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SSR-Based DNA Fingerprinting and Diversity Assessment Among Indian Germplasm of Euryale ferox: an Aquatic Underutilized and Neglected Food Crop

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Abstract

Euryale ferox is native to Southeast Asia and China, and it is one of the important aquatic food crops propagated mostly in eastern part of India. The aim of the present study was to characterize and evaluate the genetic diversity of ex situ collections of E. ferox germplasm from different geographical states of India using microsatellite (simple sequence repeats (SSRs)) markers. Ten SSR markers were analyzed to assess DNA fingerprinting and genetic diversity of 16 cultivated germplasm of E. ferox. Total 37 polymorphic alleles were recorded with an average of 3.7 allele frequency per primer. The polymorphic information content value varied from 0.204 to 0.735 with mean of 0.448. A high range of heterozygosity (Ho 0.228; He 0.512) was detected in the present study. The neighbor-joining (N-J) tree and the principle coordinate analysis showed that the germplasm divided in to three main clusters. The results of the present investigation comply that SSR markers are effective for computing genetic assessment of genetic diversity and similarity with classifying cultivated varieties of E. ferox. Evaluation of genetic diversity among Indian E. ferox germplasm could provide useful information for genetic improvement.

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Acknowledgments

The authors thankfully acknowledge the ICAR-RCER-Research Centre Makhana, Darbhanga, for providing the germplasm of E. ferox.

Funding

Financial assistance (Grant No. SB/YS/LS-136/2013) from DST-SERB, New Delhi, India, is also acknowledged.

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Authors and Affiliations

  1. Centre for Biological Sciences (Biotechnology), School of Earth, Biological and Environmental Sciences, Central University of South Bihar, Patna, Bihar, 800014, India

    Nitish Kumar & Divya Shikha

  2. Centre for Education (Life Science), Central University of South Bihar, Gaya Campus, Gaya, Bihar, 823001, India

    Swati Kumari

  3. ICAR-National Institute of Biotic Stress Management, Baronda Farm, Raipur, Chhattisgarh, 493225, India

    Binod Kumar Choudhary

  4. ICAR-Indian Institute of Wheat & Barley Research, Karnal, Haryana, 132001, India

    Lokendra Kumar

  5. ICAR RCER-Research Centre for Makhana, Darbhanga, Bihar, 846005, India

    Indu Shekhar Singh

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  1. Nitish Kumar
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  2. Divya Shikha
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  3. Swati Kumari
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  4. Binod Kumar Choudhary
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Correspondence to Nitish Kumar.

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Kumar, N., Shikha, D., Kumari, S. et al. SSR-Based DNA Fingerprinting and Diversity Assessment Among Indian Germplasm of Euryale ferox: an Aquatic Underutilized and Neglected Food Crop. Appl Biochem Biotechnol 185, 34–41 (2018). https://doi.org/10.1007/s12010-017-2643-9

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  • DOI: https://doi.org/10.1007/s12010-017-2643-9

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