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Breeding for grapevine downy mildew resistance: a review of “omics” approaches

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Abstract

Downy mildew (DM) is one of the most destructive diseases affecting viticulture, especially in temperate-humid climates. This pathogen is able to differentially attack leaves and grapes, and is currently controlled with repeated applications of fungicides that lead to environmental pollution, development of resistant strains, residual toxicity, and pathogen pressure. The use of resistant grapevine varieties, obtained through breeding programs, represents a potential alternative to control grapevine DM, although this approach is constrained by the limits of natural resistance. In this review, we describe the latest breakthroughs achieved in the exploitation of the host–pathogen interaction, which have led to the identification of DM resistance loci, with associated molecular markers, that can be employed to efficiently screen hybrid grapevines and improve (pre-)breeding programs. The development of controlled infection protocols and contemporary “omics” approaches (next generation sequencing/genomics, QTLomics, transcriptomics, proteomics and metabolomics) integrated with comparative studies are shedding light onto the early host responses to DM attack and the complex plant defence mechanisms that are triggered.

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Acknowledgements

The authors apologize to the scientists that are not cited because of space limitation. They wish to thank Marco Stefanini (FEM) for helpful discussions and Elena Zini (Laimburg Institute) for useful suggestions on Table 2. Daniele Buonassisi acknowledges funding from a FEM PhD fellowship at University of Udine, Monica Colombo from the Cariplo foundation (project GrAptaResistance, Integrated research on industrial biotechnologies 2015), and Michele Perazzolli from the European Unions Seventh Framework Programme for research, technological development and demonstration under Grant agreement No. 324416 (project INNOVA, theme FP7-PEOPLE-2012-IAPP).

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Authors and Affiliations

  1. Research and Innovation Centre, Fondazione Edmund Mach, via E. Mach 1, 38010, San Michele all’Adige, TN, Italy

    Daniele Buonassisi, Monica Colombo, Chiara Dolzani, Elisa Peressotti, Riccardo Velasco, Michele Perazzolli & Silvia Vezzulli

  2. Department of Agricultural, Food, Environmental and Animal Sciences, University of Udine, via delle Scienze 206, 33100, Udine, Italy

    Daniele Buonassisi

  3. Consiglio per la Ricerca in Agricoltura e l’Analisi dell’Economia Agraria - Centro di Ricerca per la Viticoltura, viale XXVIII Aprile 26, 31015, Conegliano, TV, Italy

    Daniele Migliaro

  4. Dipartimento di Bioscienze, Università degli Studi di Milano, via Celoria 26, 20133, Milano, Italy

    Chiara Mizzotti & Simona Masiero

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  1. Daniele Buonassisi
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  2. Monica Colombo
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  10. Silvia Vezzulli
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Correspondence to Silvia Vezzulli.

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This article is part of the Topical Collection on Plant Breeding: the Art of Bringing Science to Life. Highlights of the 20th EUCARPIA General Congress, Zurich, Switzerland, 29 August–1 September 2016

Edited by Roland Kölliker, Richard G. F. Visser, Achim Walter & Beat Boller

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Buonassisi, D., Colombo, M., Migliaro, D. et al. Breeding for grapevine downy mildew resistance: a review of “omics” approaches. Euphytica 213, 103 (2017). https://doi.org/10.1007/s10681-017-1882-8

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