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Identification and evaluation of disease resistance and HMW-GS composition of Aegilops geniculata Roth

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Abstract

Aegilops geniculata Roth is an important germplasm resource for the transfer of beneficial genes from alien species of wheat into common wheat (Triticum aestivum L.). In this study, we identified and evaluated agronomic characters, powdery mildew resistance, stripe rust resistance, and high molecular weight glutenin subunit (HMW-GS) compositions of 44 Ae. geniculata accessions. The average growth period (254 days) was longer than that of common wheat (240 days). Coefficients of variation (CVs) of all examined agronomic characters except growth period were >10 %. The largest CV, 23.80 %, was that of 1,000-grain weight. An assay for disease resistance revealed that 37 Ae. geniculata accessions (85.09 %) were resistant to powdery mildew, of which 33 were immune, 1 was highly resistant, and 3 were moderately resistant. This assay also indicated that 33 of the accessions (75 %) were resistant to stripe rust, with 25 showing immunity, 4 highly resistant, and 4 moderately resistant. Additionally, 19 accessions (43.18 %) were immune to both powdery mildew and stripe rust. HMW-GSs in the 44 accessions were analyzed by SDS polyacrylamide-gel electrophoresis, which indicated rich germplasm diversity. A total of 21 different HMW-GS alleles were identified at Glu-1 loci. The most frequent subunits were 1Dx2, 1Dx1.1, 1Dx2.2, 1Dx1.5, 1Bx20, 1Bx22, 1Dy10, and 1Dy12, found in 36.36, 54.55, 40.91, 34.09, 38.64, 25.00, 47.73, and 20.45 % of accessions, respectively. The data uncovered for the 44 accessions should facilitate their use as beneficial germplasm donors in wheat breeding.

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References

  • Bariana HS, McIntosh R (1993) Cytogenetic studies in wheat. XV. Location of rust resistance genes in VPM1 and their genetic linkage with other disease resistance genes in chromosome 2A. Genome 36:476–482

    Article  CAS  PubMed  Google Scholar 

  • Bochev B, Christova S, Doncheva V (1982) The genus Aegilops possibilities and perspectives of utilization in the breeding of high quality wheat cultivars. In: Proceedings VII world cereal and bread congress, Prague

  • Chen XM (2005) Epidemiology and control of stripe rust [Puccinia striiformis f. sp. tritici] on wheat. Can J Plant Pathol 27:314–337

    Article  Google Scholar 

  • Chen XM, Moore M, Milus EA, Long DL, Line RF, Marshall D, Jackson L (2002) Wheat stripe rust epidemics and races of Puccinia striiformis f. sp tritici in the United States in 2000. Plant Dis 86:39–46

    Article  Google Scholar 

  • Chen WQ, Wu LR, Liu TG, Xu SC, Jin SL, Peng YL, Wang BT (2009) Race dynamics, diversity, and virulence evolution in Puccinia striiformis f. sp. tritici, the causal agent of wheat stripe rust in China from 2003 to 2007. Plant Dis 93:1093–1101

    Article  Google Scholar 

  • Ciaffi M, Dominici L, Lafiandra D, Porceddu E (1992) Seed storage-proteins of wild wheat progenitors and their relationships with technological properties. Hereditas 116:315–322

    Article  Google Scholar 

  • Cox TS, Gill BS (1992) Use of diploid progenitors to improve leaf rust resistance in hexaploid wheat. Vortr Planzenzücht 24:185–187

    Google Scholar 

  • Friebe BR, Jiang J, Raupp WJ, McIntosh RA, Gill BS (1996) Characterization of wheat-alien translocations conferring resistance to diseases and pests: current status. Euphytica 91:59–87

    Article  Google Scholar 

  • Friebe BR, Tuleen NA, Gill BS (1999) Development and identification of a complete set of Triticum aestivum—Aegilops geniculata chromosome addition lines. Genome 42:374–380

    Article  Google Scholar 

  • Gill BS, Sharma HC, Raupp WJ, Browder LE, Hatchett JH, Harvey TL, Moseman JG, Waines JW (1985) Evaluation of Aegilops species for resistance to powdery mildew, wheat leaf rust, Hessian fly, and greenbug. Plant Dis 69:314–316

    Google Scholar 

  • Guo XM, Guo JX, Li XQ, Yang XM, Li LH (2010) Molecular characterization of two novel Glu-D1-encoded subunits from Chinese wheat (Triticum aestivum L.) landrace and functional properties of flours possessing the two novel subunits. Genet Resour Crop Evol 57:1217–1225

    Article  CAS  Google Scholar 

  • Hsam SLK, Lapochkina IF, Zeller FJ (2003) Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.). 8. Gene Pm32 in a wheat-Aegilops speltoides translocation line. Euphytica 133:367–370

