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Brassinosteroid-mediated evaluation of antioxidant system and nitrogen metabolism in two contrasting cultivars of Vigna radiata under different levels of nickel

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Abstract

The role of 28-homobrassinolide (HBL) in countering nickel-induced oxidative damage through overexpression of antioxidant enzymes and proline in Vigna radiata has been investigated. Two varieties of V. radiata, one sensitive to Ni (PDM-139) and the other tolerant to Ni (T-44), were sown in the soil fed with different levels (0, 50, 100 or 150 mg kg−1) of Ni, and at 29-day stage, foliage of plants was applied with deionized water (control), 10−8 or 10−6 M of HBL. The plants were sampled at 45-day stage of growth to assess various physiological as well as biochemical characteristics. The remaining plants were allowed to grow up to maturity to study the yield characteristics. The growth traits, leghemoglobin, nitrogen and carbohydrate content in the nodules, leaf chlorophyll content, photosynthesis efficiency, leaf water potential, activities of nitrate reductase, carbonic anhydrase and nitrogenase decreased proportionately with the increasing concentrations of nickel, whereas electrolyte leakage, various antioxidant enzymes viz. catalase, peroxidase and superoxide dismutase and accumulation of proline increased at 45-day stage. However, the exogenously applied HBL to the nickel-stressed or non-stressed plants improved growth, nodulation and photosynthesis and further enhanced the various antioxidant enzymes viz. catalase, peroxidase and superoxide dismutase and accumulation of proline. The deleterious impact of Ni on the plants was concentration dependent where HBL applied to the foliage induced overexpression of antioxidant enzyme and accumulation of proline (osmolyte) which could have conferred tolerance to Ni up to 100 mg kg−1, resulting in improved growth, nodulation, photosynthesis and yield attributes.

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Acknowledgments

M. Yusuf gratefully acknowledges the financial assistance rendered by the Science and Engineering Research Board (SERB), Department of Science and Technology (DST), New Delhi, India, in a form of Young Scientists (SB/FT/LS-210-2012).

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Authors and Affiliations

  1. Department of Agricultural Microbiology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, India

    Iqbal Ahmad

  2. Plant Physiology Section, Department of Botany, Faculty of Life Sciences, Aligarh Muslim University, Aligarh, India

    Mohammad Yusuf, Qazi Fariduddin & Aqil Ahmad

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  1. Mohammad Yusuf
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  2. Qazi Fariduddin
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Correspondence to Qazi Fariduddin.

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Yusuf, M., Fariduddin, Q., Ahmad, I. et al. Brassinosteroid-mediated evaluation of antioxidant system and nitrogen metabolism in two contrasting cultivars of Vigna radiata under different levels of nickel. Physiol Mol Biol Plants 20, 449–460 (2014). https://doi.org/10.1007/s12298-014-0259-x

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