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Enhanced biocatalytic activity of immobilized Pseudomonas cepacia lipase under sonicated condition

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Abstract

The present work reports the use of biocatalyst and ultrasound for greener synthesis of cinnamyl propionate. The lipase Pseudomonas cepacia was immobilized on a copolymer of hydroxypropyl methyl cellulose and polyvinyl alcohol. This biocatalyst was u sed for ultrasound-assisted synthesis of cinnamyl propionate with the detailed optimization of various reaction parameters. Besides this, protocol was extended to synthesize various industrially important propionate esters. In addition to this, different enzyme-kinetic parameters such as r max and K m(vinyl propionate), K m(cinnamyl alcohol) and K i(cinnamyl alcohol) were studied which presented ordered bi–bi mechanism with an inhibition by cinnamyl alcohol. The developed biocatalyst demonstrated enhancement in catalytic activity and recyclability up to five recycles. Moreover, the biocatalyst was tested to investigate the effects of sonication via various characterization techniques such as scanning electron microscopy, thermogravimetry, and water content analysis.

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Acknowledgments

Author Kirtikumar is grateful to the Department of Biotechnology-DBT, India, File No.: (BT/PR4192/PID/6/623/2011), and the Council of Scientific and Industrial Research-CSIR, India File No.: (09/991(0015)/2011-EMR-I), for providing research fund and fellowship, respectively.

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Authors and Affiliations

  1. Department of Chemistry, Institute of Chemical Technology, Matunga, Mumbai, 400 019, India

    Kirtikumar C. Badgujar, Poorna A. Pai & Bhalchandra M. Bhanage

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  1. Kirtikumar C. Badgujar
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  2. Poorna A. Pai
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  3. Bhalchandra M. Bhanage
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Correspondence to Bhalchandra M. Bhanage.

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Badgujar, K.C., Pai, P.A. & Bhanage, B.M. Enhanced biocatalytic activity of immobilized Pseudomonas cepacia lipase under sonicated condition. Bioprocess Biosyst Eng 39, 211–221 (2016). https://doi.org/10.1007/s00449-015-1505-5

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  • DOI: https://doi.org/10.1007/s00449-015-1505-5

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