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Precise mapping Fhb5, a major QTL conditioning resistance to Fusarium infection in bread wheat (Triticum aestivum L.)

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Abstract

Qfhi.nau-5A is a major quantitative trait locus (QTL) against Fusarium graminearum infection in the resistant wheat germplasm Wangshuibai. Genetic analysis using BC3F2 and BC4F2 populations, derived from selfing two near-isogenic lines (NIL) heterozygous at Qfhi.nau-5A that were developed, respectively, with Mianyang 99-323 and PH691 as the recurrent parent, showed that Qfhi.nau-5A inherited like a single dominant gene. This QTL was thus designated as Fhb5. To fine map it, these two backcross populations and a recombinant inbred line (RIL) population derived from Nanda2419 × Wangshuibai were screened for recombinants occurring between its two flanking markers Xbarc56 and Xbarc100. Nineteen NIL recombinants were identified from the two backcross populations and nine from the RIL population. In the RIL recombinant selection process, selection against Fhb4 present in the RIL population was incorporated. Genotyping these recombinant lines with ten markers mapping to the Xbarc56–Xbarc100 interval revealed four types of Mianyang 99-323-derived NIL recombinants, three types of PH691-derived NIL recombinants, and four types of RIL recombinants. In different field trials, the percentage of infected spikes of these lines displayed a distinct two-peak distribution. The more resistant class had over 55% less infection than the susceptible class. Common to these resistant genotypes, the 0.3-cM interval flanked by Xgwm304 and Xgwm415 or one of these two loci was derived from Wangshuibai, while none of the susceptible recombinants had Wangshuibai chromatin in this interval. This interval harboring Fhb5 was mapped to the pericentromeric C–5AS3-0.75 bin through deletion bin mapping. The precise localization of Fhb5 will facilitate its utilization in marker-assisted wheat breeding programs.

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Acknowledgments

This study was partially supported by “973” program (2010CB125902), the National Natural Science Foundation of China programs (31000535, 31030054, 30721140555), funds for transgenic plants (2008ZX08002-001 and 2009ZX08009-049B), the Natural Science Foundation of Jiangsu Province of China program (BK2009305), the Research Fund for the Doctoral Program of Higher Education of China program (20100097120042), the “111” project B08025, and the PAPD project of Jiangsu Higher Education Institutions.

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  1. Crop Genomics and Bioinformatics Centre and National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing, 210095, Jiangsu, China

    Shulin Xue, Feng Xu, Mingzhi Tang, Yan Zhou, Guoqiang Li, Xia An, Feng Lin, Haibin Xu, Haiyan Jia, Lixia Zhang, Zhongxin Kong & Zhengqiang Ma

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  1. Shulin Xue
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  2. Feng Xu
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Correspondence to Zhengqiang Ma.

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Communicated by F. Ordon.

S. L. Xue and F. Xu contributed equally to this article.

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Xue, S., Xu, F., Tang, M. et al. Precise mapping Fhb5, a major QTL conditioning resistance to Fusarium infection in bread wheat (Triticum aestivum L.). Theor Appl Genet 123, 1055–1063 (2011). https://doi.org/10.1007/s00122-011-1647-z

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