Abstract
Mottled/uniform color at the flower end of immature fruit is a highly important external quality trait that affects the market value of cucumber. Genetic analysis of different F2 and backcross populations revealed that one single recessive gene, u (uniform immature fruit color), determines the uniform immature fruit color trait in cucumber. Based on earlier studies, the u locus is located on chromosome 5 (Chr. 5). By combining bulked segregant analysis using 60 published molecular markers on Chr. 5, we found that eight markers are polymorphic and are linked to the u locus. In addition, we developed five new relevant polymorphic simple sequence repeat (SSR) markers between markers SSR16203 and SSR15818. Subsequently, the F2 population (477 individuals) from the cross of S06 (uniform fruit color line) × S94 (mottled fruit color line) was used for fine mapping of the u gene. The u gene was mapped to a 313.2-kb region between markers SSR10 and SSR27, at a genetic distance of 0.8 and 0.5 cM, respectively. Moreover, validity analysis of the codominant markers SSR10 and SSR27 was performed using 50 lines with mottled/uniform fruit color, demonstrating that these two SSR markers can be used for marker-assisted selection of the mottled/uniform fruit color trait in cucumber breeding. The results of this study will facilitate the cloning of the u gene.
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Acknowledgments
We thank Lihuang Zhu (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences CAS) for his technical assistance. This work was supported by grants from the National Key Basic Research Projects (973 Program) (No. 2012CB113900), the National High Technology Research and Development Program of China (863 Program) (No. 2012AA100101) and the Shanghai Graduate Education and Innovation Program (Horticulture).
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Yang, X., Li, Y., Zhang, W. et al. Fine mapping of the uniform immature fruit color gene u in cucumber (Cucumis sativus L.). Euphytica 196, 341–348 (2014). https://doi.org/10.1007/s10681-013-1037-5
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DOI: https://doi.org/10.1007/s10681-013-1037-5