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Identification and mapping of PmSE5785, a new recessive powdery mildew resistance locus, in synthetic hexaploid wheat

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Abstract

Powdery mildew of wheat (Triticum aestivum L.) caused by Blumeria graminis f. sp. tritici, is a major fungal disease in many areas of the world. Synthetic hexaploids, created from durum wheat and Aegilops tauschii are important sources of beneficial genes for wheat improvement. Synthetic wheat ‘SE5785’ was found resistant to powdery mildew at the seedling and adult stages. Genetic analyses of the F2 population and F2:3 families developed from a cross of ‘SE5785’ with the susceptible common wheat, ‘Xiaoyan 22’, revealed that resistance in ‘SE5785’ was controlled by a single recessive gene. Bulked segregant analysis was used to map the powdery mildew resistance locus. The resulting genetic map comprised of markers Xcfd62, Xbarc59, PmSE5785, Xgwm539, and Xwmc817 spanned 16.3 cM. Markers Xbarc59 and Xgwm539 were tightly linked to the resistance locus at genetic distances of 3.6 and 4.0 cM, respectively. ‘Chinese Spring’ and its nulli-tetrasomic lines confirmed that PmSE5785 was located on chromosome 2D. Because no gene for powdery mildew resistance had ever been located on this chromosome in the genus Triticum, this must be a new locus, therefore, designated PmSE5785. N07228-1 and N07228-2 with large seeds and powdery mildew resistance were selected from the progeny of the cross ‘SE5785’/‘Xiaoyan 22’. These new lines can serve as sources of resistance to powdery mildew in wheat breeding.

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References

  • Cowger C, Miranda L, Griffey C, Hall M, Murphy JP, Maxwell J (2012) Wheat powdery mildew. In: Sharma I (ed) Disease resistance in wheat. CAB International, Oxfordshire, pp 84–119

    Chapter  Google Scholar 

  • Cox TS, Raupp WJ, Wilson BS, Gill B, Leath S, Bockus WW, Browder LE (1992) Resistance to foliar diseases in a collection of Triticum tauschii germplasm. Plant Dis 76:1061–1064

    Article  Google Scholar 

  • Gill BS, Raupp WJ (1987) Direct gene transfers from Aegilops squarrosa L. to hexaploid wheat. Crop Sci 27:445–450

    Article  Google Scholar 

  • Gill BS, Friebe BR, White FF (2011) Alien introgressions represent a rich source of genes for crop improvement. Proc Natl Acad Sci USA 108:7657–7658

    Article  PubMed  PubMed Central  Google Scholar 

  • Hao YF, Parks R, Cowger C, Wang YY, Bland D, Murphy JP, Guedira M, Brown-Guedira G, Johnson J (2015) Molecular characterization of a new powdery mildew resistance gene Pm54 in soft red winter wheat. Theor Appl Genet 128:465–476

    Article  PubMed  CAS  Google Scholar 

  • He RL, Chang ZJ, Yang ZJ, Yuan ZY, Zhan HX, Zhang XJ, Liu JX (2009) Inheritance and mapping of powdery mildew resistance gene Pm43 introgressed from Thinopyrum intermedium into wheat. Theor Appl Genet 118:1173–1180

    Article  PubMed  CAS  Google Scholar 

  • Hsam SLK, Huang XQ, Zeller FJ (2001) Chromosomal location of genes for resistance to powdery mildew in common wheat (Triticum aestivum L. em Thell.). 6. Alleles at the Pm5 locus. Theor Appl Genet 102:127–133

    Article  CAS  Google Scholar 

  • Hua W, Liu ZJ, Zhu J, Xie CJ, Yang T, Zhou YL, Duan XY, Sun QX (2009) Identification and genetic mapping of pm42, a new recessive wheat powdery mildew resistance gene derived from wild emmer (Triticum turgidum var. dicoccoides). Theor Appl Genet 119:223–230

    Article  PubMed  CAS  Google Scholar 

  • Huang XQ, Wang LX, Xu MX, Röder MS (2003) Microsatellite mapping of the powdery mildew resistance gene Pm5e in common wheat (Triticum aestivum L.). Theor Appl Genet 106:858–865

    PubMed  CAS  Google Scholar 

  • Kerber ER (1987) Resistance to leaf rust in hexaploid wheat: Lr32, a third gene derived from Triticum tauschii. Crop Sci 27:204–206

