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Identification and fine mapping of molecular markers closely linked to fruit spines size ss gene in cucumber (Cucumis sativus L.)

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Abstract

Fruit spine size is one of the importantly external quality traits effected the economic value of cucumber fruit. Morphological–cytological observation of the fruit spine size phenotype indicated that large spine formation arises from an increasing of spiny pedestal cell number caused by cell division, and best periods to accurately score fruit spine size trait was 4th day before flowering to 7th day after flowering according the continuous observation. Genetic analysis showed that a single dominant gene determined the fruit spine size trait in cucumber. BC1 population (189 individuals) of two inbred lines (large spine PI197088 and small spine SA0422) was used for primary mapping of the SS/ss locus with 7 markers covering an interval of 37.1 cM. An F2 segregating population of 1032 individuals constructed from the same two parents (PI197088 and SA0422) was used to fine mapping of the SS/ss locus. Six new markers linked to the gene were successfully screened for construction of a fine linkage map, in which the SS/ss locus was located in the region flanked by marker SE1 (3 recombinants) and SSR43 (2 recombinants) with a 189 kb physical distance. Markers from this study will be valuable for candidate gene cloning and marker-assisted selection for cucumber breeding.

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Acknowledgements

This study was funded by Shanghai Natural Science Foundation (No. 15ZR1429800), the National Natural Science Foundation of China (No. 31672148), and the Youth Fund of the Natural Science Foundation of Jiangsu Province of China (BK20150232).

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  1. Zhang Weiwei and Chen Yue have contributed equally to this work.

Authors and Affiliations

  1. Department of Plant Science and Technology, Shanghai Vocational College of Agriculture and Forestry, Zhongshan’er Road, Songjiang District, Shanghai, 201699, Shanghai, China

    Zhang Weiwei, Zhou Peng, Bao Wenmin, Xu Taibai, She Weiwei, Xu Liqin & Yu Pinggao

  2. School of Agriculture and Biology, Shanghai Jiaotong University, Dongchuan Road, Minhang District, Shanghai, 200240, Shanghai, China

    Zhang Weiwei, Chen Yue & Pan Junsong

  3. Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province & School of Life Science, Jiangsu Normal University, Xuzhou, 221116, Jiangsu, China

    Yang Xuqin

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  1. Zhang Weiwei
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  2. Chen Yue
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  3. Zhou Peng
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  4. Bao Wenmin
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Correspondence to Yu Pinggao or Pan Junsong.

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Zhang, W., Chen, Y., Zhou, P. et al. Identification and fine mapping of molecular markers closely linked to fruit spines size ss gene in cucumber (Cucumis sativus L.). Euphytica 214, 213 (2018). https://doi.org/10.1007/s10681-018-2251-y

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