Abstract
Dull/glossy fruit skin is a highly valuable external quality trait that affects the market value of cucumbers. In this study, genetic analysis showed that one single dominant gene, D (dull fruit skin), determines the dull fruit skin trait in cucumber. By combining bulked segregant analysis with 11 published polymorphic molecular markers on chromosome 5, the D/d gene was preliminarily mapped between markers SCZ69 and SSR16203, at genetic distances of 0.3 and 0.6 cM, respectively. Subsequently, a larger F2 (S06 × S94) population (842 individuals in total) was used for high-resolution mapping of the D/d gene. Finally, the D/d gene was fine-mapped between markers SSR37 and SSR112, at a physical distance of 244.9 kb (containing 31 candidate genes), using eight newly developed polymorphic simple sequence repeat (SSR) markers between SCZ69 and SSR16203. Based on semi-quantitative RT-PCR analysis, the possible candidate gene D was identified as Csa016880 or Csa016887. Meanwhile, validity analysis of the markers SSR37 and SSR112 was performed with 72 dull/glossy fruit lines, and showed that the two co-dominant SSR markers could be used for marker-assisted selection of the dull/glossy fruit trait in cucumber breeding. Moreover, this study will be helpful for cloning of the D gene in cucumber.
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Acknowledgments
We thank Lihuang Zhu (Institute of Genetics and Developmental Biology, Chinese Academy of Sciences CAS) for his technical assistance. We also thank Dr. Sanwen Huang (Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences CAAS) for kindly providing the cucumber genome data. This work was supported by grants from the National High Technology Research and Development Program of China (863 Program) (No. 2012AA100101), the National Key Basic Research Projects (973 Program) (No. 2012CB113900) and the National Natural Science Foundation of China (No. 31271291).
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Yang, X., Zhang, W., Li, Y. et al. High-resolution mapping of the dull fruit skin gene D in cucumber (Cucumis sativus L.). Mol Breeding 33, 15–22 (2014). https://doi.org/10.1007/s11032-013-9927-8
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DOI: https://doi.org/10.1007/s11032-013-9927-8