    Article  Google Scholar 

  • Lapochkina IF, Solomatin DA, Serezhkina GV, Grishina EE, Vishnyakova KS, Pukhalskii VA (1996) Common wheat lines with genetic material from Aegilops speltoides Tausch. Genetika 32:1651–1656

    Google Scholar 

  • Lebsock KL, Briggle LW (1974) Gene Pm5 for resistance to Erysiphe graminis f. sp. tritici in Hope wheat. Crop Sci 14:561–563

    Article  Google Scholar 

  • Liu RH, Meng JL (2003) MapDraw: a Microsoft Excel macro for drawing genetic linkage maps based on given genetic linkage data. Hereditas (Beijing) 25:317–321

    Google Scholar 

  • Liu ZY, Sun QX, Ni ZF, Nevo E, Yang TM (2002) Molecular characterization of a novel powdery mildew resistance gene Pm30 in wheat originating from wild emmer. Euphytica 123:21–29

    Article  CAS  Google Scholar 

  • Lutz J, Hsam SLK, Limpert E, Zeller FJ (1994) Powdery mildew resistance in Aegilops tauschii Coss. and synthetic hexaploid wheats. Genet Res Crop Evol 41:151–158

    Article  Google Scholar 

  • Lutz J, Hsam SLK, Limpert E, Zeller FJ (1995) Chromosomal location of powdery mildew resistance genes in Triticum aestivum L. (common wheat). 2. Genes Pm2 and Pm19 from Aegilops squarrosa L. Heredity 74:152–156

    Article  Google Scholar 

  • Ma H, Singh P, Mujeeb-Kazi A (1995) Resistance to stripe rust in Triticum turgidum, T. tauschii and their synthetic hexaploids. Euphytica 82:117–124

    Article  Google Scholar 

  • McDonald BA, Linde C (2002) The population genetics of plant pathogens and breeding strategies for durable resistance. Euphytica 124:163–180

  • McIntosh RA, Baker EP (1970) Cytogenetic studies in wheat IV Chromosomal location and linkage studies involving the Pm2 locus for powdery mildew resistance. Euphytica 19:71–77

    Article  Google Scholar 

  • Michelmore RW, Paran I, Kesseli RV (1991) Identification of markers linked to disease-resistance genes by bulked segregant analysis-a rapid method to detect markers in genomic regions by using segregating populations. Proc Natl Acad Sci USA 88:9828–9832

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Miller TE, Reader SM, Ainsworth CC, Summers RW (1988) The introduction of a major gene for resistance to powdery mildew of wheat, Erysiphe graminis f. sp. tritici, from Aegilops speltoides into wheat, Triticum aestivum. In: Joma ML, Slootmaker LAJ et al (eds) Cereal breeding related to integrated cereal production. Pudoc, Wageningen, pp 179–183

    Google Scholar 

  • Miranda LM, Murphy JP, Leath S, Marshall DS (2006) Pm34: a new powdery mildew resistance gene transferred from Aegilops tauschii Coss. to common wheat (Triticum aestivum L.). Theor Appl Genet 114:1497–1504

    Article  CAS  Google Scholar 

  • Miranda LM, Murphy JP, Marshall D, Cowger C, Leath S (2007) Chromosomal location of Pm35, a novel Aegilops tauschii derived powdery mildew resistance gene introgressed into common wheat (Triticum aestivum L.). Theor Appl Genet 114:1451–1456

    Article  PubMed  CAS  Google Scholar 

  • Mujeeb-Kazi A, Rosas V, Roldan S (1996) Conservation of the genetic variation of Triticum tauschii (Coss.) Schmalh. (Aegilops squarrosa auct. non L.) in synthetic hexaploid wheats (T. turgidum L. s.lat. × T. tauschii; 2n = 6 × = 42, AABBDD) and its potential utilization for wheat improvement. Genet Resour Crop Evol 43:129–134

    Article  Google Scholar 

  • Nguyen AT, Iehisa JCM, Kajimura T, Murai K, Takumi S (2013) Identification of quantitative trait loci for flowering-related traits in the D genome of synthetic hexaploid wheat lines. Euphytica 192:401–441

    Article  CAS  Google Scholar 

  • Parks R, Carbone I, Murphy JP, Marshall D, Cowger C (2008) Virulence structure of the Eastern US wheat powdery mildew population. Plant Dis 92:1074–1082

    Article  Google Scholar 

  • Petersen S, Lyerly JH, Worthington ML, Parks WR, Cowger C, Marshall DS, Brown-Guedira G, Murphy JP (2015) Mapping of powdery mildew resistance gene Pm53 introgressed from Aegilops speltoides into soft red winter wheat. Theor Appl Genet 128:303–312

