Abstract
The presence of xylan is a detriment to the enzymatic saccharification of cellulose in lignocelluloses. The inhibition of the processive cellobiohydrolase Cel7A by soluble wheat arabinoxylan is shown here to increase by 50 % following enzymatic treatment with a commercially-purified α-l-arabinofuranosidase. The enhanced inhibitory effect was shown by T2 relaxation time measurements via low field NMR to coincide with an increasing degree of constraint put on the water in xylan solutions. Furthermore, quartz crystal micro-balance with dissipation experiments showed that α-l-arabinofuranosidase treatment considerably increased the rate and rigidity of arabinoxylan mass association with cellulose. These data also suggest significant xylan–xylan adlayer formation occurs following initial binding of debranched arabinoxylan. From this, we speculate the inhibitory effects of xylan to cellulases may result from reduced enzymatic access via the dense association of xylan with cellulose.
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References
Almond A (2005) Towards understanding the interaction between oligosaccharides and water molecules. Carbohydr Res 340:907–920
Baumann MJ, Borch K, Westh P (2011) Xylan oligosaccharides and cellobiohydrolase I (TrCel7A) interaction and effect on activity. Biotechnol Biofuels 4:45
Chandler D (2005) Interfaces and the driving force of hydrophobic assembly. Nature 437:640–647
Cremer D, Pople JA (1975) A general definition of ring puckering coordinates. J Am Chem Soc 97:1354–1358
Dashnau JL, Sharp KA, Vanderkooi JM (2005) Carbohydrate intramolecular hydrogen bonding cooperativity and its effect on water structure. J Phys Chem B 109:24152–24159
Dillon SR, Dougherty RC (2002) Raman studies of the solution structure of univalent electrolytes in water. J Phys Chem A 106:7647–7650
Engelund ET, Thygesen LG, Svensson S, Hill CAS (2013) A critical discussion of the physics of wood–water interactions. Wood Sci Technol 47:141–161
Felby C, Thygesen LG, Kristensen JB, Jorgensen H, Elder T (2008) Cellulose-water interactions during enzymatic hydrolysis as studied by time domain NMR. Cellulose 15(5):703–710
Himmel ME, Ding SY, Johnson DK, Adney WS, Nimlos MR, Brady JW, Foust TD (2007) Biomass recalcitrance: engineering plants and enzymes for biofuels production. Science 315(5813):804–807
Jeoh T, Ishizawa CI, Davis MF, Himmel ME, Adney WS, Johnson DK (2007) Cellulase digestibility of pretreated biomass is limited by cellulose accessibility. Biotechnol Bioeng 98(1):112–122
Kabel MA, Bos G, Zeevalking J, Voragen AGJ, Schols HA (2007a) Effect of pretreatment severity on xylan solubility and enzymatic breakdown of the remaining cellulose from wheat straw. Biores Technol 98(10):2034–2042
Kabel M, Van den Borne H, Vincken JP, Voragen AGJ, Schols HA (2007b) Structural differences of xylans effect their interaction with cellulose. Carbohydr Polym 69:94–105
Kim S, Holtzapple M (2006) Effect of structural features on enzyme digestibility of corn stover. Bioresour Technol 97:583–591
Köhnke T, Ostlund A, Brelid H (2011) Adsorption of arabinoxylan on cellulosic surfaces: influence of degree of substitution and substitution pattern on adsorption characteristics. Biomacromolecules 12:2633–2641
Matthews JF, Skopec CE, Mason PE, Zuccato P, Torget RW, Sugiyama J, Himmel ME, Brady JW (2006) Computer simulation studies of microcrystalline cellulose Ib. Carbohydr Res 341:138–152
Mrevlishvili GM (1998) Low-temperature heat capacity of biomacromolecules and the entropic cost of bound water in proteins and nucleic acids (DNA). Thermochim Acta 308:49–54
Pitkanen L, Viikari L, Tenkanen M, Tuomainen P (2009) Comprehensive multidetector HPSEC study on solution properties of cereal arabinoxylans in aqueous and DMSO solutions. Biomacromolecules 10:1962–1969
Qing Q, Yang B, Wyman CE (2010) Xylooligomers are strong inhibitors of cellulose hydrolysis by enzymes. Biores Technol 101(24):9624–9630
Roberts KM, Lavenson DM, Tozzi EJ, McCarthy MJ, Jeoh T (2011) The effects of water interactions in cellulose suspensions on mass transfer and saccharification efficiency at high solids loadings. Cellulose 18:759–773
Scheller HV, Ulvskov P (2010) Hemicelluloses. Annu Rev Plant Biol 61:263–289
Selig MJ, Vinzant TB, Himmel ME, Decker SR (2009) The effect of lignin removal by alkaline peroxide pretreatment on the susceptibility of corn stover to purified cellulolytic and hemicellulolytic enzymes. Appl Biochem Biotechnol 155(1–3):397–406
Selig MJ, Hsieh CC, Thygesen L, Himmel ME, Felby C, Decker SR (2012) Considering water availability and the effect of solute concentration on high solids saccharification of lignocellulosic biomass. Biotechnol Prog 28(6):1478–1490
Selig MJ, Thygesen LG, Felby C (2014) Constrained water and the inhibition of cellulose saccharification by lignocellulosic plant polymers. Biotech Biofuels in press: editorially accepted October 2014
Sternemalm E, Höije A, Gatenholm P (2008) Effect of arabinose substitution on the material properties of arabinoxylan films. Carbohydr Res 343:753–757
Vogel J (2008) Unique aspects of the grass cell wall. Curr Opin Plant Biol 11:301–307
Wolfe J, Bryant G, Koster KL (2002) What is ‘unfreezable water’, how unfreezable is it and how much is there? CryoLetters 23:157–166
Yang B, Wyman CE (2004) Effect of xylan and lignin removal by batch and flowthrough pretreatment on the enzymatic digestibility of corn stover cellulose. Biotechnol Bioeng 86(1):88–95
Zhang J, Tang M, Viikari L (2012) Xylans inhibit enzymatic hydrolysis of lignocellulosic materials by cellulases. Biores Technol 121:8–12
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Selig, M.J., Thygesen, L.G., Felby, C. et al. Debranching of soluble wheat arabinoxylan dramatically enhances recalcitrant binding to cellulose. Biotechnol Lett 37, 633–641 (2015). https://doi.org/10.1007/s10529-014-1705-0
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DOI: https://doi.org/10.1007/s10529-014-1705-0