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Molecular genetic analysis of five spike-related traits in wheat using RIL and immortalized F2 populations

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Abstract

Kernel number per spike is one of the most important yield components of wheat. To map QTLs related to kernel number including spike length (SPL), spikelet number per spike (SPN), fertile spikelet number (FSPN), sterile spikelet number (SSPN) and compactness, and to characterize the inheritance modes of the QTLs and two-locus interactions, 136 recombinant inbred lines (RILs) derived from ‘Nanda2419’ x ‘Wangshuibai’ and an immortalized F2 population (IF2) generated by randomly permutated intermating of these RILs were investigated. QTL mapping made use of the previously constructed over 3300 cM linkage map of the RIL population. Three, five, two, two and six chromosome regions were identified, respectively, for their association with SPL, SPN, FSPN, SSPN, and compactness in at least two of the three environments examined. All compactness QTLs but one shared the respective intervals of QSpn.nau-5A and the SPL QTLs. Xcfd46–Xwmc702 interval on chromosome 7D was related to all traits but SSPN and had consistently the largest effects. The fact that not all the compactness QTL intervals were related to both SPL and SPN indicates that compactness is regulated by different mechanisms. Interval coincidence between QTLs of SPL and SPN and between QTLs of FSPN and SSPN was minimal. For all the traits, favorable alleles exist in both parents. Inheritance modes from additiveness to overdominance of the QTLs were revealed and two-locus interactions were detected, implying that the traits studied are under complex genetic control. The results could contribute to wheat yield improvement and better use of Wangshuibai and Nanda2419 the two special germplasms in wheat breeding program.

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Acknowledgments

This project was partially supported by ‘973’ program (2006CB101702), NFSC program (30430440, 30025030), and ‘863’ program (2003AA207100).

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Authors and Affiliations

  1. The Applied Plant Genomics Lab and National Key Lab of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China

    Zhengqiang Ma, Dongmei Zhao, Caiqin Zhang, Zhengzhi Zhang, Shulin Xue, Feng Lin, Zhongxin Kong, Dagang Tian & Qingyun Luo

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  1. Zhengqiang Ma
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  2. Dongmei Zhao
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  4. Zhengzhi Zhang
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  7. Zhongxin Kong
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  9. Qingyun Luo
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Correspondence to Zhengqiang Ma.

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Communicated by R. Hagemann.

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Ma, Z., Zhao, D., Zhang, C. et al. Molecular genetic analysis of five spike-related traits in wheat using RIL and immortalized F2 populations. Mol Genet Genomics 277, 31–42 (2007). https://doi.org/10.1007/s00438-006-0166-0

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  • DOI: https://doi.org/10.1007/s00438-006-0166-0

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