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Brassinolide enhances cold stress tolerance of fruit by regulating plasma membrane proteins and lipids

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Abstract

How to enhance fruit tolerance to cold stress is an important biological interest. In this paper, we found that mango (Mangifera indica L.) fruit treated with 10 μM brassinolide (BL) showed a higher tolerance to cold temperature of 5 °C. Further, we compared the changes in expression profiles of plasma membrane (PM) proteins and the corresponding gene expressions between BL-treated and control fruit. Fourteen differential proteins were positively identified by mass spectrometry, and were categorized into four groups, including transport, cellular biogenesis, defense and stress response, and unknown function. Among them, four proteins (remorin, abscisic stress ripening-like protein, type II SK2 dehydrin, and temperature-induced lipocalin) and genes encoding these proteins were up-regulated in BL treatment under cold stress. Moreover, we found that PM lipids in BL-treated fruit showed lower phase transition temperature and higher unsaturation degree, leading to higher fluidity under low temperature. These findings ascertain that PM proteins and lipids are involved in BL-mediated responses to cold stress in mango fruit, and provide novel evidence that BL plays an important role in regulating cold stress tolerance in fruit.

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Abbreviations

2-DE:

Two-dimensional gel electrophoresis

BL:

Brassinolide

BRs:

Brassinosteroids

CI:

Chilling injury

CBB:

Coomassie brilliant blue

DBI:

Double bond index

DSC:

Differential scanning calorimetry

EPR:

Electron paramagnetic resonance

FAs:

Fatty acids

IEF:

Isoelectric focusing

PM:

Plasma membrane

UFAs:

Unsaturated fatty acids

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (Grant No. 31030051) and the National Basic Research Program of China (973 Program, Grant No. 2011CB100604), and the CAS/SAFEA International Partnership Program for Creative Research Teams (Grant No. 20090491019).

Conflict of interest

The authors have declared that no competing interests exist.

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Authors and Affiliations

  1. Key Laboratory of Plant Resources, Institute of Botany, Chinese Academy of Sciences, 20 Nanxincun, Xiangshan, Haidian District, Beijing, 100093, China

    Boqiang Li, Changfeng Zhang, Baohua Cao, Guozheng Qin, Weihao Wang & Shiping Tian

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  1. Boqiang Li
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  2. Changfeng Zhang
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  3. Baohua Cao
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  4. Guozheng Qin
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  5. Weihao Wang
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  6. Shiping Tian
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Corresponding author

Correspondence to Shiping Tian.

Additional information

B. Li and C. Zhang contributed equally to this work.

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Li, B., Zhang, C., Cao, B. et al. Brassinolide enhances cold stress tolerance of fruit by regulating plasma membrane proteins and lipids. Amino Acids 43, 2469–2480 (2012). https://doi.org/10.1007/s00726-012-1327-6

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  • DOI: https://doi.org/10.1007/s00726-012-1327-6

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