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Exogenous 24-epibrassinolide regulates antioxidant and pesticide detoxification systems in grapevine after chlorothalonil treatment

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Abstract

The influence of 24-epibrassinolide (EBR) on the regulation of antioxidant and pesticide detoxification systems in grape seedlings (Vitis vinifera L.) was investigated. The grapevine leaves were pretreated with different concentrations of EBR (0.10, 0.21 and 0.42 μM) before spraying with chlorothalonil (CHT) (600 times diluent). Compared with the control, CHT treatment showed an adverse effect on osmoregulation substances (soluble protein and free proline) and increased the content of malondialdehyde (MDA), activities of antioxidant enzymes and detoxification enzymes as well as the content of some non-enzymatic antioxidants. Compared to the CHT treatment alone, application of three concentrations of EBR resulted in an overall enhancement of these enzyme activities, soluble protein and free proline contents and reduction in MDA content. In addition, the expression of some key genes involved in CHT degradation including glutathione synthetase (GSH), glutathione S-transferase (GST), glutathione reductase (GR), P450 monooxygenase (P450) and MRP-type transporter (MRP) was analyzed. The transcriptional level of these genes increased in response to CHT treatment with the exception of GSH. EBR treatment of 0.10 μM could effectively promote expression of most genes and accelerate CHT degradation by 29 % as compared to only CHT treatment in grapevine leaves.

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Abbreviations

APX:

Ascorbate peroxidase

AsA:

Ascorbic acid

CAT:

Catalase

CHT:

Chlorothalonil

EBR:

24-Epibrassinolide

EDTA:

Ethylene diamine tetraacetic acid

MDA:

Malondialdehyde

GR:

Glutathione reductase

GSH:

Reduced glutathione

GST:

Glutathione transferase

POD:

Peroxidase

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

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Acknowledgments

This study was supported by the National Technology System for Grape Industry (CARS-30-zp-9), the Natural Science Foundation of Shaanxi Province (2011JM3004). The authors gratefully acknowledge these institutions for funding.

Author contributions

Conceived and designed the experiments: Zhumei Xi, Wrote the manuscript and performed the experiments: Zhizhen Wang, Yue Jiang, Contributed reagents/materials/analysis tools: Xiaoqin Peng, Shiyan Xu, Hui Zhang, Jiangman Gao.

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    Authors and Affiliations

    1. College of Enology, Northwest A&F University, Yangling, 712100, Shaanxi, People’s Republic of China

      Zhizhen Wang, Yue Jiang, Xiaoqin Peng, Hui Zhang, Jiangman Gao & Zhumei Xi

    2. Shaanxi Provincial Fruit Tree Propagation Center, Yangling, 712100, Shaanxi, People’s Republic of China

      Shiyan Xu

    3. Shaanxi Engineering Research Center for Viti-Viniculture, Yangling, 712100, Shaanxi, People’s Republic of China

      Zhumei Xi

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    1. Zhizhen Wang
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    Correspondence to Zhumei Xi.

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    Zhizhen Wang and Yue Jiang have contributed equally to this work.

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    Wang, Z., Jiang, Y., Peng, X. et al. Exogenous 24-epibrassinolide regulates antioxidant and pesticide detoxification systems in grapevine after chlorothalonil treatment. Plant Growth Regul 81, 455–466 (2017). https://doi.org/10.1007/s10725-016-0223-6

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