • O.A. Gutiérrez, S. Basu, S. Saha, J.N. Jenkins, D.B. Shoemaker, C.L. Cheatham and J.C. McCarty Jr., Genetic distance among selected cotton genotypes and its relationship with F2 performance. Crop Sci. 42 (2002) 1841-1847

    Google Scholar 

  • M.J. Iqbal, O.U.K. Reddy, K.M. El-Zik and A.E. Pepper, A genetic bottleneck in the 'evolution under domesticationȁ9 of upland cotton Gossypium hirsutum L. examined using DNA fingerprinting. Theor. Appl. Genet. 103 (2001) 547-554

    Article  CAS  Google Scholar 

  • C.X. Jiang, R.J. Wright, K.M. El-Zik and A.H. Paterson, Polyploid formation created unique avenues for response to selection in Gossypium (cotton). Proc. Natl. Acad. Sci. USA 95 (1998) 4419-4424

    Article  CAS  Google Scholar 

  • S.A. Khan, D. Hussain, E. Askari, J.McD. Stewart, K.A. Malik and Y. Zafar, Molecular phylogeny of Gossypium species by DNA fingerprinting. Theor. Appl. Genet. 101 (2000) 931-938

    Article  CAS  Google Scholar 

  • J.M. Lacape, T.B. Nguyen, S. Thibivilliers, B. Bojinov, B. Courtois, R.G. Cantrell, B. Burr and B. Hau, A combined RFLP-SSR-AFLP map of tetraploid cotton based on a Gossypium hirsutum × Gossypium barbadense backcross population. Genome 46 (2003) 612-626

    Article  PubMed  CAS  Google Scholar 

  • S. Liu, S. Saha, D. Stelly, B. Burr and R.G. Cantrell, Chromosomal assignment of microsatellite loci in cotton. J. Hered. 91 (2000) 326-332

    Article  PubMed  CAS  Google Scholar 

  • H.J. Lu and G.O. Myers, Genetic relationships and discrimination of ten influential Upland cotton varieties using RAPD markers. Theor. Appl. Genet. 105 (2002) 325-331

    Article  PubMed  CAS  Google Scholar 

  • S.S. Mehetre, A.R. Aher, V.L. Gawande, V.R. Patil and A.S. Mokate, Induced polyploidy in Gossypium: a tool to overcome interspecific incompatibility of cultivated tetraploid and diploid cottons. Curr. Sci. 84 (2003) 1510-1512

    Google Scholar 

  • M. Mei, N.H. Syed, W. Gao, P.M. Thaxton, C.W. Smith, D.M. Stelly and Z.J. Chen, Genetic mapping and QTL analysis of fiber–related traits in cotton (Gossypium). Theor. Appl. Genet. 108 (2004) 280-291

    Article  CAS  Google Scholar 

  • J.V. Monte, C.L. McIntyre and J.P. Gustafson, Analysis of phylogenetic relationships in the Triticeae tribe using RFLPs. Theor. Appl. Genet. 86 (1993) 649-655

    Article  Google Scholar 

  • M. Morgante, M. Hanafey and W. Powell, Microsatellites are preferentially associated with nonrepetitive DNA in plant genomes. Nat. Genet. 30 (2002) 194-200

    Article  PubMed  CAS  Google Scholar 

  • M. Nei and W.H. Li, Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Natl. Acad. Sci. USA 76 (1979) 5269-5273

    Article  PubMed  CAS  Google Scholar 

  • A.H. Paterson, C.L. Brubaker and J.F. Wendel, A rapid method for extraction of cotton (Gossypium spp.) genome DNA suitable for RFLP or PCR analysis. Plant Mol. Biol. Rep. 11 (1993) 122-127

    CAS  Google Scholar 

  • F.J. Rohlf, NTSYS-pc: Numerical Taxonomy and Multivariate Analysis SystemVersion 2.1, User Guide. New York: Exeter Software (2000).

    Google Scholar 

  • T. Seelanan, A. Schnabel and J.F. Wendel, Congruence and consensus in the cotton tribe. Syst. Bot. 22 (1997) 259-290

    Article  Google Scholar 

  • A. Sharma, R. Sharma and H. Machii, Assessment of genetic diversity in a Morus germplasm collection using fluorescence-based AFLP markers. Theor. Appl. Genet. 101 (2000) 1049-1055

    Article  CAS  Google Scholar 

  • R.R. Sokal and C.D. Michener, A statistical method for evaluating systematic relationships. Univ. Kansas Sci. Bull. 28 (1958) 1409-1438

    Google Scholar 

  • N.H. Syed, H.S. Lee, M. Mei, P. Thaxton, D.M. Stelly and Z.J. Chen, Variability and evolution of microsatellite loci in cotton (Gossypium) diploid and polyploidy genomes. San DiegoCA: Plant & Animal Genome IX Conference. Town & Country Hotel (2001).

    Google Scholar 

  • V. Tatineni, R.G. Cantrell and D.D. Davis, Genetic diversity in elite cotton germplasm determined by morphological characteristics and RAPDs. Crop Sci. 36 (1996) 186-192

    Article  Google Scholar 

  • G.V. Vergara and S.S. Bughrara, AFLP analyses of genetic diversity in Bentgrass. Crop Sci. 43 (2003) 2162-2171

    Article  CAS  Google Scholar 

  • X.L. Xiang and D.Z. Shen, Chinese Asian Cotton (Gossypium arboreum). Beijing, China: China Agricultural Press (1989).

    Google Scholar 

  • Q.H. Xu, X.L. Zhang and Y.C. Nie, Genetic diversity evaluation of cultivars (G. hirsutum L.) from the Changjiang River valley and Yellow River valley by RAPD markers. Acta Genet. Sin. 28 (2001) 683-690

    PubMed  CAS  Google Scholar 

  • L.F. Zhu, X.L. Zhang and Y.C. Nie, Analysis of genetic diversity in upland cotton (Gossypium hirsutum L.) cultivars from China and foreign countries by RAPDs and SSRs. J. Agric. Biotechnol. 11 (2003) 450-455

    CAS  Google Scholar 

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Authors and Affiliations

  1. National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, Hubei, 430070, China

    Diqiu Liu, Xiaoping Guo, Zhongxu Lin, Yichun Nie & Xianlong Zhang

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  1. Diqiu Liu
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  2. Xiaoping Guo
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  3. Zhongxu Lin
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  4. Yichun Nie
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  5. Xianlong Zhang
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Correspondence to Xianlong Zhang.

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Diqiu Liu, Xiaoping Guo: These two authors contributed equally to this work.

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Liu, D., Guo, X., Lin, Z. et al. Genetic Diversity of Asian Cotton (Gossypium arboreum L.) in China Evaluated by Microsatellite Analysis. Genet Resour Crop Evol 53, 1145–1152 (2006). https://doi.org/10.1007/s10722-005-1304-y

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  • DOI: https://doi.org/10.1007/s10722-005-1304-y

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