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Regulation of acetoin and 2,3-butanediol utilization in Bacillus licheniformis

  • Applied Microbial and Cell Physiology
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Abstract

The acoABCL and acuABC operons of Bacillus licheniformis DSM13 are strongly induced at the transcriptional level during glucose starvation conditions. Primer extension analyses of this study indicate that the acoABCL operon is controlled by a sigmaL-dependent promoter and the acuABC operon by a sigmaA-dependent promoter. Transcription at the acoA promoter is repressed by glucose but induced by acetoin as soon as the preferred carbon source glucose is exhausted. The acuA promoter shows a similar induction pattern, but its activity is independent from the presence of acetoin. It is demonstrated that the acoABCL operon is mainly responsible for acetoin and 2,3-butanediol degradation in B. licheniformis.

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Acknowledgements

This work was supported by a scholarship from the Ministry of Education and Training of Vietnam (MOET) and the Institute of Marine Biotechnology (IMaB) to Trung Nguyen Thanh. Results of this study were generated in the frame of the Competence Network “Genome Research on Bacteria” financed by the German Federal Ministry of Education and Research (BMBF) (BiotechGenomik 0313751B and GenomikDesign). We thank Friedhelm Meinhardt for providing the MW3 mutant strain. We also thank Hoi Le Thi for her support in vector constructions.

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Authors and Affiliations

  1. Pharmazeutische Biotechnologie, Institut für Pharmazie, Ernst-Moritz-Arndt-Universität, Friedrich-Ludwig-Jahn-Straße 17, 17487, Greifswald, Germany

    Trung Nguyen Thanh, Britta Jürgen & Thomas Schweder

  2. Lehrstuhl für Mikrobiologie, TU-München, 85354, Freising, Germany

    Melanie Bauch & Armin Ehrenreich

  3. Pharmazeutische Biologie, Institut für Pharmazie, Ernst-Moritz-Arndt-Universität, 17487, Greifswald, Germany

    Manuel Liebeke & Michael Lalk

  4. Henkel AG & Co. KGaA, WRC Biotechnology, 40191, Düsseldorf, Germany

    Stefan Evers & Karl-Heinz Maurer

  5. Institut für Mikrobiologie, Ernst-Moritz-Arndt-Universität, 17487, Greifswald, Germany

    Haike Antelmann & Michael Hecker

  6. ZIK FunGene, Ernst-Moritz-Arndt-Universität, 17487, Greifswald, Germany

    Britta Jürgen, Manuel Liebeke, Michael Lalk, Florian Ernst, Georg Homuth, Michael Hecker & Thomas Schweder

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  1. Trung Nguyen Thanh
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  2. Britta Jürgen
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Correspondence to Thomas Schweder.

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Thanh, T.N., Jürgen, B., Bauch, M. et al. Regulation of acetoin and 2,3-butanediol utilization in Bacillus licheniformis . Appl Microbiol Biotechnol 87, 2227–2235 (2010). https://doi.org/10.1007/s00253-010-2681-5

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  • DOI: https://doi.org/10.1007/s00253-010-2681-5

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