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Two α-Arabinofuranosidases from Chrysoporthe cubensis and Their Effects on Sugarcane Bagasse Saccharification

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Abstract

Two α-arabinofuranosidases from the fungus Chrysoporthe cubensis COAD 3356 were partially purified, identified, characterized, and applied to the sugarcane bagasse saccharification to evaluate the potential of these enzymes to increase the sugar production from lignocellulosic biomass. The α-arabinofuranosidases were classified on GH51 (α-Ara1) and GH54/CBM42 (α-Ara2) families. After sugarcane bagasse saccharification, using the commercial cellulase-rich cocktail supplemented with α-Ara2 (15 U/g), there was an increase of 1.6, 3.9, and 6.1 times in the release of glucose, xylose, and arabinose, respectively. On the other hand, there was no increase in sugar release with α-Ara1 supplementation under the same saccharification conditions. The enzymes presented maximum activity at pH 4.0, and 60 °C. Both α-Ara1 and α-Ara2 were thermostable at 50 °C, presenting half-life values of 68 and 77 h, respectively. The enzyme α-Ara2 presented higher KMapp for synthetic substrate ρNP-α-arabinofuranoside (1.38 mmol/L) and wheat arabinoxylan (1.28 mmol/L) when compared with α-Ara1. A new fungal α-arabinofuranosidase structure, still little described in the GH51 family, was predicted. Furthermore, the results indicated that α-Ara2 is a promising molecule to be used to supplement cocktails for lignocellulose degradation.

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Data Availability

The datasets generated during and/or analyzed during the current study are available from the corresponding author upon reasonable request.

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Acknowledgements

We acknowledge the Brazilian institutions Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for the scholarship granted to the first author, Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the resources provided to complete this experiment. We would also like to thank Felipe Bini for reviewing the English-language version of the manuscript.

Funding

This work was supported by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors and Affiliations

  1. Nucleus of Research in Biological Sciences, Federal University of Ouro Preto, Ouro Preto, MG, 35400-000, Brazil

    Mariana Furtado Granato de Albuquerque

  2. Department of Natural Sciences, Federal University of São João Del-Rei, São João Del-Rei, MG, 36301-160, Brazil

    Maíra Nicolau de Almeida

  3. Department of Biochemistry and Molecular Biology, Federal University of Viçosa, Vicosa, MG, 36570-000, Brazil

    Murillo Peterlini Tavares, Rafaela Inês de Souza Ladeira Ázar, Lílian da Silva Fialho, Sebastião Tavares de Rezende & Valéria Monteze Guimarães

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  1. Mariana Furtado Granato de Albuquerque
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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by Mariana Furtado Granato de Albuquerque, Murillo Peterlini Tavares, Lílian da Silva Fialho, and Rafaela Inês de Souza Ladeira Ázar. The first draft of the manuscript was written by Mariana Furtado Granato de Albuquerque, and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Valéria Monteze Guimarães.

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de Albuquerque, M.F.G., de Almeida, M.N., Tavares, M.P. et al. Two α-Arabinofuranosidases from Chrysoporthe cubensis and Their Effects on Sugarcane Bagasse Saccharification. Bioenerg. Res. (2024). https://doi.org/10.1007/s12155-024-10721-y

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