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Identification and characterization of a novel powdery mildew resistance gene PmG3M derived from wild emmer wheat, Triticum dicoccoides

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Abstract

Powdery mildew, caused by Blumeria graminis f. sp. tritici (Bgt) is one of the most important wheat diseases worldwide. Wild emmer wheat, Triticum turgidum ssp. dicoccoides, the tetraploid ancestor (AABB) of domesticated bread and durum wheat, harbors many important alleles for resistance to various diseases, including powdery mildew. In the current study, two tetraploid wheat mapping populations, derived from a cross between durum wheat (cv. Langdon) and wild emmer wheat (accession G-305-3M), were used to identify and map a novel powdery mildew resistance gene. Wild emmer accession G-305-3M was resistant to all 47 Bgt isolates tested, from Israel and Switzerland. Segregation ratios of F2 progenies and F6 recombinant inbred line (RIL) mapping populations, in their reactions to inoculation with Bgt, revealed a Mendelian pattern (3:1 and 1:1, respectively), indicating the role of a single dominant gene derived from T. dicoccoides accession G-305-3M. This gene, temporarily designated PmG3M, was mapped on chromosome 6BL and physically assigned to chromosome deletion bin 6BL-0.70-1.00. The F2 mapping population was used to construct a genetic map of the PmG3M gene region consisted of six simple sequence repeats (SSR), 11 resistance gene analog (RGA), and two target region amplification polymorphism (TRAP) markers. A second map, constructed based on the F6 RIL population, using a set of skeleton SSR markers, confirmed the order of loci and distances obtained for the F2 population. The discovery and mapping of this novel powdery mildew resistance gene emphasize the importance of the wild emmer wheat gene pool as a source for crop improvement.

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Acknowledgments

This study was supported by The Israel Science Foundation grants #608/03 and #205/08. We also acknowledge The Israel Science Foundation equipment grants #048/99, #1478/04 and #1719/08. The authors wish to thank J. Dubcovsky for providing the 6BS RSLs, and A. Fahoum and O. Shalish for their skillful assistance in the genotyping.

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Authors and Affiliations

  1. Department of Evolutionary and Environmental Biology, Institute of Evolution, Faculty of Natural Sciences, University of Haifa, Mt. Carmel, 31905, Haifa, Israel

    Weilong Xie, Roi Ben-David, Bin Zeng & Tzion Fahima

  2. Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK), 06466, Gatersleben, Germany

    Marion S. Röder

  3. Department of Plant Pathology and Microbiology, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, 76100, Rehovot, Israel

    Amos Dinoor

  4. Molecular Biology and Ecology of Plants, The Institute for Cereal Crops Improvement, Tel-Aviv University, 69978, Tel-Aviv, Israel

    Assaf Distelfeld

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  1. Weilong Xie
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  2. Roi Ben-David
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  3. Bin Zeng
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  4. Assaf Distelfeld
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Correspondence to Tzion Fahima.

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Communicated by B. Friebe.

First authorship shared by W. Xie and R. Ben-David.

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Xie, W., Ben-David, R., Zeng, B. et al. Identification and characterization of a novel powdery mildew resistance gene PmG3M derived from wild emmer wheat, Triticum dicoccoides . Theor Appl Genet 124, 911–922 (2012). https://doi.org/10.1007/s00122-011-1756-8

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