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Recent biotechnological advances and future prospective of Bacillus licheniformis as microbial cell factories

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Abstract

Bacillus licheniformis is a characteristic Gram-positive bacterium originally found in soil. This microorganism has long been utilised as a workhorse for production of industrial enzymes or high value-added chemicals. With ever-increasing understanding on this strain and the maturation of the genetic technique, important advances have recently been made in developing B. licheniformis as an excellent chassis cell for synthetic biology. Here, we provide an overview of updated understanding on genome information, anaerobic metabolism, industrial applications of this strain. The state-of-art B. licheniformis genetics, especially its synthetic biology advances in biosensor, expression system and artificial metabolic pathways are illustrated. Finally, perspectives are offered for the limitations and challenges to be addressed to improve B. licheniformis as microbial cell factories.

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Acknowledgements

This work was supported by National Key Research & Development Program of China (2018YFA0900504, 2020YFA0907700, and 2018YFA0900300), the National Natural Foundation of China (31401674), the National First-Class Discipline Program of Light Industry Technology and Engineering (LITE2018-22), and the Top-notch Academic Programs Project of Jiangsu Higher Education Institutions.

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  1. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214000, Jiangsu, People’s Republic of China

    Hehe He, Yupeng Zhang, Guiyang Shi & Youran Li

  2. National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214000, Jiangsu, People’s Republic of China

    Hehe He, Yupeng Zhang, Guiyang Shi & Youran Li

  3. Jiangsu Provisional Research Center for Bioactive Product Processing Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, Jiangsu Province, People’s Republic of China

    Hehe He, Yupeng Zhang, Guiyang Shi & Youran Li

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  1. Hehe He
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He, H., Zhang, Y., Shi, G. et al. Recent biotechnological advances and future prospective of Bacillus licheniformis as microbial cell factories. Syst Microbiol and Biomanuf 3, 521–532 (2023). https://doi.org/10.1007/s43393-023-00162-7

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