    Article  Google Scholar 

  • Hu XG, Wu BH, Bi ZG, Liu DC, Zhang LQ, Yan ZH, Wei YM, Zheng YL (2011) Allelic variation and distribution of HMW glutenin subunit 1Ay in Triticum species. Genet Resour Crop Evol 59:491–497

    Article  Google Scholar 

  • Kilian B, Mammen K, Millet E, Sharma R, Graner A, Salamini F, Hammer K, Özkan H (2011) Aegilops. Wild crop relatives: genomic and breeding resources, pp 1–76

  • Kuraparthy V, Chhuneja P, Dhaliwal HS, Kaur S, Bowden RL, Gill BS (2007) Characterization and mapping of cryptic alien introgression from Aegilops geniculata with new leaf rust and stripe rust resistance genes Lr57 and Yr40 in wheat. Theor Appl Genet 114:1379–1389

    Article  CAS  PubMed  Google Scholar 

  • Lu CM, Lu BR (2005) Molecular characterization of the HMW glutenin genes Dtx1.5+Dty10 from Aegilops tauschii and their PCR-mediated recombinants. Mol Breed 15:247–255

    Article  CAS  Google Scholar 

  • Lutz J, Hsam SLK, Limpert E, Zeller FJ (1995) Chromosomal location of powdery mildew resistance genes in Triticum aestivum L. (common wheat). 2. Genes Pm2 and Pm19 from Aegilops squarrosa L. Heredity 74:152–156

    Article  Google Scholar 

  • Mamluk OF, Slageren MWV (1997) Aegilops spp. as sources of resistance to wheat diseases. In: Birouk A, Rejdali M (eds) Ressources Phytogénétiques et Développement Durable, Actes, Rabat, Maroc, pp 197–202

  • Marais GF, McCallum B, Snyman JE, Pretorius ZA, Marais AS (2005) Leaf rust and stripe rust resistance genes Lr54 and Yr37 transferred to wheat from Aegilops kotschyi. Plant Breed 124:538–541

    Article  CAS  Google Scholar 

  • McIntosh RA, Yamazaki Y, Devos KM, Dubcoveky J, JRogers W, Appels R (2003) Catalogue of gene symbols for wheat (MacGene 2003) (CD-ROM). In: Pogna NE (ed) Proceedings of the 10th international wheat genetics symposium, 4 SIMI, Rome, Italy

  • McIntosh RA, Dubcovsky J, Rogers WJ, Morris C, Appels R, Xia XC (2011) Catalogue of gene symbols for wheat: 2011 supplement. Available online at: http://www.shigen.nig.ac.jp/wheat/komugi/genes/macgene/supplement2011.pdf

  • Miller TE, Reader SM, Ainsworth, Summers RW (1988) The introduction of a major gene for resistance to powdery mildew of wheat, Erysiphe graminis f. sp. tritici, from Aegilops speltoides into wheat, Triticum aestivum. In: Jorna M, Shootmaker L (eds) Cereal breeding related to integrated cereal production: proceedings of the EUCARPIA conference. Pudoc, Wageningen, The Netherlands, pp 179–183

  • Miranda LM, Murphy JP, Marshall D, Cowger C, Leath S (2007) Chromosomal location of Pm35, a novel Aegilops tauschii derived powdery mildew resistance gene introgressed into common wheat (Triticum aestivum L.). Theor Appl Genet 114:1451–1456

    Article  CAS  PubMed  Google Scholar 

  • Mohler V, Bauer C, Schweizer G, Kempf H, Hartl L (2013) Pm50: a new powdery mildew resistance gene in common wheat derived from cultivated emmer. J Appl Genet 54:259–263

    Article  CAS  PubMed  Google Scholar 

  • Payne PI, Lawrence GJ (1983) Catalogue of alleles for the complex gene loci, Glu-A1, Glu-B1and Glu-D1 which code for high molecular weight subunits of glutenin in hexaploid wheat. Cereal Res Com 11:29–35

    Google Scholar 

  • Payne PI, Corfield KG, Blackman JA (1979) Identification of a high-molecular-weight subunit of glutenin whose presence correlates with bread-making quality in wheats of related pedigree. Theor Appl Genet 55:153–159

    Article  CAS  PubMed  Google Scholar 

  • Payne PI, Law CN, Mudd EE (1980) Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theor Appl Genet 58:113–120

    Article  CAS  PubMed  Google Scholar 

  • Payne PI, Corfield KG, Blackman JA (1981) Correlation between the inheritance of certain high-molecular-weight subunits of glutenin and bread-making quality in progenies of six crosses of bread wheat. J Sci Food Agric 32:51–60