    Article  PubMed  CAS  Google Scholar 

  • Rong JK, Millet E, Manisterski J, Feldman M (2000) A new powdery mildew resistance gene: introgression from wild emmer into common wheat and RFLP-based mapping. Euphytica 115:121–126

    Article  CAS  Google Scholar 

  • Schneider DM, Heun M, Fischbeck G (1991) Inheritance of the powdery mildew resistance gene Pm9 in relation to Pm1 and Pm2 of wheat. Plant Breed 107:161–164

    Article  Google Scholar 

  • Schneider A, Molnar I, Molnar-Lang M (2008) Utilisation of Aegilops (goatgrass) species to widen the genetic diversity of cultivated wheat. Euphytica 163:1–19

    Article  CAS  Google Scholar 

  • Sheng B (1988) Grades of resistance to powdery mildew classified by different phenotypes of response in the seeding stage of wheat. Plant Prot 1:49

    Google Scholar 

  • Singrün Ch, Hsam SLK, Zeller FJ, Wenzel G, Mohler V (2004) Localization of a novel recessive powdery mildew resistance gene from common wheat line RD30 in the terminal region of chromosome 7AL. Theor Appl Genet 109:210–214

    Article  PubMed  CAS  Google Scholar 

  • Van Ooijen JW (2006) JionMap 4.0, Software for the calculation of genetic linkage maps in experimental populations. Kyazma BV, Wageningen

  • Wang YJ, Wang CY, Liu XL, Zhang H, Ji WQ (2010) Identification and evaluation of disease resistance and HMW-GS of synthetic wheat. Acta Agric Boreali Sin 25(6):122–127

    Google Scholar 

  • Wang YJ, Wang CY, Zhang H, Yue ZN, Liu XL, Ji WQ (2013) Genetic analysis of wheat (Triticum aestivum L.) and related species with SSR markers. Genet Resour Crop Evol 60:1105–1117

    Article  CAS  Google Scholar 

  • Xiao MG, Song FJ, Jiao JF, Wang XM, Xu HX, Li HJ (2013) Identification of the gene Pm47 on chromosome 7BS conferring resistance to powdery mildew in the Chinese wheat landrace Hongyanglazi. Theor Appl Genet 126:1397–1403

    Article  PubMed  CAS  Google Scholar 

  • Xu H, Yao G, Li Xiong, Yang L, Jiang Y, Fu B, Zhao W, Zhang Z, Zhang C, Ma Z (2008) Identification and mapping of pm2026: a recessive powdery mildew resistance gene in an einkorn (Triticum monococcum L.) accession. Theor Appl Genet 117:471–477

    Article  PubMed  CAS  Google Scholar 

  • Xue F, Ji WQ, Wang CY, Zhang H, Yang BJ (2012) High-density mapping and marker development for the powdery mildew resistance gene PmAS846 derived from wild emmer wheat (Triticum turgidum var. dicoccoides). Theor Appl Genet 124:1549

    Article  PubMed  CAS  Google Scholar 

  • Zaharieva M, Ayana NG, Al Hakimi A, Misra SC, Monneveux P (2010) Cultivated emmer wheat (Triticum dicoccon Schrank), an old crop with promising future: a review. Genet Resour Crop Evol 57:937–962

    Article  Google Scholar 

Download references

Acknowledgments

This research was supported by the innovation project of science and technology of Shaanxi province of China (2015KTZDNY01-01-02), the Key Technologies R&D Program of China (2013BAD01B02-6) and the Zhongying Tang Breeding Fund of Northwest A & F University (2014-2015).

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Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Yangling, 712100, Shaanxi, China

    Yajuan Wang, Changyou Wang, Wei Quan, Xiujuan Jia, Ying Fu, Hong Zhang, Xinlun Liu, Chunhuan Chen & Wanquan Ji

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  1. Yajuan Wang
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  2. Changyou Wang
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  3. Wei Quan
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  4. Xiujuan Jia
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Correspondence to Wanquan Ji.

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Wang, Y., Wang, C., Quan, W. et al. Identification and mapping of PmSE5785, a new recessive powdery mildew resistance locus, in synthetic hexaploid wheat. Euphytica 207, 619–626 (2016). https://doi.org/10.1007/s10681-015-1560-7

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  • DOI: https://doi.org/10.1007/s10681-015-1560-7

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