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Modulation of lipase B from Candida antarctica properties via covalent immobilization on eco-friendly support for enzymatic kinetic resolution of rac-indanyl acetate

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Abstract

In this study, the modulation of enzymatic biocatalysts were developed by the use of lipase B from Candida antarctica covalently immobilized on an eco-friendly support, cashew apple bagasse, activated with 10% glycidol–ethylenediamine–glutaraldehyde (GEG) under different immobilization strategies (5 mM or 100 mM ionic strength and in absence or presence of 0.5% (v/v) Triton X-100). The biocatalysts were characterized for thermal and organic solvents stabilities and compared with the soluble enzyme. The biocatalysts were then applied to the hydrolysis of the rac-indanyl acetate (2:1 ratio enzyme/substrate) at pH 7.0 and 30 °C for 24 h. For all the strategies evaluated, GEG promoted kinetic resolution of rac-indanyl acetate with maximum conversion (50%) and led to (R)-indanol with excellent enantiomeric excess (97%), maintaining the maximum conversion for five consecutive cycles of hydrolysis. Therefore, the use of cashew apple bagasse has proved to be a promising eco-friendly support for enzyme immobilization, since it resulted in stable biocatalysts for enzymatic kinetic resolution.

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Acknowledgements

We gratefully acknowledge the financial support of Brazilian Agencies for Scientific and Technological Development, Fundação Cearense de Apoio ao Desenvolvimento Científico e Tecnológico (FUNCAP), project number BP3-0139-00005.01.00/18, Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), project number 422942/2016-2 and 311062/2019-9, Coordenação de Aperfeiçoamento de Ensino Superior (CAPES)—Finance Code 001. M. C. de Mattos thanks CNPq for research sponsorships (Process: 306043/2018-1).

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  1. Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Fortaleza, CE, CEP 60455760, Brazil

    Ticiane C. de Souza, Jouciane de Sousa Silva, Paula J. M. Lima, Carlos A. C. G. Neto, Rodolpho R. C. Monteiro, Maria Valderez P. Rocha, Marcos C. de Mattos, José C. S. dos Santos & Luciana R. B. Gonçalves

  2. Departamento de Química Orgânica e Inorgânica, Universidade Federal do Ceará, Campus do Pici, Fortaleza, CE, CEP 60455970, Brazil

    Thiago de Sousa Fonseca

  3. Instituto de Engenharias e Desenvolvimento Sustentável, Universidade da Integração Internacional da Lusofonia Afro-Brasileira, Redenção, CE, CEP 62785000, Brazil

    José C. S. dos Santos

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  1. Ticiane C. de Souza
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Correspondence to José C. S. dos Santos or Luciana R. B. Gonçalves.

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de Souza, T.C., de Sousa Fonseca, T., de Sousa Silva, J. et al. Modulation of lipase B from Candida antarctica properties via covalent immobilization on eco-friendly support for enzymatic kinetic resolution of rac-indanyl acetate. Bioprocess Biosyst Eng 43, 2253–2268 (2020). https://doi.org/10.1007/s00449-020-02411-8

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