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Pros and Cons in Various Immobilization Techniques and Carriers for Enzymes

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Abstract

In recent years, enzyme immobilization technology has been developed, and studies on immobilized enzyme materials have become very prominent. With the immobilization technique, enzymes and compatible carrier materials are combined or enzyme crystals/aggregates are used in a carrier-free fashion, by physical, chemical, or biochemical methods. As a kind of biocatalyst, immobilized enzymes can catalyze certain chemical reactions with high selectivity and high efficiency under relatively mild reaction conditions and eliminate pollution to the environment. Considering the current status and applications of immobilized enzyme technology and materials emerging in the last 5 years, this mini-review introduces the advantages and disadvantages of various enzyme immobilization techniques with carriers as well as the pros and cons of different materials for immobilization. The future prospects of immobilization technology and carrier materials are outlined, aiming to provide a reference for further research and applications of sustainable technology.

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Copyright 2021 American Chemical Society

Fig. 4

Reproduced with permission from ref. [95]. Copyright by Spring Nature Publishing Group

Fig. 5

Reproduced with permission from ref. [100]. Creative Commons CC BY license

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Funding

This work was supported by the National Natural Science Foundation of China (Grant number 21973061) and Zhejiang Provincial Science and Technology Innovation Program for College Students (Grant number 2023R465014).

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Authors and Affiliations

  1. College of Chemistry and Chemical Engineering, Shaoxing University, Shaoxing, 312000, Zhejiang Province, China

    Yong Jiang, Jinxia Zheng, Mengna Wang, Wanqi Xu, Yiquan Wang, Li Wen & Jian Dong

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  1. Yong Jiang
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  2. Jinxia Zheng
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  3. Mengna Wang
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  4. Wanqi Xu
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  5. Yiquan Wang
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  6. Li Wen
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  7. Jian Dong
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Contributions

All authors contributed to the study conception and design. The first draft of the manuscript was written by YJ, JZ, MW, WX, and YW. YJ, JZ, and LW performed the literature search and revised and reviewed the manuscript. JD performed the literature search, data analysis, revised and reviewed the manuscript, and supervised the study. All authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Jian Dong.

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Jiang, Y., Zheng, J., Wang, M. et al. Pros and Cons in Various Immobilization Techniques and Carriers for Enzymes. Appl Biochem Biotechnol (2024). https://doi.org/10.1007/s12010-023-04838-7

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