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Purification and Characterization of Xylanases from the Fungus Chrysoporthe cubensis for Production of Xylooligosaccharides and Fermentable Sugars

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Abstract

Xylanases from the pathogen fungus Chrysoporthe cubensis were produced under solid state fermentation (SSF) using wheat bran as carbon source. The enzymatic extracts were submitted to ion exchange (Q Sepharose) and gel filtration chromatography methods (Sephadex S-200) for purification. The xylanases were divided into three groups: P1 showed better performance at 60 °C and pH 4.0, P2 at 55 °C and pH 3.0, and P3 at 80 °C and pH 3.0. Oat spelt xylan was the best substrate hydrolyzed by P1 and P3, while beechwood xylan was better degraded by P2. Carboxymethyl cellulose (CMC) and p-nitrophenyl-β-d-xylopyranoside (p-NPβXyl) were not hydrolyzed by any of the xylanases. The K M ’ or K M values, using oat spelt xylan as substrate, were 2.65 mg/mL for P1, 1.81 mg/mL for P2, and 1.18 mg/mL for P3. Xylobiose and xylotriose were the main xylooligosaccharides of oat spelt xylan degradation, indicating that the xylanases act as endo-β-1,4-xylanases. Xylanases also proved to be efficient for hydrolysis of sugarcane bagasse when used as supplement of a commercial cocktail due to the increase of the reducing sugar release.

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Acknowledgments

We thank the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG) and the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for the financial support and the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for providing scholarships.

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Authors and Affiliations

  1. Departamento de Bioquímica e Biologia Molecular, BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, 36.570-000, Brazil

    Kamila de Sousa Gomes, Gabriela P. Maitan-Alfenas, Lorena G. A. de Andrade, Daniel Luciano Falkoski, Valéria Monteze Guimarães & Sebastião Tavares de Rezende

  2. Departamento de Fitopatologia, BIOAGRO, Universidade Federal de Viçosa, Viçosa, MG, 36.570-000, Brazil

    Acelino C. Alfenas

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  1. Kamila de Sousa Gomes
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Correspondence to Gabriela P. Maitan-Alfenas.

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de Sousa Gomes, K., Maitan-Alfenas, G.P., de Andrade, L.G.A. et al. Purification and Characterization of Xylanases from the Fungus Chrysoporthe cubensis for Production of Xylooligosaccharides and Fermentable Sugars. Appl Biochem Biotechnol 182, 818–830 (2017). https://doi.org/10.1007/s12010-016-2364-5

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