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Efficient production of ε-poly-l-lysine from cassava bagasse hydrolysate used as carbon source by Streptomyces albulus US3-18

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Abstract

The production of ε-poly-l-lysine (ε-PL) from cassava bagasse hydrolysate (CBH) by Streptomyces albulus US3-18 was investigated in this study. With 30 g/L glucose from CBH, 1.30 g/L ε-PL and 10.68 g/L biomass were obtained in shake flask fermentation. Interestingly, the two values were increased by 14.0% and 21.5%, respectively, compared to the control (1.14 g/L and 8.79 g/L). Simultaneously, the activities of four key enzymes of ε-PL synthesis during CBH fermentation were enhanced to varying degrees. In batch fermentation of 5-L bioreactor, 3.39 g/L ε-PL and 10.17 g/L DCW were harvested with 40 g/L glucose from CBH. The combination of fed-batch fermentation with two-stage pH strategy significantly increased ε-PL titer and biomass to 37.41 g/L and 41.0 g/L, respectively. Moreover, eleven volatile components were detected in CBH by GC–MS, and 6-pentyl-α-pyrone (6PP) was first identified as the most abundant volatile ingredient. The results in CBH fermentation demonstrated that S. albulus US3-18 exhibited high tolerance to these volatile byproducts. Using ICP–MS, the calcium concentration in CBH was determined as 195.0 mg/(kg hydrolyzate), and cobalt, copper, lead, chromium, mercury and arsenic were not detected. By adding 0.05 g/L CaCl2 to M3G medium, ε-PL yield was improved by 28.0%, indicating calcium was one of the factors for the enhanced ε-PL production. The study provides a reference for the efficient production of ε-PL from low-cost agricultural residues.

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Funding

This work was supported by the National Natural Science Foundation of China (No. 21676173), the Agricultural Infrastructure Project of Suzhou Science and Technology Development Plan (No. SNG2017053), the Research Fund Project of Suzhou University of Science and Technology (No. XKZ201411), and the Natural Science Foundation of Jiangsu Province (No. BK20210864).

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  1. Jiaolong Fu and Cong Li have contributed equally to this work.

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  1. School of Chemistry and Life Sciences, Suzhou University of Science and Technology, Suzhou, 215009, Jiangsu, People’s Republic of China

    Jiaolong Fu, Cong Li, Xin Ju, Jing Bai, Yunfeng Zhou, Yi Zhang, Yue Wang, Zilong Sun, Cuiying Hu, Liangzhi Li & Lilian Ji

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Jiaolong Fu and Cong Li contributed equally to this work. Jiaolong Fu: Conceptualization, Writing—review & editing, Funding acquisition; Cong Li: Methodology, Investigation, Validation, Formal analysis, Writing—original draft; Xin Ju: Conceptualization, Writing—review & editing; Jing Bai: Writing—review & editing; Yunfeng Zhou: Validation; Yi Zhang: Data curation; Yue Wang: Writing—original draft; Zilong Sun: Data curation; Cuiying Hu: Methodology; Liangzhi Li: Writing—review & editing; Lilian Ji: Supervision.

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Fu, J., Li, C., Ju, X. et al. Efficient production of ε-poly-l-lysine from cassava bagasse hydrolysate used as carbon source by Streptomyces albulus US3-18. Bioprocess Biosyst Eng 45, 1407–1419 (2022). https://doi.org/10.1007/s00449-022-02755-3

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