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Genetic mapping and QTL analysis of horticultural traits in cucumber (Cucumis sativus L.) using recombinant inbred lines

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Abstract.

A set of 171 recombinant inbred lines (RIL) were developed from a narrow cross in cucumber (Cucumis sativus L.; 2n = 2x = 14) using the determinate (de), gynoecious (F), standard-sized leaf line G421 and the indeterminate, monoecious, little-leaf (ll) line H-19. A 131-point genetic map was constructed using these RILs and 216 F2 individuals to include 14 SSRs, 24 SCARs, 27 AFLPs, 62 RAPDs, 1 SNP, and three economically important morphological [F (gynoecy), de (determinate habit), ll (little leaf)] markers. Seven linkage groups spanned 706 cM with a mean marker interval of 5.6 cM. The location of F and de was defined by genetic linkage and quantitative trait locus (QTL) analysis to be associated with SSR loci CSWCT28 and CSWCTT14 at 5.0 cM and 0.8 cM, respectively. RIL-based QTL analysis of the number of lateral branches in three environments revealed four location-independent factors that cumulatively explained 42% of the observed phenotypic variation. QTLs conditioning lateral branching (mlb1.1), fruit length/diameter ratio (ldr1.2) and sex expression (sex1.2) were associated with de. Sex expression was influenced by three genomic regions corresponding to F and de both on linkage Group 1, and a third locus (sex6.1) on linkage Group 6. QTLs conditioning the number of fruit per plant (fpl1.2), the number of lateral branches (mlb1.4) and fruit length/diameter ratio (ldr1.3) were associated with ll. The potential value of these marker-trait associations (i.e., yield components) for plant improvement is portended by the relatively high LOD scores (2.6 to 13.0) and associated R2 values (1.5% to 32.4%) that are affiliated with comparatively few genetic factors (perhaps 3 to 10).

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Acknowledgements.

This research was partially supported by grant No. IS-2708-96 from the U.S.-Israel Binational Agricultural Research and Development (BARD) Fund.

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Authors and Affiliations

  1. USDA ARS/Dept. of Horticulture, University of Wisconsin-Madison, Madison, WI 53706, USA

    G. Fazio & J. E. Staub

  2. Department of Plant and Animal Sciences, 275 WIDB, Brigham Young University, Provo, UT 84602, USA

    M. R. Stevens

  3. Former graduate student; currently at USDA ARS, Department of Horticultural Sciences, Cornell University, Geneva, NY 14456, USA

    G. Fazio

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  1. G. Fazio
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  2. J. E. Staub
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Correspondence to J. E. Staub.

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Fazio, G., Staub, J.E. & Stevens, M.R. Genetic mapping and QTL analysis of horticultural traits in cucumber (Cucumis sativus L.) using recombinant inbred lines. Theor Appl Genet 107, 864–874 (2003). https://doi.org/10.1007/s00122-003-1277-1

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