    Article  CAS  Google Scholar 

  • Payne PI, Nightingale MA, Krattiger AF, Holt LM (1987) The relationship between HMW glutenin subunit composition and the bread-making quality of British grown wheat varieties. J Sci Food Agri 40(1):51–65

    Article  CAS  Google Scholar 

  • Pena RJ, Zacro-Hernandez J, Mujeeb-Kazi A (1995) Glutenin subunits composition and bread-making quality characteristics of synthetic hexaploid wheats derived from Triticum turgidum. Triticum tauschii (Coss.) Schmal. crosses. J Cereal Sci 21:15–23

    Article  CAS  Google Scholar 

  • Riley RV, Chapman V, Johnson R (1968) The incorporation of alien disease resistance in wheat by genetic interferences with regulation of meiotic chromosome synapsis. Genet Res 12:199–219

    Article  Google Scholar 

  • Saari EE, Prescott JM (1975) A scale for appraising the foliar intensity of wheat diseases. Plant Dis Rep 59:377–380

  • Sears ER (1956) The transfer of leaf-rust resistance from Aegilops umbellulata to wheat. Genetics in plant breeding, Brookhaven Symp. Biology 9:1–22

    Google Scholar 

  • Shewry PR, Halford NG, Tatham AS (1992) High molecular weight subunits of wheat glutenin. J Cereal Sci 15:105–120

    Article  CAS  Google Scholar 

  • Shewry PR, Gilbert SMA, Savage WJ, Tatham AS, Wan YF, Belton PS, Wellner N, D’ovidio R, Békés F, Halford NG (2003) Sequence and properties of HMW subunit 1Bx20 from pasta wheat (Triticum durum) which is associated with poor end use properties. Theor Appl Genet 106:744–750

    CAS  PubMed  Google Scholar 

  • Siddiqui KA, Yosufzai MN (1988) Natural and indused variation for endomorphic traits in the tribe Triticeae. In: Proceedings 7th international wheat genetics symposium, Cambridge, pp 139–144

  • Van Slageren MW (1994) Wild wheats: a monograph of Aegilops L. and Amblyopyrum (Jaub & Spach) Eig (Poaceae). Agricultural University, Wageningen-ICARDA, Aleppo, Syria, p 512

  • Zaharieva M, Monneveux P, Henry M, Rivoal R, Valkoun J, Nachit MM (2001) Evaluation of a collection of wild wheat relative Aegilops geniculata Roth and identification of potential sources for useful traits. Euphytica 119:33–38

    Article  Google Scholar 

  • Zeller FJ, Heun M (1985) The incorporation and characterization of powdery mildew resistance from Aegilops longissima in common wheat (T. aestivum L.). Theor Appl Genet 71:513–517

    Article  CAS  PubMed  Google Scholar 

  • Zeller FJ, Kong LR, Hartl L, Mohler V, Hsam SLK (2002) Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em. Thell.) 7. Gene Pm29 in line Pova. Euphytica 123:187–194

    Article  CAS  Google Scholar 

  • Zhang XY, Wang RRC, Dong YS (1996) RAPD polymorphisms in Aegilops geniculata Roth (Ae. ovata auct. non L.). Genet Resour Crop Evol 43:429–433

    Google Scholar 

  • Zhang XY, Pang BS, You GX, Wang LF, Jia JZ, Dong YC (2002) Allelic variation and genetic diversity at Glu-1 loci in Chinese wheat (Triticum aestivum L.) germplasms. Agric Sci China 1:1074–1082

    Google Scholar 

  • Zhou XL, Han DJ, Chen XM, Gou HL, Guo SJ, Rong L, Wang QL, Huang LL, Kang ZS (2014) Characterization and molecular mapping of stripe rust resistance gene Yr61 in winter wheat cultivar Pindong 34. Theor Appl Genet 127:2349–2358

    Article  CAS  PubMed  Google Scholar 

Download references

Acknowledgments

This research was supported by the National Natural Science Foundation of China (Grant No. 31471481) and the National Key Technology R&D Program (No. 2013BAD01B02-6).

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Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Yajuan Wang, Changyou Wang, Hong Zhang, Hao Li, Xinlun Liu & Wanquan Ji

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  1. Yajuan Wang
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  2. Changyou Wang
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  3. Hong Zhang
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Correspondence to Wanquan Ji.

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Wang, Y., Wang, C., Zhang, H. et al. Identification and evaluation of disease resistance and HMW-GS composition of Aegilops geniculata Roth. Genet Resour Crop Evol 62, 1085–1093 (2015). https://doi.org/10.1007/s10722-015-0217-7

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