Summary
The electrophoretic mobilities of the high-molecular-weight (HMW) subunits of glutenin from 7 varieties were compared by polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate (SDS). In total, 12 subunits were clearly resolved and they had nominal molecular weights of between 95,000 and 140,000. The chromosomes which control their synthesis were determined using monosomic lines and inter-varietal substitution lines. All subunits were shown to be controlled by the homoeologous group 1 chromosomes. Each variety contains between 3 and 5 HMW subunits; two are under the control of the 1D chromosome, 1 or 2 are controlled by chromosome 1B and 0 or 1 by chromosome 1A. The segregation of two 1D-controlled subunits of similar electrophoretic mobilities were analysed in the F2 progeny of crosses between ‘Chinese Spring’ and ‘Holdfast’. The results suggest that the genes which code for the two proteins are allelic.
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Literature
Bietz, J.A.; Huebner, F.R.; Wall, J.S. (1973): Glutenin, the strength protein of wheat flour. Bakers Digest 47, 26–31
Bietz, J.A.; Shepherd, K.W.; Wall, J.S. (1975): Single-kernel analysis of glutenin: use in wheat genetics and breeding. Cereal Chem. 52, 513–532
Blakesley, R.W.; Boezi, J.A. (1977): A new staining technique for proteins in polyacrylamide gels in g Coomassie Brilliant Bleu G250. Analyt. Biochem. 82, 580–582
Brown, J.S.W.; Kemble, R.J.; Law, C.N.; Flavell, R.B. (1980): Control of endosperm proteins in Triticum aestivum L (var. ‘Chinese Spring’) and Aegilops umbellulata Zhuk. by homoeologous group 1 chromosomes. Genetics 93, 189–200
Huebner, F.R.; Wall, J.S. (1976): Fractionation and quantitative differences of glutenin from wheat varieties varying in baking quality. Cereal Chem. 53, 258–269
Hanson, W.D. (1959): Minimum family sizes for the planning of genetic experiments. Agron. J. 51, 711–716
Khan, K.; Bushuk, W. (1977): Studies of glutenin. IX. Subunit composition by sodium dodecyl sulphate — polyacrylamide gel electrophoresis at pH 7.3 and 8.9. Cereal Chem. 54, 588–596
Laemmli, U.K. (1970): Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680–685
Law, C.N.; Worland, A.J. (1973): Aneuploidy in wheat and its uses in genetic analysis. In: Plant Breeding Institute Annual Report, 1972, pp. 25–65. Plant Breeding Institute: Cambridge
Orth, R.A.; Bushuk, W. (1973a): Studies of glutenin. 2. Relation of variety, location of growth, and baking quality to molecular weight distribution of subunits. Cereal Chem. 50, 191–197
Orth, R.A.; Bushuk, W. (1973b): Studies of glutenin. 3. Identification of subunits coded by the D-genome and their relation to breadmaking quality. Cereal Chem. 50, 680–687
Orth, R.A.; Bushuk, W. (1974): Studies of glutenin. 4. Chromosomal location of genes coding for subunits of glutenin of common wheat. Cereal Chem. 51, 118–126
Payne, P.I.; Corfield, K.G. (1979): Subunit composition of wheat glutenin proteins, isolated by gel filtration in a dissociating medium. Planta 145, 83–88
Payne, P.I.; Corfield, K.G.: Blackman, J.A.: (1979): Identification of a high-molecular-weight subunit of glutenin whose presence correlates with bread-making quality in wheats of related pedigree. Theor. Appl. Genet. 55, 153–159
Sears, E.R. (1954): The aneuploids of common wheat. University of Missouri Research Bulletin 572, 3–58
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Payne, P.I., Law, C.N. & Mudd, E.E. Control by homoeologous group 1 chromosomes of the high-molecular-weight subunits of glutenin, a major protein of wheat endosperm. Theoret. Appl. Genetics 58, 113–120 (1980). https://doi.org/10.1007/BF00263101
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DOI: https://doi.org/10.1007/BF00263101